<abstract xmlns="http://www.w3.org/1999/xhtml">

Synthetic detergents which have a major role in environmental pollution accumulate over time and reach levels that harm nature. The surfactants which are abundantly used as cleaning components are discharged into the Van Lake with the sewage water. These chemicals accumulating in the lake may reach a level that could affect the only fish species of the lake, the Van fish. This study aimed to determine the antioxidant levels of Van fish hepatocyte cell culture medium treated with sodium lauryl sulphate (SLS) and to assess the DNA damage. The effect of SLS was assessed by its dose (1x10−5, 1x10-6, 1x10−7 M) and treatment time (24 h, 48 h). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and DNA damage (8-OHdG) were determined in the SLS hepatocyte culture. SOD and GSH-Px were higher on 24 h and 48 h compared to the control group. A significant increase was observed in CAT level in the first 24 h, especially in 1x10−6 and 1x10-5 M concentration. At 48 h, it was observed that the CAT level decreased significantly as the concentration increased. It was determined that MDA and 8-OHdG levels increased depending on concentration and time. In conclusion, different concentrations of SLS affected antioxidant levels in the primary hepatocyte culture of Van Fish and were found to cause an increase in the levels of MDA and 8-OHdG.
</abstract>

Synthetic detergents which have a major role in environmental pollution accumulate over time and reach levels that harm nature. The surfactants which are abundantly used as cleaning components are discharged into the Van Lake with the sewage water. These chemicals accumulating in the lake may reach a level that could affect the only fish species of the lake, the Van fish. This study aimed to determine the antioxidant levels of Van fish hepatocyte cell culture medium treated with sodium lauryl sulphate (SLS) and to assess the DNA damage. The effect of SLS was assessed by its dose (1x10−5, 1x10-6, 1x10−7 M) and treatment time (24 h, 48 h). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and DNA damage (8-OHdG) were determined in the SLS hepatocyte culture. SOD and GSH-Px were higher on 24 h and 48 h compared to the control group. A significant increase was observed in CAT level in the first 24 h, especially in 1x10−6 and 1x10-5 M concentration. At 48 h, it was observed that the CAT level decreased significantly as the concentration increased. It was determined that MDA and 8-OHdG levels increased depending on concentration and time. In conclusion, different concentrations of SLS affected antioxidant levels in the primary hepatocyte culture of Van Fish and were found to cause an increase in the levels of MDA and 8-OHdG.

Toxic Effects of Sodium Lauryl Sulfate on Antioxidant Defense System and DNA Damage in Fish Primary Hepatocyte Cultures

Department of Chemistry, Faculty of Science

Department of Biology, Faculty of Science

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution 4.0 International License.

Synthetic detergents which have a major role in environmental pollution accumulate over time and reach levels that harm nature. The surfactants which are...

Toxic Effects of Sodium Lauryl Sulfate on Antioxidant Defense System and DNA Damage in Fish Primary Hepatocyte Cultures

Published Online: Nov 23, 2022

Page range: 169 - 175

Received: Sep 22, 2021

Accepted: Sep 05, 2022

DOI: https://doi.org/10.2478/macvetrev-2022-0027

Keywords
SLS, fish, DNA damage, antioxidants, MDA

© 2022 Aslı Ç. Yeltekin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Toxic Effects of Sodium Lauryl Sulfate on Antioxidant Defense System and DNA Damage in Fish Primary Hepatocyte Cultures

Published Online: Nov 23, 2022

Page range: 169 - 175

Received: Sep 22, 2021

Accepted: Sep 05, 2022

DOI: https://doi.org/10.2478/macvetrev-2022-0027

KeywordsSLS, fish, DNA damage, antioxidants, MDA

© 2022 Aslı Ç. Yeltekin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
SLS, fish, DNA damage, antioxidants, MDA