The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon
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Fig. 1.
Diagram of the experimental design for evaluating monocyte-derived macrophage (MDM) activity in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. T – time point; PBMC – peripheral blood mononuclear cells; PPP – platelet-poor plasma; P-PRP – pure platelet-rich plasma with no or a low number of leukocytes; L-PRP –platelet-rich plasma with a high number of leukocytes
Fig. 2.
Comparison of the effect of blood-derived product stimulation on macrophage response in blood drawn from animals at three time points in an ovine model of silicon-doped diamond-like carbon–coated implantation in tibia. (a) superoxide generation; (b) NO generation; (c) arginase-1 activity; (d) urea/NO ratio. NS – non-stimulated macrophages; PPP – platelet-poor plasma–stimulated macrophages; P-PRP – macrophages stimulated with pure platelet-rich plasma with no or a low number of leukocytes; L-PRP – macrophages stimulated with platelet-rich plasma with a high number of leukocytes; T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation; * – significant difference to NS at each time point (P-value < 0.05)
Fig. 3.
Representative microphotographs of morphological changes in macrophages in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. Optical microscopy ×40 carried out 48 h after stimulation with different blood-derived products at three time points. A – naïve macrophages after DMEM and 10% BCF supplementation; B, D – rounded cells visible in all cultures after platelet-poor plasma (PPP) stimulation; C, E–H – pseudopodia (blue arrows) induced after stimulation by platelet-rich plasma with a high number of leukocytes (L-PRP) or with platelet-rich plasma with no or a low number of leukocytes (P-PRP); T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation