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Journal of Veterinary Research
Volumen 68 (2024): Edición 1 (March 2024)
Acceso abierto
The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon
Bartłomiej Szymczak
Bartłomiej Szymczak
,
Andrzej Junkuszew
Andrzej Junkuszew
,
Krzysztof Patkowski
Krzysztof Patkowski
,
Tomasz Szponder
Tomasz Szponder
,
Dominika Nguyen Ngoc
Dominika Nguyen Ngoc
,
Beata Drzewiecka
Beata Drzewiecka
,
Aleksandra Sobczyńska-Rak
Aleksandra Sobczyńska-Rak
y
Joanna Wessely-Szponder
Joanna Wessely-Szponder
| 23 mar 2024
Journal of Veterinary Research
Volumen 68 (2024): Edición 1 (March 2024)
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Publicado en línea:
23 mar 2024
Páginas:
167 - 174
Recibido:
28 jul 2023
Aceptado:
15 ene 2024
DOI:
https://doi.org/10.2478/jvetres-2024-0003
Palabras clave
blood-derived products
,
macrophages
,
Si-DLC implant
,
ovine model
© 2024 Bartłomiej Szymczak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1.
Diagram of the experimental design for evaluating monocyte-derived macrophage (MDM) activity in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. T – time point; PBMC – peripheral blood mononuclear cells; PPP – platelet-poor plasma; P-PRP – pure platelet-rich plasma with no or a low number of leukocytes; L-PRP –platelet-rich plasma with a high number of leukocytes
Fig. 2.
Comparison of the effect of blood-derived product stimulation on macrophage response in blood drawn from animals at three time points in an ovine model of silicon-doped diamond-like carbon–coated implantation in tibia. (a) superoxide generation; (b) NO generation; (c) arginase-1 activity; (d) urea/NO ratio. NS – non-stimulated macrophages; PPP – platelet-poor plasma–stimulated macrophages; P-PRP – macrophages stimulated with pure platelet-rich plasma with no or a low number of leukocytes; L-PRP – macrophages stimulated with platelet-rich plasma with a high number of leukocytes; T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation; * – significant difference to NS at each time point (P-value < 0.05)
Fig. 3.
Representative microphotographs of morphological changes in macrophages in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. Optical microscopy ×40 carried out 48 h after stimulation with different blood-derived products at three time points. A – naïve macrophages after DMEM and 10% BCF supplementation; B, D – rounded cells visible in all cultures after platelet-poor plasma (PPP) stimulation; C, E–H – pseudopodia (blue arrows) induced after stimulation by platelet-rich plasma with a high number of leukocytes (L-PRP) or with platelet-rich plasma with no or a low number of leukocytes (P-PRP); T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation