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The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon

Bartłomiej Szymczak

Bartłomiej Szymczak

Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, University of Life SciencesLublin, Poland
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Szymczak, Bartłomiej
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Andrzej Junkuszew

Andrzej Junkuszew

Department of Animal Breeding and Agricultural Consulting, Faculty of Animal Sciences and Bioeconomy, University of Life SciencesLublin, Poland
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Junkuszew, Andrzej
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Krzysztof Patkowski

Krzysztof Patkowski

Department of Animal Breeding and Agricultural Consulting, Faculty of Animal Sciences and Bioeconomy, University of Life SciencesLublin, Poland
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Patkowski, Krzysztof
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Tomasz Szponder

Tomasz Szponder

Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life SciencesLublin, Poland
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Szponder, Tomasz
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Dominika Nguyen Ngoc

Dominika Nguyen Ngoc

Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, University of Life SciencesLublin, Poland
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Ngoc, Dominika Nguyen
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Beata Drzewiecka

Beata Drzewiecka

Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, University of Life SciencesLublin, Poland
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Drzewiecka, Beata
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Aleksandra Sobczyńska-Rak

Aleksandra Sobczyńska-Rak

Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life SciencesLublin, Poland
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Sobczyńska-Rak, Aleksandra
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Joanna Wessely-Szponder

Joanna Wessely-Szponder

Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, University of Life SciencesLublin, Poland
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Wessely-Szponder, Joanna
  
23. März 2024
The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon's Cover Image
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Online veröffentlicht: 23. März 2024
Seitenbereich: 167 - 174
Eingereicht: 28. Juli 2023
Akzeptiert: 15. Jan. 2024
DOI: https://doi.org/10.2478/jvetres-2024-0003
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© 2024 Bartłomiej Szymczak et al., published by Sciendo
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Fig. 1.

Diagram of the experimental design for evaluating monocyte-derived macrophage (MDM) activity in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. T – time point; PBMC – peripheral blood mononuclear cells; PPP – platelet-poor plasma; P-PRP – pure platelet-rich plasma with no or a low number of leukocytes; L-PRP –platelet-rich plasma with a high number of leukocytes
Diagram of the experimental design for evaluating monocyte-derived macrophage (MDM) activity in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. T – time point; PBMC – peripheral blood mononuclear cells; PPP – platelet-poor plasma; P-PRP – pure platelet-rich plasma with no or a low number of leukocytes; L-PRP –platelet-rich plasma with a high number of leukocytes

Fig. 2.

Comparison of the effect of blood-derived product stimulation on macrophage response in blood drawn from animals at three time points in an ovine model of silicon-doped diamond-like carbon–coated implantation in tibia. (a) superoxide generation; (b) NO generation; (c) arginase-1 activity; (d) urea/NO ratio. NS – non-stimulated macrophages; PPP – platelet-poor plasma–stimulated macrophages; P-PRP – macrophages stimulated with pure platelet-rich plasma with no or a low number of leukocytes; L-PRP – macrophages stimulated with platelet-rich plasma with a high number of leukocytes; T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation; * – significant difference to NS at each time point (P-value < 0.05)
Comparison of the effect of blood-derived product stimulation on macrophage response in blood drawn from animals at three time points in an ovine model of silicon-doped diamond-like carbon–coated implantation in tibia. (a) superoxide generation; (b) NO generation; (c) arginase-1 activity; (d) urea/NO ratio. NS – non-stimulated macrophages; PPP – platelet-poor plasma–stimulated macrophages; P-PRP – macrophages stimulated with pure platelet-rich plasma with no or a low number of leukocytes; L-PRP – macrophages stimulated with platelet-rich plasma with a high number of leukocytes; T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation; * – significant difference to NS at each time point (P-value < 0.05)

Fig. 3.

Representative microphotographs of morphological changes in macrophages in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. Optical microscopy ×40 carried out 48 h after stimulation with different blood-derived products at three time points. A – naïve macrophages after DMEM and 10% BCF supplementation; B, D – rounded cells visible in all cultures after platelet-poor plasma (PPP) stimulation; C, E–H – pseudopodia (blue arrows) induced after stimulation by platelet-rich plasma with a high number of leukocytes (L-PRP) or with platelet-rich plasma with no or a low number of leukocytes (P-PRP); T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation
Representative microphotographs of morphological changes in macrophages in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. Optical microscopy ×40 carried out 48 h after stimulation with different blood-derived products at three time points. A – naïve macrophages after DMEM and 10% BCF supplementation; B, D – rounded cells visible in all cultures after platelet-poor plasma (PPP) stimulation; C, E–H – pseudopodia (blue arrows) induced after stimulation by platelet-rich plasma with a high number of leukocytes (L-PRP) or with platelet-rich plasma with no or a low number of leukocytes (P-PRP); T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation
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