Login
Registrieren
Passwort zurücksetzen
Veröffentlichen & Verteilen
Verlagslösungen
Vertriebslösungen
Themen
Allgemein
Altertumswissenschaften
Architektur und Design
Bibliotheks- und Informationswissenschaft, Buchwissenschaft
Biologie
Chemie
Geowissenschaften
Geschichte
Industrielle Chemie
Informatik
Jüdische Studien
Kulturwissenschaften
Kunst
Linguistik und Semiotik
Literaturwissenschaft
Materialwissenschaft
Mathematik
Medizin
Musik
Pharmazie
Philosophie
Physik
Rechtswissenschaften
Sozialwissenschaften
Sport und Freizeit
Technik
Theologie und Religion
Wirtschaftswissenschaften
Veröffentlichungen
Zeitschriften
Bücher
Konferenzberichte
Verlage
Blog
Kontakt
Suche
EUR
USD
GBP
Deutsch
English
Deutsch
Polski
Español
Français
Italiano
Warenkorb
Home
Zeitschriften
Journal of Veterinary Research
Band 68 (2024): Heft 1 (March 2024)
Uneingeschränkter Zugang
The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon
Bartłomiej Szymczak
Bartłomiej Szymczak
,
Andrzej Junkuszew
Andrzej Junkuszew
,
Krzysztof Patkowski
Krzysztof Patkowski
,
Tomasz Szponder
Tomasz Szponder
,
Dominika Nguyen Ngoc
Dominika Nguyen Ngoc
,
Beata Drzewiecka
Beata Drzewiecka
,
Aleksandra Sobczyńska-Rak
Aleksandra Sobczyńska-Rak
und
Joanna Wessely-Szponder
Joanna Wessely-Szponder
| 23. März 2024
Journal of Veterinary Research
Band 68 (2024): Heft 1 (March 2024)
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zusammenfassung
Artikel
Figuren und Tabellen
Referenzen
Autoren
Artikel in dieser Ausgabe
Vorschau
PDF
Zitieren
Teilen
Online veröffentlicht:
23. März 2024
Seitenbereich:
167 - 174
Eingereicht:
28. Juli 2023
Akzeptiert:
15. Jan. 2024
DOI:
https://doi.org/10.2478/jvetres-2024-0003
Schlüsselwörter
blood-derived products
,
macrophages
,
Si-DLC implant
,
ovine model
© 2024 Bartłomiej Szymczak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1.
Diagram of the experimental design for evaluating monocyte-derived macrophage (MDM) activity in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. T – time point; PBMC – peripheral blood mononuclear cells; PPP – platelet-poor plasma; P-PRP – pure platelet-rich plasma with no or a low number of leukocytes; L-PRP –platelet-rich plasma with a high number of leukocytes
Fig. 2.
Comparison of the effect of blood-derived product stimulation on macrophage response in blood drawn from animals at three time points in an ovine model of silicon-doped diamond-like carbon–coated implantation in tibia. (a) superoxide generation; (b) NO generation; (c) arginase-1 activity; (d) urea/NO ratio. NS – non-stimulated macrophages; PPP – platelet-poor plasma–stimulated macrophages; P-PRP – macrophages stimulated with pure platelet-rich plasma with no or a low number of leukocytes; L-PRP – macrophages stimulated with platelet-rich plasma with a high number of leukocytes; T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation; * – significant difference to NS at each time point (P-value < 0.05)
Fig. 3.
Representative microphotographs of morphological changes in macrophages in blood drawn from animals in an ovine model of silicon-doped diamond-like carbon–coated (Si-DLC) implantation in tibia. Optical microscopy ×40 carried out 48 h after stimulation with different blood-derived products at three time points. A – naïve macrophages after DMEM and 10% BCF supplementation; B, D – rounded cells visible in all cultures after platelet-poor plasma (PPP) stimulation; C, E–H – pseudopodia (blue arrows) induced after stimulation by platelet-rich plasma with a high number of leukocytes (L-PRP) or with platelet-rich plasma with no or a low number of leukocytes (P-PRP); T0 – time point before implantation; T1 – four weeks after implantation; T2 – four months after implantation