<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental stimulation. Their functional phenotype can be affected by platelet-derived products as determined by those products’ composition. When the inflammatory response caused by implantation is excessive, it can lead to rejection of the implant. Therefore, a thorough evaluation of implant haemocompatibility is necessary to minimise undesirable consequences.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>In an <italic>in vitro</italic> study, monocyte-derived macrophages (MDMs) were obtained from the whole blood of sheep after a silicon-doped diamond-like carbon–coated implant insertion. These MDMs were then exposed to autologous platelet-derived products for functional marker analysis.</p>
</sec>
<sec>
<h3>Results</h3>
<p>Platelet-poor plasma (PPP) and pure platelet-rich plasma (P-PRP) stimulation increased arginase-1 activity, while leukocyte-rich PRP stimulation produced a mixed response involving higher O<sub>2</sub><sup>−</sup> (6.49 ± 2.43 nM <italic>vs</italic> non-stimulated 3.51 ± 1.23 nM, P-value &lt; 0.05) and NO (3.28 ± 1.38 μM <italic>vs</italic> non-stimulated 2.55 ± 0.32μM, P-value &lt; 0.05) generation.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>Using PPP and P-PRP stimulation in post-implantation procedures may contribute to the polarisation of macrophages towards the M2-like pro-resolving phenotype, thereby accelerating wound healing. This would also prevent implant degradation due to an excessive inflammatory process.</p>
</sec>
</abstract>

Introduction
Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental stimulation. Their functional phenotype can be affected by platelet-derived products as determined by those products’ composition. When the inflammatory response caused by implantation is excessive, it can lead to rejection of the implant. Therefore, a thorough evaluation of implant haemocompatibility is necessary to minimise undesirable consequences.


Material and Methods
In an in vitro study, monocyte-derived macrophages (MDMs) were obtained from the whole blood of sheep after a silicon-doped diamond-like carbon–coated implant insertion. These MDMs were then exposed to autologous platelet-derived products for functional marker analysis.


Results
Platelet-poor plasma (PPP) and pure platelet-rich plasma (P-PRP) stimulation increased arginase-1 activity, while leukocyte-rich PRP stimulation produced a mixed response involving higher O2− (6.49 ± 2.43 nM vs non-stimulated 3.51 ± 1.23 nM, P-value < 0.05) and NO (3.28 ± 1.38 μM vs non-stimulated 2.55 ± 0.32μM, P-value < 0.05) generation.


Conclusion
Using PPP and P-PRP stimulation in post-implantation procedures may contribute to the polarisation of macrophages towards the M2-like pro-resolving phenotype, thereby accelerating wound healing. This would also prevent implant degradation due to an excessive inflammatory process.

The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental...

The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon

Published Online: Mar 23, 2024

Page range: 167 - 174

Received: Jul 28, 2023

Accepted: Jan 15, 2024

DOI: https://doi.org/10.2478/jvetres-2024-0003

Keywords
blood-derived products, macrophages, Si-DLC implant, ovine model

© 2024 Bartłomiej Szymczak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon

Published Online: Mar 23, 2024

Page range: 167 - 174

Received: Jul 28, 2023

Accepted: Jan 15, 2024

DOI: https://doi.org/10.2478/jvetres-2024-0003

Keywordsblood-derived products, macrophages, Si-DLC implant, ovine model

© 2024 Bartłomiej Szymczak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
blood-derived products, macrophages, Si-DLC implant, ovine model