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Immunoreactivity of p21, MMP-1 and CB2 receptor proteins in cutaneous canine mast cell tumours: an association with the three-tier grading system

Kamila Bulak
Kamila Bulak
, 
Anna Kycko
Anna Kycko
, 
Anna Śmiech
Anna Śmiech
 and 
Wojciech Łopuszyński
Wojciech Łopuszyński
   | Dec 19, 2023
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Published Online: Dec 19, 2023
Page range: 611 - 618
Received: Jul 22, 2023
Accepted: Nov 22, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0066
Keywords
© 2023 Kamila Bulak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Staining of mast cell tumours (MCTs). A – Haematoxylin & eosin (H&E) staining visualising a high degree of cell differentiation in a grade 1 MCT; B – H&E staining visualising an intermediate degree of cell differentiation in a grade 2 MCT; C – H&E staining visualising poorly differentiated neoplastic cells in a grade 3 MCT; D – Intense toluidine blue staining visualising intracytoplasmic granules of neoplastic mast cells in a grade 1 MCT; E – Toluidine blue staining visualising individual cells in a grade 2 MCT; F – Weak toluidine blue staining visualising a grade 3 MCT
Staining of mast cell tumours (MCTs). A – Haematoxylin & eosin (H&E) staining visualising a high degree of cell differentiation in a grade 1 MCT; B – H&E staining visualising an intermediate degree of cell differentiation in a grade 2 MCT; C – H&E staining visualising poorly differentiated neoplastic cells in a grade 3 MCT; D – Intense toluidine blue staining visualising intracytoplasmic granules of neoplastic mast cells in a grade 1 MCT; E – Toluidine blue staining visualising individual cells in a grade 2 MCT; F – Weak toluidine blue staining visualising a grade 3 MCT

Fig. 2.

Frequency of immunoreactivity for matrix metalloproteinase 1 (MMP-1) receptor protein in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; **– P-value < 0.01; ***– P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction
Frequency of immunoreactivity for matrix metalloproteinase 1 (MMP-1) receptor protein in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; **– P-value < 0.01; ***– P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction

Fig. 3.

Immunohistochemistry with anti-matrix metalloproteinase 1 (MMP-1) primary antibody with positive reaction visible as brown staining. A – strong cytoplasmic reaction in a grade 1 mast cell tumours (MCTs); B – moderate cytoplasmic reaction in a grade 2 MCT; C – weak cytoplasmic reaction in a grade 3 MCT
Immunohistochemistry with anti-matrix metalloproteinase 1 (MMP-1) primary antibody with positive reaction visible as brown staining. A – strong cytoplasmic reaction in a grade 1 mast cell tumours (MCTs); B – moderate cytoplasmic reaction in a grade 2 MCT; C – weak cytoplasmic reaction in a grade 3 MCT

Fig. 4.

Frequency of immunoreactivity for p21 protein in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; **_ P-value < 0.01; *** P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction
Frequency of immunoreactivity for p21 protein in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; **_ P-value < 0.01; *** P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction

Fig. 5.

Immunohistochemistry with primary anti-p21 antibody with positive reaction visible as brown staining. A – weak nuclear reaction in a grade 1 mast cell tumour (MCT); B – moderate nuclear reaction in a grade 2 MCT; C – strong nuclear reaction in a grade 3 MCT
Immunohistochemistry with primary anti-p21 antibody with positive reaction visible as brown staining. A – weak nuclear reaction in a grade 1 mast cell tumour (MCT); B – moderate nuclear reaction in a grade 2 MCT; C – strong nuclear reaction in a grade 3 MCT

Fig. 6.

Frequency of immunoreactivity for cannabinoid type 2 receptor proteins in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction
Frequency of immunoreactivity for cannabinoid type 2 receptor proteins in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction

Fig. 7.

Immunohistochemistry with primary anti–cannabinoid type2 receptor antibody with positive reaction visible as brown staining. A – moderate to strong cytoplasmic reaction in a grade 1 mast cell tumour (MCT); B – moderate cytoplasmic reaction in a grade 2 MCT; C – weak cytoplasmic reaction in a grade 3 MCT
Immunohistochemistry with primary anti–cannabinoid type2 receptor antibody with positive reaction visible as brown staining. A – moderate to strong cytoplasmic reaction in a grade 1 mast cell tumour (MCT); B – moderate cytoplasmic reaction in a grade 2 MCT; C – weak cytoplasmic reaction in a grade 3 MCT
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