Mast cell tumours (MCTs) have been characterised as the effect of a haematopoietic disorder affecting both humans and animals and resulting in the malignant transformation of mast cells of various organs (28). These tumours arise predominantly in the dermis and subcutaneous tissues, and in dogs, they are frequently malignant and are the most common form of skin neoplasm (27). This type of neoplasm provides good material for research in the areas of carcinogenesis and anti-cancer treatment because of its high incidence in dogs.
One of the widely explored therapeutic options is the use of cannabinoids. The presence of cannabinoid receptors in the skin has been demonstrated in animals and humans; therefore, one of the potential therapeutic targets in MCT research might be the endocannabinoid system (4). According to various studies, phytocannabinoids derived from
One of the apoptosis-related proteins which have been extensively investigated in the context of anti-cancer therapy is the cyclin-dependent kinase inhibitor (p21). Scientific research has proved that the p21 protein is characterised by functional duality and that its ability to inhibit apoptosis results in an oncogenic effect (18). Increased activity of the p21 protein makes cells more susceptible to the ageing process, which in turn may contribute to the growth of unfavourable mutations and, consequently, to neoplastic transformation (1, 5). The p21 protein protects cells from excessive apoptosis caused by chemotherapeutic drugs. Reducing the expression of the gene encoding the p21 protein increases the sensitivity of cancer cells to chemotherapy (12).
The extracellular matrix is present in all tissues as an amorphous structure with a unique composition. In order to maintain homeostasis, it is constantly remodelled by matrix metalloproteinases (MMPs) (3). These proteinases are characterised by low activity in physiological conditions and high activity in pathologically changed or inflamed tissues. Cytokines, growth factors and stimulants from neoplastic cells are responsible for the increase in MMP activity (25). In tumour development, the degradation of collagen as a result of the MMP-1 activity promotes cell migration. Moreover, MMPs have the potential to stimulate angiogenesis, and thus to spread neoplastic cells
The current knowledge on p21, MMP-1 and CB2 immunoreactivity in dogs is limited and based on few studies. These three proteins are considered potential targets for novel anti-tumour therapeutic strategies. The aim of this study was to evaluate by immunohistochemical means the reactivity of the p21, MMP-1 and CB2R proteins in association with a three-tier grading system in cutaneous canine MCTs. Our results were compared with those of previously published studies and a literature review was performed.
The material for the study involved a selection of archival formalin-fixed, paraffin-embedded tissues from solitary canine cutaneous MCTs removed surgically with clear margins. A selection of 15 tumour samples with a grade 1 MCT, 15 tumour samples with a grade 2 MCT and 15 tumour samples with a grade 3 MCT was made for subsequent immunohistochemical analyses. The tissue sections were stained with haematoxylin and eosin for histopathology. In order to visualise the metachromatic cytoplasmic granules, a routine toluidine blue staining was used as an additional method to identify mast cells (22).
The grade of MCT malignancy was assessed according to the criteria of Patnaik
For immunohistochemistry (IHC), 4-μm-thick tissue sections were attached to SuperFrost Plus slides (Thermo Fisher Scientific, Waltham, MA, USA). After deparaffinisation and rehydration, endogenous peroxidase activity was quenched with a peroxidase suppressor for 10 min. Heat-induced antigen retrieval was performed in a water bath for 30 min using an ethylenediaminetetraacetic acid buffer (pH 8.0) to expose the CB2 receptor, p21 and MMP-1 proteins. Next, the tissues were permeabilised in 3% bovine serum albumin–phosphate-buffered saline (PBS) solution for 30 min at room temperature, then incubated with primary antibodies for 1 h at room temperature in a humidified chamber. The primary antibodies used in this study, all canine-specific, were as follows: anti-CB2R (Cat. No. TA317640; OriGene Technologies, Rockville, MD, USA), anti-p21 (Cat. No. M7202; clone SX118, Agilent Dako, Santa Clara, CA, USA) and anti-MMP-1 (Cat. No. ABIN2777120; antibodies-online, Aachen, Germany), diluted 1 : 200, 1 : 50 and 1 : 500, respectively. Tris-buffered saline, (Sigma-Aldrich, St. Louis, MO, USA) was used instead of the primary antibody to obtain the negative control for the IHC test. Tissue sections were then washed in PBST (0.1% Tween 20 in PBS solution), and the IHC reaction was carried out by an indirect method using an UltraVision Quanto Detection System (Cat. No. TL-060-QHD; Thermo Fisher Scientific, Waltham, MA, USA) with 3,3′-diaminobenzidine as the chromogen substrate to mark the reaction sites. Tissues were counterstained with haematoxylin (Novocastra; Leica Biosystems, Deer Park, IL, USA) and prepared for mounting. Microscopic slides were examined using an Eclipse E600 light microscope (Nikon Instruments, Tokyo, Japan). Photographs were taken with a digital camera (Nikon DS-Fi1; Nikon Instruments, Tokyo, Japan).
The number of positive cells in the immunohistochemical reaction and the total stained area were determined semi-quantitatively. Marker immunoreactivity was examined only for mast cells, as under a light microscope, these cells can be easily distinguished from other inflammatory cells (such as eosinophils) based on morphology and size. Marker immunoreactivity was measured above a fixed threshold applied to all images to distinguish specific staining from background using NIS-Elements BR-2.20 imaging software (Nikon Europe, Prague, Czech Republic). The stained cells were categorised into 0 (negative), + (weakly positive), ++ (moderately positive), and +++ (strongly positive) groups. Three different experienced pathologist observers manually quantified each image. Positive or negative status for CB2R/p21/MMP-1 was assessed in three random fields of view (400× HPF, 2.37 mm2), and the total percentage score was obtained after evaluating at least 900 neoplastic mast cells in each tumour.
The statistical analysis was carried out in IBM SPSS Statistics 28.0 software (IBM, Armonk, NY, USA). The associations were evaluated through Pearson’s χ2 test. Subsequently, Fisher’s
The histopathological examination divided the MCTs into three groups displaying three levels of cell-differentiation: grade 1 tumours showed a high degree of cell differentiation, grade 2 tumours had an intermediate degree of cell differentiation, and grade 3 tumours were characterised by a low degree of differentiation with distinct cellular atypia and a tendency to form multinucleated cells (Fig. 1 A–C). Grade 1 tumours were stained intensely with toluidine blue, while with increasing tumour grade, the intensity of the staining was weaker and the granular character of the cytoplasm was less visible (Fig. 1 D–F).
Staining of mast cell tumours (MCTs). A – Haematoxylin & eosin (H&E) staining visualising a high degree of cell differentiation in a grade 1 MCT; B – H&E staining visualising an intermediate degree of cell differentiation in a grade 2 MCT; C – H&E staining visualising poorly differentiated neoplastic cells in a grade 3 MCT; D – Intense toluidine blue staining visualising intracytoplasmic granules of neoplastic mast cells in a grade 1 MCT; E – Toluidine blue staining visualising individual cells in a grade 2 MCT; F – Weak toluidine blue staining visualising a grade 3 MCT
Immunoreactivity for the MMP-1 protein was denoted by brown cytoplasmic staining. The intensity of the immunohistochemical reaction was strong in grade 1, moderate in grade 2 and weak in grade 3 tumours (Fig. 3 A–C). The immunolabelling of p21, visible in the nuclei, was the strongest in grade 3 tumours and gradually weaker with increasing tumour differentiation in lower-grade tumours (Fig. 5 A–C). The cytoplasmic immunohistochemical reaction for CB2R protein was marked in grade 1 tumours and decreased with increasing grade of the tumours (Fig. 7 A–C). There was a significant association (strong for MMP-1 and p21, and moderate for CB2R) between the intensity of the reaction and the three-tier grading system (P-value <0.001). Grade 1 MCTs showed mild or no detectable p21 immunoreactivity (P-value <0.001). In contrast to p21, MMP-1 and CB2 displayed marked or moderate immunoreactivity in grade 1 and grade 2 tumours (Figs 2, 4 and 6). Strong reaction intensity for MMP-1 was more common in grade 1 tumours (P-value <0.001) and for CB2R was significantly less frequent in grade 3 tumours than in grade 1 and grade 2 tumours (P-value <0.001) (Fig. 6). In grade 2 tumours strong immunoreactivity for the MMP-1 protein was as frequent as for the CB2R protein (P-value = 1.000).
Frequency of immunoreactivity for matrix metalloproteinase 1 (MMP-1) receptor protein in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; **– P-value < 0.01; ***– P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction
Immunohistochemistry with anti-matrix metalloproteinase 1 (MMP-1) primary antibody with positive reaction visible as brown staining. A – strong cytoplasmic reaction in a grade 1 mast cell tumours (MCTs); B – moderate cytoplasmic reaction in a grade 2 MCT; C – weak cytoplasmic reaction in a grade 3 MCT
Frequency of immunoreactivity for p21 protein in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; **_ P-value < 0.01; *** P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction
Immunohistochemistry with primary anti-p21 antibody with positive reaction visible as brown staining. A – weak nuclear reaction in a grade 1 mast cell tumour (MCT); B – moderate nuclear reaction in a grade 2 MCT; C – strong nuclear reaction in a grade 3 MCT
Frequency of immunoreactivity for cannabinoid type 2 receptor proteins in grade 1, 2 and 3 mast cell tumours (MCTs). * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001 compared to grade 1 MCTs; (−) – no reaction; (+) – weak reaction; (++) – moderate reaction; (+++) – strong reaction
Immunohistochemistry with primary anti–cannabinoid type2 receptor antibody with positive reaction visible as brown staining. A – moderate to strong cytoplasmic reaction in a grade 1 mast cell tumour (MCT); B – moderate cytoplasmic reaction in a grade 2 MCT; C – weak cytoplasmic reaction in a grade 3 MCT
The skin, as well as the mucous membranes of the respiratory tract, digestive tract and genitourinary organs, are particularly rich in mast cells (28). Besides immune modulation and presentation of antigens, participation in angiogenesis and tissue remodelling are also functions of mast cells (2, 26). The effect of a wide spectrum of stimuli affecting mast cells may be the excessive and uncontrolled growth of mast cells, referred to as mast cell tumours. Tumours of this kind account for 16–21% of skin neoplasms in dogs (27). Macroscopically, MCTs are often confused with and misdiagnosed as oedema, inflammation or non-neoplastic lesions, leading to inappropriate treatment regimens and impacting prognosis negatively. In the tumour microenvironment, excessive production of chemokines by malignant mast cells induces stimulation of eosinophils, resulting in peripheral eosinophilia. In turn, the stem cell factor released by eosinophils supports further activation of mast cells. The paraneoplastic hypereosinophilia which accompanies MCTs positively correlates with higher recurrence rates (11). Eosinophils have the ability to secrete pro-inflammatory tissue-degrading mediators, including collagenases that facilitate the degradation of type I, II and III collagen. The immunoreactivity of MMPs in eosinophils has not been detected, but a positive correlation between the number of eosinophils and the degree of degraded type I collagen has been proved (20). In our study, MMP-1 was detected in the cytoplasm of the cells comprising the neoplastic cellular masses. This proteinase, also termed collagenase-1, has been described to be mainly expressed by fibroblasts, while increased expression of MMP-1 has been reported in various inflammatory and neoplastic diseases (13). Di Girolamo
Expression of the p21 protein in the examined tumours was detected in the nuclei of the neoplastic cells. The comparative analysis revealed that the p21 protein showed significantly higher (P-value <0.001) immunoreactivity in the grade 3 tumours compared to the well-differentiated ones. Similar results were obtained by Wu
The immunohistochemical analysis of the CB2 receptor in the examined MCTs revealed the significantly higher (P-value <0.001) CB2R immunoreactivity in grade 1 and grade 2 tumours. The results are consistent with those in the recent report by Rinaldi
By reviewing the literature data, it can be concluded that there is little research so far that includes the immunoreactivity of markers (p21, MMP-1 and CB2R) in mast cell tumours in dogs. The results of our study attribute high immunoreactivity to MMP-1 protein and p21 protein in MCTs, and suggest that MMP-1 can be a marker of grade 1 and p21 of grade 3 canine MCTs. Moreover, the positive immunoreactivity of CB2R with the simultaneous low or absent immunoreactivity of p21 and high immunoreactivity of MMP-1 may be considered an indicator to encourage the usage of cannabinoids in grade 1 MCTs in dogs. Nuclear p21 immunoreactivity and cytoplasmic MMP-1/CB2R immunoreactivity in canine cutaneous MCTs require more case studies based on breed, the presence or absence of metastases, the degree of ulceration, and the primary tumour location. Our study demonstrates nevertheless that the applied method of comparing histological evidence of immunoreactivity with tumour grading can be used for analyses in the future, when the Patnaik and Kiupel MCT grading systems should also be compared because canine cutaneous MCTs are currently classified using both. Moreover, the assessment of the immunoreactivity of MMP-1 and p21 proteins in MCTs may have prognostic significance in the future in clinical trials in the field of veterinary oncology. The estimation of CB2R expression together with monitoring of other tumour markers could enable precise treatment regimens to be designed and improve the quality of life of animals with MCTs through the early introduction of cannabinoids. The limitation of the study was the availability of only formalin-fixed, paraffin-embedded archival tissues to assess the expression of the selected protein markers. An investigation of possible relations between MMP-1, p-21 and CB2R in canine MCTs would require a genomic and transcriptomic approach relying on fresh biological material. Nevertheless, this work may have brought attention to a field for further research in the context of comparative oncology of MCTs in animals and humans. Since mast cell tumours are more common in animals than in humans, animal tissue has the potential to become an MCT research model in humans.