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Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China

Wang Xifeng

Wang Xifeng

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Xifeng, Wang
, 
Zhang Kai

Zhang Kai

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Kai, Zhang
, 
Zhang Guowu

Zhang Guowu

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Guowu, Zhang
, 
Li Zhiyuan

Li Zhiyuan

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Zhiyuan, Li
, 
Shang Yunxia

Shang Yunxia

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Yunxia, Shang
, 
Ning Chengcheng

Ning Chengcheng

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Chengcheng, Ning
, 
Ji Chunhui

Ji Chunhui

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Chunhui, Ji
, 
Qiao Jun

Qiao Jun

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Jun, Qiao
, 
Meng Qingling

Meng Qingling

College of Animal Science & Technology, Shihezi University, ShiheziXinjiang, PR China
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Qingling, Meng
 and 
Cai Xuepeng

Cai Xuepeng

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, LanzhouGansu, PR China
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Xuepeng, Cai
  
Apr 22, 2022
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Published Online: Apr 22, 2022
Page range: 199 - 207
Received: Sep 14, 2021
Accepted: Mar 18, 2022
DOI: https://doi.org/10.2478/jvetres-2022-0018
Keywords
© 2022 W. Xifeng et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Fasciola hepatica is a trematode infecting ruminants worldwide and occasionally affecting other animal species, including humans. It causes significant economic losses. Geographic distribution and patterns of infection must be considered before control and management measures are developed for this parasite. DNA molecular markers are useful for the identification of flukes and elucidation of their genetic evolution. Therefore, the population structure of F. hepatica was studied using this method in sheep in Xinjiang, China.

Material and Methods

The molecular characteristics, genetic relationships within the population and dispersal patterns of F. hepatica isolates were analysed based on the cox1 and nad1 genes. The population structure of F. hepatica from three regions of Xinjiang was explored and a neutrality test was conducted.

Results

The cox1 and nad1 genes have 21 and 42 variable sites, respectively, which can be classified into 34 and 33 haplotypes. Median-joining network and phylogenetic tree analyses showed that there was no significant variation in F. hepatica isolates between the three geographical regions. Analysis of variance revealed that the genetic variation of F. hepatica was mainly present within the populations. The neutrality test indicated that the populations were relatively stable but the Hami population may have undergone short-term expansion.

Conclusion

This study revealed for the first time the molecular characteristics, genetic diversity and dispersal patterns of F. hepatica isolates from sheep in Xinjiang, thus providing new insights into the genetic variation and haplotype diversity of F. hepatica from indigenous sheep.
Language:
English
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Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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