Open Access

Characterisation and antibiotic susceptibility profile of Clostridioides (Clostridium) difficile isolated from chicken carcasses

Enver Baris Bingol

Enver Baris Bingol

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
Search for this author on
Sciendo|Google Scholar
Bingol, Enver Baris
, 
Hamparsun Hampikyan

Hamparsun Hampikyan

Faculty of Fine Arts, Department of Gastronomy and Culinary ArtsIstanbul, Turkey
Search for this author on
Sciendo|Google Scholar
Hampikyan, Hamparsun
, 
Karlo Muratoglu

Karlo Muratoglu

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
Search for this author on
Sciendo|Google Scholar
Muratoglu, Karlo
, 
Esra Akkaya

Esra Akkaya

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
Search for this author on
Sciendo|Google Scholar
Akkaya, Esra
, 
Omer Cetin

Omer Cetin

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
Search for this author on
Sciendo|Google Scholar
Cetin, Omer
 and 
Hilal Colak

Hilal Colak

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
Search for this author on
Sciendo|Google Scholar
Colak, Hilal
  
Aug 11, 2020
Characterisation and antibiotic susceptibility profile of Clostridioides (Clostridium) difficile isolated from chicken carcasses's Cover Image
About this article
Previous Article
Next Article
Cite
Download Cover
Published Online: Aug 11, 2020
Page range: 407 - 412
Received: Jan 16, 2020
Accepted: Jul 27, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0052
Keywords
© 2020 E.B. Bingol et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Clostridioides (Clostridium) difficile is a Gram+, anaerobic, spore-forming, rod-shaped bacterium that can produce toxins, and it is mainly because its virulence is attributed. The objective of this study was to evaluate the presence of C. difficile and hyper virulent ribotypes in chicken carcasses and the antibiotic susceptibility of isolated strains.

Material and Methods

C. difficile was isolated from chicken carcasses by microbiological methods, its ribotypes were identified by means of PCR, the toxin production ability was defined by ELISA, and the susceptibility of the isolates to selected antibiotics was determined by minimum inhibitory concentration evaluator strips.

Results

The bacterium was isolated from 69 out of 185 (37.3%) examined chicken carcass samples, and six out of the 69 (8.7%) isolates were identified as ribotype 027. All isolates were susceptible to amoxicillin-clavulanic acid (100.0%), vancomycin (97.1%), metronidazole (88.4%), and tetracycline (95.7%), whereas they were resistant to cefotaxime (97.1%) and imipenem (89.9%).

Conclusion

The results of this study demonstrate the presence of toxigenic C. difficile isolates such as ribotype 027 (one of the most common causes of C. difficile infection in humans) in chicken carcasses. Although there is no case for stating that C. difficile is a food-borne pathogen, the presence of C. difficile in chicken may be considered to be a potential risk to consumers.
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
Journal RSS Feed