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Characterisation and antibiotic susceptibility profile of Clostridioides (Clostridium) difficile isolated from chicken carcasses

Enver Baris Bingol

Enver Baris Bingol

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
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Bingol, Enver Baris
, 
Hamparsun Hampikyan

Hamparsun Hampikyan

Faculty of Fine Arts, Department of Gastronomy and Culinary ArtsIstanbul, Turkey
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Hampikyan, Hamparsun
, 
Karlo Muratoglu

Karlo Muratoglu

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
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Muratoglu, Karlo
, 
Esra Akkaya

Esra Akkaya

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
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Akkaya, Esra
, 
Omer Cetin

Omer Cetin

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
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Cetin, Omer
 und 
Hilal Colak

Hilal Colak

Department of Food Hygiene and Technology, Faculty of Veterinary MedicineIstanbul, Turkey
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Colak, Hilal
  
11. Aug. 2020
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Online veröffentlicht: 11. Aug. 2020
Seitenbereich: 407 - 412
Eingereicht: 16. Jan. 2020
Akzeptiert: 27. Juli 2020
DOI: https://doi.org/10.2478/jvetres-2020-0052
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© 2020 E.B. Bingol et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Clostridioides (Clostridium) difficile is a Gram+, anaerobic, spore-forming, rod-shaped bacterium that can produce toxins, and it is mainly because its virulence is attributed. The objective of this study was to evaluate the presence of C. difficile and hyper virulent ribotypes in chicken carcasses and the antibiotic susceptibility of isolated strains.

Material and Methods

C. difficile was isolated from chicken carcasses by microbiological methods, its ribotypes were identified by means of PCR, the toxin production ability was defined by ELISA, and the susceptibility of the isolates to selected antibiotics was determined by minimum inhibitory concentration evaluator strips.

Results

The bacterium was isolated from 69 out of 185 (37.3%) examined chicken carcass samples, and six out of the 69 (8.7%) isolates were identified as ribotype 027. All isolates were susceptible to amoxicillin-clavulanic acid (100.0%), vancomycin (97.1%), metronidazole (88.4%), and tetracycline (95.7%), whereas they were resistant to cefotaxime (97.1%) and imipenem (89.9%).

Conclusion

The results of this study demonstrate the presence of toxigenic C. difficile isolates such as ribotype 027 (one of the most common causes of C. difficile infection in humans) in chicken carcasses. Although there is no case for stating that C. difficile is a food-borne pathogen, the presence of C. difficile in chicken may be considered to be a potential risk to consumers.
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Biologie, Molekularbiologie, Mikrobiologie und Virologie, Biologie, andere, Medizin, Veterinärmedizin
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