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Biosensor of inflammation biomarkers based on electrical bioimpedance analysis on immobilized DNA without chemical modification

Modesto Gómez-López

Modesto Gómez-López

Escuela Superior de Medicina, Instituto Politécnico NacionalCDMX, México
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Gómez-López, Modesto
, 
Ángel Miliar-García

Ángel Miliar-García

Escuela Superior de Medicina, Instituto Politécnico NacionalCDMX, México
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Miliar-García, Ángel
, 
Nadia Mabel Pérez-Vielma

Nadia Mabel Pérez-Vielma

Centro Interdisciplinario de Ciencias de la Salud-Unidad Santo Tomás, Instituto Politécnico NacionalCDMX, México
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Pérez-Vielma, Nadia Mabel
, 
Eleazar Lara-Padilla

Eleazar Lara-Padilla

Escuela Superior de Medicina, Instituto Politécnico NacionalCDMX, México
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Lara-Padilla, Eleazar
 and 
César Antonio González-Díaz

César Antonio González-Díaz

Escuela Superior de Medicina, Instituto Politécnico NacionalCDMX, México
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González-Díaz, César Antonio
  
Jun 25, 2020
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Published Online: Jun 25, 2020
Page range: 31 - 37
Received: Mar 10, 2020
DOI: https://doi.org/10.2478/joeb-2020-0006
Keywords
© 2020 Modesto Gómez-López et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Components of the cDNA biosensor system.
Components of the cDNA biosensor system.

Fig. 2

Diagram of functionalized gold surface quartz crystal and representation of the cDNA anchorage and hybridization.
Diagram of functionalized gold surface quartz crystal and representation of the cDNA anchorage and hybridization.

Fig. 3

Nyquiste plot EBiS spectra for the three genes studied (NLRP3, IL-1B and Caspase 1) as well as control (vehicle) and negative control (non-specific gene) conditions.
Nyquiste plot EBiS spectra for the three genes studied (NLRP3, IL-1B and Caspase 1) as well as control (vehicle) and negative control (non-specific gene) conditions.

Fig. 4

Relative integrated EBiS vs relative qPCR expression of the genes NLRP3, IL-1β, and Casp-1. One-way ANOVA test *p<0.01, **p<0.05.
Relative integrated EBiS vs relative qPCR expression of the genes NLRP3, IL-1β, and Casp-1. One-way ANOVA test *p<0.01, **p<0.05.

Fig. 5

Numerical modelling of a simple DNA molecule anchoring on an electrode surface. Frequency response of the RC equivalent circuit calculated by LTspice IV.
Numerical modelling of a simple DNA molecule anchoring on an electrode surface. Frequency response of the RC equivalent circuit calculated by LTspice IV.
Language:
English
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1 times per year
Journal Subjects:
Engineering, Bioengineering and Biomedical Engineering, Biomedical Electronics, Life Sciences, Biophysics, Medicine, Biomedical Engineering, Physics, Spectroscopy and Metrology
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