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Fig. 1
Map of sampled locations. PR: Pinar del Rio, LH: La Habana, MY: Mayabeque, MT: Matanzas, LT: Las Tunas, HO: Holguín, SC: Santiago de Cuba, GT: Guantánamo. Black triangles represent localization on the sampled apiaries.
Fig. 2
Restriction profiles of COI region of Varroa destructor mtDNA. Eight provinces of Cuba were sampled and displayed numbered from 1 to 8. 1: PR, 2: LH, 3: MY, 4: MT, 5: LT, 6: HO, 7: SC and 8:GT. The mtDNA was digested with endonucleases SacI and EcoNI. Lane L: 100-bp DNA ladder, Lane A: Undigested amplicons (amplified with COXF and COXRa primers) and Lane B: Undigested amplicons (amplified with VD-CO206F and VD-CO206R).
Microsatellites absolute sizes and frequencies of alleles found in Cuba. He and Ho are expected and observed heterocigocity, respectively