<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Varroa destructor</italic> has been present in Cuba since 1996, but without the use of acaricidal infestation rates remain at very low levels. The presence of Korean haplotype mites was described in 2007, but there is no information regarding the introgression of the less virulent Japanese haplotype that could account for a low pathogenicity of the mite. In this research, we carried out molecular characterization of Cuban Varroa mites through mitochondrial DNA and hypervariable nuclear loci. We applied an alternative RFLP technique and found that all the analyzed samples corresponded to Korean haplotypes. We analyzed the three STRs loci VD112, VD114 and VD016, previously described as highly variable and found new alleles in all of them, with an absolute allele size very different to those reported worldwide. We also detected genic and genotypic differentiation between samples from two nearby locations (P=0.08). We also tested a new RFLP method for mite haplotype discrimination with an intra-reaction positive control of digestion.</p></abstract>

Varroa destructor has been present in Cuba since 1996, but without the use of acaricidal infestation rates remain at very low levels. The presence of Korean haplotype mites was described in 2007, but there is no information regarding the introgression of the less virulent Japanese haplotype that could account for a low pathogenicity of the mite. In this research, we carried out molecular characterization of Cuban Varroa mites through mitochondrial DNA and hypervariable nuclear loci. We applied an alternative RFLP technique and found that all the analyzed samples corresponded to Korean haplotypes. We analyzed the three STRs loci VD112, VD114 and VD016, previously described as highly variable and found new alleles in all of them, with an absolute allele size very different to those reported worldwide. We also detected genic and genotypic differentiation between samples from two nearby locations (P=0.08). We also tested a new RFLP method for mite haplotype discrimination with an intra-reaction positive control of digestion.

Characterization of <italic>Varroa destructor</italic> Mites in Cuba Using Mitochondrial and Nuclear Markers

Facultad de Ciencias, Universidad de la República

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Characterization of  Mites in Cuba Using Mitochondrial and Nuclear Markers

Varroa destructor has been present in Cuba since 1996, but without the use of acaricidal infestation rates remain at very low levels. The presence of Korean...

Allele size (bp)			Localities

Locus	sequenced ^*	this study	Mayabeque	La Habana
			N=12	N=12
VD112	142	158	0.04	0.04
		160	0.00	0.08
		168	0.88	0.58
		178	0.08	0.29
Ho			0.25	0.67
He			0.23	0.57
			N=11	N=12
VD114	217	246	0.09	0.08
VD114	217	250	0.91	0.92
Ho			0.00	0.00
He			0.17	0.15
			N=12	N=12
VD016	268	316	1	1
Ho			0	0
He			0	0

Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers

Article Category: Original Article

Online veröffentlicht: 24. Okt. 2020

Seitenbereich: 335 - 343

Eingereicht: 27. Mai 2020

Akzeptiert: 13. Juli 2020

DOI: https://doi.org/10.2478/jas-2020-0022

Schlüsselwörter
DNA mitochondrial, haplotype, microsatellites, STR

© 2020 Anais Rodríguez et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Microsatellites absolute sizes and frequencies of alleles found in Cuba. He and Ho are expected and observed heterocigocity, respectively

Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers

Article Category: Original Article

Online veröffentlicht: 24. Okt. 2020

Seitenbereich: 335 - 343

Eingereicht: 27. Mai 2020

Akzeptiert: 13. Juli 2020

DOI: https://doi.org/10.2478/jas-2020-0022

SchlüsselwörterDNA mitochondrial, haplotype, microsatellites, STR

© 2020 Anais Rodríguez et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Microsatellites absolute sizes and frequencies of alleles found in Cuba. He and Ho are expected and observed heterocigocity, respectively

Schlüsselwörter
DNA mitochondrial, haplotype, microsatellites, STR