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Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers

Anais Rodríguez

Anais Rodríguez

Centro de Investigaciones ApícolasLa Habana, Cuba
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Rodríguez, Anais
, 
Carlos A. Yadró

Carlos A. Yadró

Centro de Investigaciones ApícolasLa Habana, Cuba
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Yadró, Carlos A.
, 
Adolfo Pérez

Adolfo Pérez

Centro de Investigaciones ApícolasLa Habana, Cuba
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Pérez, Adolfo
, 
Ciro Invernizzi

Ciro Invernizzi

Facultad de Ciencias, Universidad de la RepúblicaMontevideo, Uruguay
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Invernizzi, Ciro
 et 
Ivanna Tomasco

Ivanna Tomasco

Facultad de Ciencias, Universidad de la RepúblicaMontevideo, Uruguay
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Tomasco, Ivanna
  
24 oct. 2020
Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers's Cover Image
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Catégorie d'article: Original Article
Publié en ligne: 24 oct. 2020
Pages: 335 - 343
Reçu: 27 mai 2020
Accepté: 13 juil. 2020
DOI: https://doi.org/10.2478/jas-2020-0022
Mots clés
© 2020 Anais Rodríguez et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Map of sampled locations. PR: Pinar del Rio, LH: La Habana, MY: Mayabeque, MT: Matanzas, LT: Las Tunas, HO: Holguín, SC: Santiago de Cuba, GT: Guantánamo. Black triangles represent localization on the sampled apiaries.
Map of sampled locations. PR: Pinar del Rio, LH: La Habana, MY: Mayabeque, MT: Matanzas, LT: Las Tunas, HO: Holguín, SC: Santiago de Cuba, GT: Guantánamo. Black triangles represent localization on the sampled apiaries.

Fig. 2

Restriction profiles of COI region of Varroa destructor mtDNA. Eight provinces of Cuba were sampled and displayed numbered from 1 to 8. 1: PR, 2: LH, 3: MY, 4: MT, 5: LT, 6: HO, 7: SC and 8:GT. The mtDNA was digested with endonucleases SacI and EcoNI. Lane L: 100-bp DNA ladder, Lane A: Undigested amplicons (amplified with COXF and COXRa primers) and Lane B: Undigested amplicons (amplified with VD-CO206F and VD-CO206R).
Restriction profiles of COI region of Varroa destructor mtDNA. Eight provinces of Cuba were sampled and displayed numbered from 1 to 8. 1: PR, 2: LH, 3: MY, 4: MT, 5: LT, 6: HO, 7: SC and 8:GT. The mtDNA was digested with endonucleases SacI and EcoNI. Lane L: 100-bp DNA ladder, Lane A: Undigested amplicons (amplified with COXF and COXRa primers) and Lane B: Undigested amplicons (amplified with VD-CO206F and VD-CO206R).

Microsatellites absolute sizes and frequencies of alleles found in Cuba_ He and Ho are expected and observed heterocigocity, respectively

Allele size (bp)Localities
Locussequenced *this studyMayabequeLa Habana
N=12N=12
VD1121421580.040.04
1600.000.08
1680.880.58
1780.080.29
Ho0.250.67
He0.230.57
N=11N=12
VD1142172460.090.08
2500.910.92
Ho0.000.00
He0.170.15
N=12N=12
VD01626831611
Ho00
He00
Langue:
Anglais
Périodicité:
2 fois par an
Sujets de la revue:
Sciences de la vie, Zoologie, Sciences de la vie, autres
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