<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Pratylenchus coffeae</italic>, belonging to the root-lesion nematode group, is a highly prevalent and destructive plant-parasitic nematode that is able to infest a wide range of host plants. Although this species' devastating impacts on coffee plantations across the world are widely known on other host plants, its association with Ming aralia has never been reported. Our study characterized two popu lations of <italic>P. coffeae</italic> (associated with Ming aralia and coffee) and compared them with other popu lations from previous studies in Vietnam and other countries in the world. The identification of <italic>P. coffeae</italic> in our study was confirmed by the comprehensive analysis encompassing morphological examination, morphometric data, and molecular characterizations of the <italic>COI</italic> mtDNA and D2D3 of 28S rRNA regions. The cluster and MDS analyses revealed that the two populations of <italic>P. coffeae</italic> from Vietnam are closely related to those from Japan and Indonesia. The D2-D3 sequences of 28S rRNA and <italic>COI</italic> mtDNA regions exhibited high similarity among these populations, indicating a stable genetic profile. Our research contributes to a better understanding of the distribution and genetic characterizations of <italic>P. coffeae</italic> by offering new morphological and molecular insights into the presence of this nematode in Vietnam. Additionally, this nematode species was found to be associated with host plant's symptoms such as chlorotic leaves, stunted growth and root lesion in both hosts. Given the economic significance of both Ming aralia and coffee crops in Vietnam, as well as the damaging potential of <italic>P. coffeae</italic>, this study emphasizes the need of proactive nematode management measures to control this destructive pest.</p>
</abstract>

Pratylenchus coffeae, belonging to the root-lesion nematode group, is a highly prevalent and destructive plant-parasitic nematode that is able to infest a wide range of host plants. Although this species' devastating impacts on coffee plantations across the world are widely known on other host plants, its association with Ming aralia has never been reported. Our study characterized two popu lations of P. coffeae (associated with Ming aralia and coffee) and compared them with other popu lations from previous studies in Vietnam and other countries in the world. The identification of P. coffeae in our study was confirmed by the comprehensive analysis encompassing morphological examination, morphometric data, and molecular characterizations of the COI mtDNA and D2D3 of 28S rRNA regions. The cluster and MDS analyses revealed that the two populations of P. coffeae from Vietnam are closely related to those from Japan and Indonesia. The D2-D3 sequences of 28S rRNA and COI mtDNA regions exhibited high similarity among these populations, indicating a stable genetic profile. Our research contributes to a better understanding of the distribution and genetic characterizations of P. coffeae by offering new morphological and molecular insights into the presence of this nematode in Vietnam. Additionally, this nematode species was found to be associated with host plant's symptoms such as chlorotic leaves, stunted growth and root lesion in both hosts. Given the economic significance of both Ming aralia and coffee crops in Vietnam, as well as the damaging potential of P. coffeae, this study emphasizes the need of proactive nematode management measures to control this destructive pest.

Morphological and molecular characterizations of <italic>Pratylenchus coffeae</italic> infecting Ming aralia and coffee in Vietnam

Department of plant pathology, Faculty of Agronomy

Vietnam National University of Agriculture

Graduate University of Science and Technology

Vietnam Academy of Sciences and Technology

Center for experiment and vocational training

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterizations of  infecting Ming aralia and coffee in Vietnam

Pratylenchus coffeae, belonging to the root-lesion nematode group, is a highly prevalent and destructive plant-parasitic nematode that is able to infest a wide...

Morphological and molecular characterizations of Pratylenchus coffeae infecting Ming aralia and coffee in Vietnam

Article Category: Research Article

Published Online: Apr 23, 2024

Page range: 76 - 84

Received: Jul 01, 2023

Accepted: Jan 19, 2023

DOI: https://doi.org/10.2478/helm-2024-0004

Keywords
Cluster analysis, COI, D2-D3, Lam Dong, MDS, systematics, taxonomy, variation, Vinh Phuc

© 2024 H. D. Nguyen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterizations of Pratylenchus coffeae infecting Ming aralia and coffee in Vietnam

Article Category: Research Article

Published Online: Apr 23, 2024

Page range: 76 - 84

Received: Jul 01, 2023

Accepted: Jan 19, 2023

DOI: https://doi.org/10.2478/helm-2024-0004

KeywordsCluster analysis, COI, D2-D3, Lam Dong, MDS, systematics, taxonomy, variation, Vinh Phuc

© 2024 H. D. Nguyen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Cluster analysis, COI, D2-D3, Lam Dong, MDS, systematics, taxonomy, variation, Vinh Phuc