Open Access

Comparative study of the effects of selenium nanoparticles and selenite on selenium content and nutrient quality in soybean sprouts

Shen Rao
Shen Rao
, 
Xian Xiao
Xian Xiao
, 
Yuan Wang
Yuan Wang
, 
Yuzhou Xiong
Yuzhou Xiong
, 
Hua Cheng
Hua Cheng
, 
Li Li
Li Li
 and 
Shuiyuan Cheng
Shuiyuan Cheng
   | Dec 31, 2022
Folia Horticulturae's Cover Image
About this article
Previous Article
Next Article
Cite
Article Category: Original Article
Published Online: Dec 31, 2022
Page range: 223 - 234
Received: Jan 21, 2022
Accepted: Oct 25, 2022
DOI: https://doi.org/10.2478/fhort-2022-0017
Keywords
© 2022 Shen Rao et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Biomass statistics of soybean sprouts. Different letters above the columns indicate significant differences at p < 0.05.
Biomass statistics of soybean sprouts. Different letters above the columns indicate significant differences at p < 0.05.

Figure 2

Total Se concentrations in the shoots (A) and roots (B) of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05.
Total Se concentrations in the shoots (A) and roots (B) of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05.

Figure 3

Chlorophyll (A) and carotenoid (B) contents of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05. SeNPs, selenium nanoparticles.
Chlorophyll (A) and carotenoid (B) contents of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05. SeNPs, selenium nanoparticles.

Figure 4

Soluble sugar (A) and protein (B) contents of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05. SeNPs, selenium nanoparticles.
Soluble sugar (A) and protein (B) contents of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05. SeNPs, selenium nanoparticles.

Figure 5

Vitamin C (A) and total isoflavone (B) contents of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05. SeNPs, selenium nanoparticles.
Vitamin C (A) and total isoflavone (B) contents of soybean sprouts treated with SeNPs and selenite. Different letters above the columns indicate significant differences at p < 0.05. SeNPs, selenium nanoparticles.

Figure 6

Changes in the MDA and antioxidant system of soybean sprouts treated with SeNPs and selenite. (A) MDA content; (B) GSH content; (C) CAT activity; (D) POD activity; (E) SOD activity and (F) APX activity. Different letters above the columns indicate significant differences at p < 0.05. APX, ascorbate peroxidase; CAT, catalase; GSH, glutathione; MDA, malonaldehyde; POD, peroxidase; SeNPs, selenium nanoparticles; SOD, superoxidase dismutase.
Changes in the MDA and antioxidant system of soybean sprouts treated with SeNPs and selenite. (A) MDA content; (B) GSH content; (C) CAT activity; (D) POD activity; (E) SOD activity and (F) APX activity. Different letters above the columns indicate significant differences at p < 0.05. APX, ascorbate peroxidase; CAT, catalase; GSH, glutathione; MDA, malonaldehyde; POD, peroxidase; SeNPs, selenium nanoparticles; SOD, superoxidase dismutase.

Figure S1

The spectrogram of the Se speciations by LC-AFS. LC-AFS, liquid chromatography-atomic fluorescence spectrometry; Se4+, selenite; Se6+, selenate; SeCys2, selenocystine; SeMeCys, methyl selenocysteine; SeMet, selenomethionine.
The spectrogram of the Se speciations by LC-AFS. LC-AFS, liquid chromatography-atomic fluorescence spectrometry; Se4+, selenite; Se6+, selenate; SeCys2, selenocystine; SeMeCys, methyl selenocysteine; SeMet, selenomethionine.

Selenium speciation in soybean shoots and roots.

Tissue Treatment Selenium speciation (μg · g−1 DW)
SeCys2 MeSeCys SeMet Se4+ Se6+
Shoot 0 nd nd 0.193 ± 0.021 d 0.035 ± 0.008 c nd
N20 0.188 ± 0.022 b 0.185 ± 0.048 c 2.182 ± 0.258 c 0.071 ± 0.023 c 0.453 ± 0.001 b
N100 1.192 ± 0.199 a 0.953 ± 0.097 a 3.789 ± 0.611 b 0.147 ± 0.025 b 0.792 ± 0.092 a
S20 0.093 ± 0.019 b 0.087 ± 0.008 d 1.857 ± 0.189 c 0.048 ± 0.005 c nd
S100 0.969 ± 0.041 a 0.696 ± 0.012 b 4.796 ± 0.065 a 0.248 ± 0.006 a nd
Root 0 nd nd 0.127 ± 0.015 d 0.034 ± 0.008 e nd
N20 2.245 ± 0.214 d 2.292 ± 0.286 c 3.897 ± 0.240 c 0.888 ± 0.051 d 2.444 ± 0.057 b
N100 6.318 ± 0.377 b 7.224 ± 0.336 b 5.734 ± 0.250 b 1.759 ± 0.066 b 6.870 ± 0.356 a
S20 3.613 ± 0.084 c 2.188 ± 0.255 c 3.601 ± 0.178 c 1.046 ± 0.015 c nd
S100 11.135 ± 0.716 a 8.698 ± 0.235 a 14.213 ± 0.059 a 1.970 ± 0.021 a nd
eISSN:
2083-5965
Language:
English
Publication timeframe:
2 times per year
Journal Subjects:
Life Sciences, Plant Science, Zoology, Ecology, other
Journal RSS Feed