<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Natural kefir grains are rich in beneficial bacteria, and analysis of their microbial diversity is a necessary condition for developing and applying kefir grains. In this study, six commercially available natural kefir grains were used as raw materials to explore their microbial diversity by metagenomics. The results showed that there were 14794 genes in 6 kinds of natural kefir grains, and the number of unique genes of X1, X2, X3, X4, X5, X6 were 111, 11, 0, 1899, 552, 1, respectively. From the relative abundance table of boundary, phylum, class, order, family, genus and species, the microbial diversity at each level was analyzed.The two dominant genera at the genus level are <italic>Lactobacillus</italic> and <italic>Lactococcus</italic>, and the dominant species at the species level are <italic>Lactococcus lactis</italic> and <italic>Lactococcus kefiranofaciens</italic>, <italic>Lactococcus crispatus</italic>, and <italic>Lactococcus helveticus</italic>, etc. Species distribution and species diversity of each sample were analyzed by species heat map, principal component analysis and non-metric multidimensional calibration methods. The results showed that the microbial diversity of natural kefir grains from 6 different sources were different. The research can provide reference for the development and application of natural kefir grains in the field of dairy products.</p>
</abstract>

Natural kefir grains are rich in beneficial bacteria, and analysis of their microbial diversity is a necessary condition for developing and applying kefir grains. In this study, six commercially available natural kefir grains were used as raw materials to explore their microbial diversity by metagenomics. The results showed that there were 14794 genes in 6 kinds of natural kefir grains, and the number of unique genes of X1, X2, X3, X4, X5, X6 were 111, 11, 0, 1899, 552, 1, respectively. From the relative abundance table of boundary, phylum, class, order, family, genus and species, the microbial diversity at each level was analyzed.The two dominant genera at the genus level are Lactobacillus and Lactococcus, and the dominant species at the species level are Lactococcus lactis and Lactococcus kefiranofaciens, Lactococcus crispatus, and Lactococcus helveticus, etc. Species distribution and species diversity of each sample were analyzed by species heat map, principal component analysis and non-metric multidimensional calibration methods. The results showed that the microbial diversity of natural kefir grains from 6 different sources were different. The research can provide reference for the development and application of natural kefir grains in the field of dairy products.

Microbial Diversity of Six Commercially Available Kefir Grains

Shaanxi University of Science and Technology

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Microbial Diversity of Six Commercially Available Kefir Grains"}

Natural kefir grains are rich in beneficial bacteria, and analysis of their microbial diversity is a necessary condition for developing and applying kefir...

Microbial Diversity of Six Commercially Available Kefir Grains

Published Online: Dec 30, 2022

Page range: 287 - 292

Received: Sep 08, 2022

Accepted: Nov 07, 2022

DOI: https://doi.org/10.2478/aucft-2022-0023

Keywords
kefir grains, metagenomics, microbial diversity, species annotation

© 2022 Xinxin Dong et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Microbial Diversity of Six Commercially Available Kefir Grains

Published Online: Dec 30, 2022

Page range: 287 - 292

Received: Sep 08, 2022

Accepted: Nov 07, 2022

DOI: https://doi.org/10.2478/aucft-2022-0023

Keywordskefir grains, metagenomics, microbial diversity, species annotation

© 2022 Xinxin Dong et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
kefir grains, metagenomics, microbial diversity, species annotation