The Effect of Using DNA Obtained from Blood of Cattle with Genetic Chimerism on Illumina’s Beadchip Assay Performance
Published Online: Apr 25, 2014
Page range: 279 - 286
Received: Oct 22, 2013
Accepted: Jan 20, 2014
DOI: https://doi.org/10.2478/aoas-2014-0011
Keywords
© by Artur Gurgul
This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Blood cell chimerism is a common phenomenon occurring in cattle coming from double or multiple parturitions and can be observed as two DNA profiles present in blood of each of twin born animals. In the era of genomics, a large number of animals is being genotyped with high throughput genotyping methods, which are giving limited insight into the performance of single markers and rather only statistical description of the results is available for a common user. This hampers the detailed analysis of the results obtained and direct identification of the causes of poorer performance of some samples. In this study we describe the influence of analysis of DNA obtained from blood samples of cattle with genetic chimerism on basic parameters of Infinium technology-based Illumina’s genotyping arrays. The results obtained may help to identify such samples, especially when no precise information about the animals’ origin is available