<abstract xmlns="http://www.w3.org/1999/xhtml">

Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper, direct chiral separation of lactic acid by high performance liquid chromatography is presented. Five chiral stationary phases based on macrocyclic antibiotics were used for enantioseparation and chromatographic parameters, such as retention factors, resolution and selectivity factors, were determined under different column temperatures ranging from 5 to 45 °C. Optical isomers of lactic acid were efficiently separated using chiral stationary phases based on teicoplanin (<italic>R</italic>S = 1.9
) and ristocetin (<italic>R</italic>S = 1.7
) in reversed-phase separation mode at the column temperature of 25 °C.
</abstract>

Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper, direct chiral separation of lactic acid by high performance liquid chromatography is presented. Five chiral stationary phases based on macrocyclic antibiotics were used for enantioseparation and chromatographic parameters, such as retention factors, resolution and selectivity factors, were determined under different column temperatures ranging from 5 to 45 °C. Optical isomers of lactic acid were efficiently separated using chiral stationary phases based on teicoplanin (RS = 1.9
) and ristocetin (RS = 1.7
) in reversed-phase separation mode at the column temperature of 25 °C.

Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

Slovak University of Technology in Bratislava

Faculty of Chemical and Food Technology, Institute of Analytical Chemistry, Radlinského 9

Acta Chimica Slovaca

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper,...

Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

Published Online: Sep 10, 2021

Page range: 60 - 65

DOI: https://doi.org/10.2478/acs-2021-0008

Keywords
chiral separation, high performance liquid chromatography, lactic acid, macrocyclic antibiotics, temperature

© 2021 Laura Jánovová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

Published Online: Sep 10, 2021

Page range: 60 - 65

DOI: https://doi.org/10.2478/acs-2021-0008

Keywordschiral separation, high performance liquid chromatography, lactic acid, macrocyclic antibiotics, temperature

© 2021 Laura Jánovová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
chiral separation, high performance liquid chromatography, lactic acid, macrocyclic antibiotics, temperature