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Expression of genes involved in the inflammatory response in human granulosa cells in short-term in vitro culture

Sandra Kałużna

Sandra Kałużna

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Kałużna, Sandra
, 
Rut Bryl

Rut Bryl

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Bryl, Rut
, 
Błażej Chermuła

Błażej Chermuła

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Chermuła, Błażej
, 
Rafał Sibiak

Rafał Sibiak

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Sibiak, Rafał
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Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Stefańska, Katarzyna
, 
Wojciech Pieńkowski

Wojciech Pieńkowski

Division of Perinatology and Women’s Diseases, Poznań University of Medical SciencesPoznań, Poland
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Pieńkowski, Wojciech
, 
Wiesława Kranc

Wiesława Kranc

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Kranc, Wiesława
, 
Michal Jeseta

Michal Jeseta

Department of Obstetrics and Gynecology, University Hospital and Masaryk UniversityBrno, Czech Republic
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Jeseta, Michal
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Pavel Ventruba

Pavel Ventruba

Department of Obstetrics and Gynecology, University Hospital and Masaryk UniversityBrno, Czech Republic
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Ventruba, Pavel
, 
Jana Zakova

Jana Zakova

Department of Obstetrics and Gynecology, University Hospital and Masaryk UniversityBrno, Czech Republic
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Zakova, Jana
, 
Igor Crha

Igor Crha

Department of Obstetrics and Gynecology, University Hospital and Masaryk UniversityBrno, Czech Republic
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Crha, Igor
, 
Leszek Pawelczyk

Leszek Pawelczyk

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Pawelczyk, Leszek
 and 
Robert Z. Spaczyński

Robert Z. Spaczyński

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Spaczyński, Robert Z.
  
Dec 31, 2020
Expression of genes involved in the inflammatory response in human granulosa cells in short-term in vitro culture's Cover Image
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Published Online: Dec 31, 2020
Page range: 190 - 195
Received: Nov 18, 2020
Accepted: Dec 21, 2020
DOI: https://doi.org/10.2478/acb-2020-0025
Keywords
© 2020 Sandra Kałużna, Rut Bryl, Błażej Chermuła, Rafał Sibiak, Katarzyna Stefańska, Wojciech Pieńkowski, Wiesława Kranc, Michal Jeseta, Pavel Ventruba, Jana Zakova, Igor Crha, Leszek Pawelczyk, Robert Z. Spaczyński, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

The change in the expression of IL1B, IL6 and TNFa genes in 48h and 72h in short-term in vitro culture of human ovarian granulosa cells, as compared to the expression in 24 hours of culture
The change in the expression of IL1B, IL6 and TNFa genes in 48h and 72h in short-term in vitro culture of human ovarian granulosa cells, as compared to the expression in 24 hours of culture

Oligonucleotide sequences of primers used for RT-qPCR analysis

GENEPRIMER SEQUENCE (5’–3’)PRODUCT SIZE (BP)
IL1βF GGGCCTCAAGGAAAAGAATCR TTCTGCTTGAGAGGTGCTGA205
IL6F ACAACCTGAACCTTCCAAAGAR ACAACCTGAACCTTCCAAAGA179
TNFαF AGCCCATGTTGTAGCAAACCR AGCCCATGTTGTAGCAAACC157
ACTBF AAAGACCTGTACGCCAACACR CTCAGGAGGAGCAATGATCTTG132
GAPDHF TCAGCCGCATCTTCTTTTGCR ACGACCAAATCCGTTGACTC90
HPRTF TGGCGTCGTGATTAGTGATGR ACATCTCGAGCAAGACGTTC141
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English
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Journal Subjects:
Life Sciences, Molecular Biology, Biochemistry
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