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The influence of osteogenic differentiation on the stem-like properties of adipose derived stem cells – an RT-qPCR study

Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Bryl, Rut
, 
Claudia Dompe

Claudia Dompe

School of Medicine, Medical Sciences and Nutrition, University of AberdeenAberdeen, UK
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Dompe, Claudia
, 
Maurycy Jankowski

Maurycy Jankowski

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Jankowski, Maurycy
, 
Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Stefańska, Katarzyna
, 
Afsaneh Golkar Narenji

Afsaneh Golkar Narenji

Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, NC, USA
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Narenji, Afsaneh Golkar
, 
Jakub Kulus

Jakub Kulus

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToru, Poland
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Kulus, Jakub
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Magdalena Kulus

Magdalena Kulus

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Kulus, Magdalena
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Maria Wieczorkiewicz

Maria Wieczorkiewicz

Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in ToruńToruń, Poland
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Wieczorkiewicz, Maria
, 
Grzegorz Wąsiatycz

Grzegorz Wąsiatycz

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Wąsiatycz, Grzegorz
, 
Jędrzej M. Jaśkowski

Jędrzej M. Jaśkowski

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToru, Poland
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Jaśkowski, Jędrzej M.
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Mariusz Kaczmarek

Mariusz Kaczmarek

Department of Cancer Immunology, Poznan University of Medical SciencesPoznań, Poland
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Kaczmarek, Mariusz
, 
James N. Petitte

James N. Petitte

Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, NC, USA
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Petitte, James N.
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Paul Mozdziak

Paul Mozdziak

  • Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, NC, USA
  • Physiology Graduate Program, North Carolina State UniversityRaleigh, NC, USA
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Mozdziak, Paul
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Paweł Antosik

Paweł Antosik

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Antosik, Paweł
 and 
Dorota Bukowska

Dorota Bukowska

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToru, Poland
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Bukowska, Dorota
  
Dec 31, 2020
The influence of osteogenic differentiation on the stem-like properties of adipose derived stem cells – an RT-qPCR study's Cover Image
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Published Online: Dec 31, 2020
Page range: 158 - 163
Received: Oct 21, 2020
Accepted: Dec 03, 2020
DOI: https://doi.org/10.2478/acb-2020-0020
Keywords
© 2020 Rut Bryl, Claudia Dompe, Maurycy Jankowski, Katarzyna Stefańska, Afsaneh Golkar Narenji, Jakub Kulus, Magdalena Kulus, Maria Wieczorkiewicz, Grzegorz Wąsiatycz, Jędrzej M. Jaśkowski, Mariusz Kaczmarek, James N. Petitte, Paul Mozdziak, Paweł Antosik, Dorota Bukowska, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

The results of the RT-qPCR analysis of the change in expression of ASC specific markers before and after osteogenic differentiation. All data was presented as a log2 of fold change
The results of the RT-qPCR analysis of the change in expression of ASC specific markers before and after osteogenic differentiation. All data was presented as a log2 of fold change

The sequences of primers used in the analysis

GENE NAMEFORWARD PRIMERREVERSE PRIMER
CD105CTCAGGTCCCCAATGCTACCGGTTGAAGGCCAGGTAGAGT
CD73CCCATTGACGAACGGAACAATATACCACGTGAATTCCGCC
CD14CACTAGAGCCCTGCGAAGTACGACGGCAATCATACACTGG
CD34ATGAGACCTCCAGCTGTGAGAGGTCAGACTGGTGCTTTCT
CD90CGAGAATGCTACCACCTTGCAGCCGGAGTTCACATGTGTA
CD45ACCTAGGCAAACATGTGAGGACTTCCAGATCAAAATTTCCACGA
HPRTCCATCACATCGTAGCCCTCACTTTTATATCGCCCGTTGAC
ACTBCCCTTGCCGCTCCGCCTTCGCAGCAATATCGGTCATCCAT
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Biochemistry
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