Cmpd | IC50 (μM) IC50 is the concentration of the triazoles and triazolium salts inducing 50% cell growth inhibition after 72 h incubation. The results are shown as mean values of at least three experiments (± SD). |
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1a | > 100 |
1b | > 100 |
1f | > 100 |
2a | 91.6 ± 3.9 |
2b | 57.0 ± 12.9 |
2c | > 100 |
2d | > 100 |
2e | 55.4 + 9.4 |
2f | – Triazolium salt precipitated promptly after the addition to the growth medium and thus the cytotoxicity could not be measured accurately. |
2g | – The range of concentrations 10 – 1000 μM reduced survival from about 60 to 40%, and therefore the exact IC50 was difficult to determine. |
2h | 88.9 + 7.5 |
2i | 54.4 + 14.7 |
2j | 54.9 + 3.5 |
2k | > 100 |
2l | – The range of concentrations 10 – 1000 μM reduced survival from about 60 to 40%, and therefore the exact IC50 was difficult to determine. |
Conc. (μM) | 24 h | 48 h | 72 h | |||||||||
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G1 | S | G2/M | subG1 | G1 | S | G2/M | subG1 | G1 | S | G2/M | subG1 | |
0 | 54 | 31 | 15 | 2 | 59 | 26 | 15 | 2 | 73 | 13 | 14 | 2 |
29.7 | 61 | 24 | 15 | 2 | 61 | 26 | 13 | 5 | 65 | 17 | 18 | 6 |
110 | 78 | 9 | 13 | 3 | 78 | 9 | 13 | 15 | 79 | 5 | 16 | 35 |
Cell line | IC50 (μM) IC50 is the concentration of 2b that induces 50% cell growth inhibition after 72 h incubation. | T.I. T.I. refers to therapeutic index, calculated from the ratio of cytotoxicity (IC50) on normal fibroblasts and cytotoxicity (IC50) on tumour cells. |
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HeLa | 57.0 ± 12.9 | 4.01 |
HEp-2 | 87.0 ± 28.5 | 2.63 |
7T | 111.7 ± 7.6 | 2.05 |
H460 | 29.7 + 4.5 | 7.69 |
HCT-116 | 51.9 ± 7.2 | 4.40 |
Fibroblasts | 228.5 ± 5.6 | – |