1. bookVolume 27 (2014): Issue 3 (September 2014)
Journal Details
License
Format
Journal
eISSN
2300-6676
ISSN
2084-980X
First Published
30 May 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

Published Online: 03 Mar 2015
Volume & Issue: Volume 27 (2014) - Issue 3 (September 2014)
Page range: 142 - 144
Received: 20 Aug 2014
Accepted: 26 Aug 2014
Journal Details
License
Format
Journal
eISSN
2300-6676
ISSN
2084-980X
First Published
30 May 2014
Publication timeframe
4 times per year
Languages
English
ABSTRACT

In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively.

Keywords

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