Tom 66 (2022): Zeszyt 4 (December 2022)

The health of the gastrointestinal system of pigs is still a topical issue. When focusing on the youngest categories of pigs, we routinely evaluate it on the basis of appetite assessment and physical examination of faeces. A piglet’s gut health is also related to the development and changes of pH in the digestive system. Because there is little scientific work in this area, the aim of this study was to evaluate the physiological range of faecal pH in healthy suckling piglets from birth to weaning. Faecal pH measurements were performed in thirty-five suckling piglets at the time of the piglets’ birth, on the 7th, 14th, 21st, and 28th day of their life. We found the mean pH values to be 6.38 ± 0.46 within 24 hours after birth; 7.14 ± 0.23 on the 7th day after birth; 7.23 ± 0.23 on the 14th day after birth; 7.42 ± 0.60 on the 21st day after birth; and 7.72 ± 0.61 on the 28th day after birth. Statistical analysis showed significant differences between the pH of the faeces of new-born piglets and the samples taken in the following weeks of the experiment (P < 0.05; P < 0.01; P < 0.001). Measuring faecal pH can be a simple, quick and inexpensive method used to determine the health status of piglets’ intestines.

This case report describes a practical approach for diagnosing and treating a 17-month-old Syrian hamster with hyperadrenocorticism based on: history, systemic signs, dermatological lesions, and therapeutic trial. The patient was monitored for 16 weeks while he was treated with trilostane and achieved hair regrowth and the resolution of systemic and demeanour signs.

The genus Malassezia is a medically important genus of yeasts that can colonize the skin of humans and other warm-blooded animals. The genus currently comprises 18 species of which four new species were identified recently. The most widely known species, M. pachydermatis, occurs in animals but was detected also in humans, namely at life endangering septicaemias and in prematurely born children. Proliferation of Malassezia occurs most frequently as a result of disturbances in the normal homeostasis of host immunity on the one hand and virulence of these yeasts on the other hand. The successful management of the disease depends on the therapeutic control of overgrowth of the yeasts and any concurrent bacterial infection by local or systemic anti microbial treatment, as well as, on identification and potential correction of the predisposing factors.

About 150 million families around the world are engaged in milk production. However, inflammation of the mammary gland (mastitis) remains a major problem in dairy ruminants that affects the quality of milk worldwide. The aim of this study was the examination of udder health with detection of contagious and environmental pathogens causing mastitis in 960 and 940 dairy cows and ewes, respectively. The presence of selected virulence factors such as: the formation of haemolysis, gelatinase, biofilm, hydrolyse DNA, and resistance to antibiotics with detection of methicillin resistance gene (mecA), were determined in selected virulence factors associated with isolated staphylococci. These isolated staphylococci with selected virulence factors can have untoward effects on the severity of mastitis. The results of our study indicated that, in addition to the major udder pathogens (S. aureus, S. uberis, and S. agalactiae) causing mastitis, non-aureus staphylococci (NAS), is a major risk to dairy cows and ewes. NAS, such as S. chromogenes, S. warneri, and S. xylosus isolated from infected animals with clinical and chronic mastitis, had the highest representation of virulence factors in comparison to less virulent strains. In addition, the isolates of S. aureus and NAS demonstrated 77.0 % and 44.2 % resistance to one or more antimicrobial classes from mastitic milk samples obtained from dairy cows and ewes, respectively. Due to the high resistance to β-lactamantibiotics in two isolates of S. aureus and two species of NAS isolated from cows’ mastitic milk samples, the presence of a methicillin-resistant gene mecA poses serious complications for the treatment and a serious health risk to milk consumers.

This study is focused on determining the cryotherapeutic effects in the treatment and welfare of dogs. We characterized the basic principles of cryotherapy as well as summarized and statistically processed the current state of the application of this form of therapy in a veterinary practice. Recent scientific studies have shown that cryotherapy is mainly effective in treating skin diseases and problems with the musculoskeletal system including minor injuries caused by excessive muscle strain. It can also be used in combination with anti-inflammatory drugs to treat the respiratory system. Moreover, cryotherapy can be an effective form of treatment for ageing. Our study involved a survey evaluation given to veterinarians in Slovakia, Czechia, and Hungary with a key question regarding their experiences with cryotherapy and cryosurgery in dog therapy. Statistical results demonstrated that Slovak veterinarians do not utilize cryo-methods satisfactorily. A summarization of the reasons could start a change in this unfavourable aspect in Slovak veterinary medicine and contribute to better promotion of cryotherapy application in the therapy of animals.

The aim of this study was to evaluate the relationship between nutrition, the concentration of milk urea nitrogen (MUN) and the urinary nitrogen excretion in farm conditions and to use the MUN concentration as a tool to control protein nutrition and environmental load in dairy farming. Urinary N excretion was evaluated by an empirical model according to the intake and metabolic transformation of N to milk protein in selected farms was on average 208.8 ± 34.8 g with a range of values from 127.7 to 277.8 g N.day⁻¹. The evaluated proportion of excreted N in relation to crude protein (CP) intake in the total mix ration (TMR) was statistically significant (R² = 0.504; P < 0.0001). Urinary N excretion, evaluated according to the analysis of the MUN content, using selected regression equations, was on average 211.8 ± 24.3 g.day⁻¹ with an individual variation of 157.2‒274.7 g.day⁻¹ with a significantly higher positive correlation to the received CP in the TMR (R² = 0.693; P < 0.0001). The evaluated effect of CP concentration in the TMR on urine N excretion confirmed the higher nitrogen excretion in the urine by 25.6 g per day with an increased CP in the TMR by 1 %. The proportion of urea nitrogen in the total N excreted in the urine was on average 80.5 %. The validation of the models for the prediction of nitrogen excretion, according to the MUN for the practical application on farms, was determined the best equation by Kaufmann a St-Pierre, which used available data from routine analysis of milk composition by the Breeding service of Slovakia. The MUN analysis offered a simple and non-invasive approach to the evaluation of the urinary N excretion, as well as, the efficiency of N utilization from feed to milk.

This study was conducted to investigate the prevalence of subclinical mastitis and its contagious and environmental bacterial causes in dairy cows in Jos Metro polis of Plateau State. A total of 208 milk samples were collected aseptically from 52 lactating cows and subjected to the California Mastitis Tests (CMT). Milk samples were transferred into peptone water broth for enrichment, followed with bacteriological assays and biochemical identification of bacteria. Using R Commander version 3.6.2, data such as: breed, age, parity, lactation, and management system of cows were collected, and analysed to determine their relationship with bovine subclinical mastitis. Out of 208 quarter milk samples collected from 52 cows, 35.09 % showed subclinical mastitis. While the Cross-breed cows had 15 % subclinical mastitis recorded, the White Fulani had 78.13 %. Cows of the age 3 to 5 years had the highest prevalence of mastitis, while cows with 4 to 6 calves showed the highest prevalence in the parity category. The early lactation stage of these cows showed the highest prevalence. The environmental bacteria isolated where E. coli, K. pneumoniae, K. oxytoca, C. freundii, K. aerogenes, and Proteus sp. with E. coli having the highest frequency of 40.86 %, followed by K. Pneumoniae with 17.79 %. The contagious bacteria isolated were S. aureus, S. agalactiae, and Corynebacterium sp. with S. aureus occurring most with 69.71 %. This study concluded that the prevalence of subclinical mastitis is high in cows in the study area. It was also discovered that the breed of cow, age of cow, parity number, stage of lactation and husbandry systems have an impact on subclinical mastitis. S. aureus with the highest frequency, indicated that contagious microbes have more effect on subclinical mastitis than environmental microbes.

Cadmium (Cd) is a common environmental pollutant present in soil and associated with many modern industrial processes. Cadmium may adversely influence the health of experimental animals and humans and exert significant effects on the reproductive tract morphology and physiology. During embryonic development, cadmium suppresses the normal growth and development of the ovaries, and in adults it disrupts the morphology and function of the ovaries and uterus. The exposure to cadmium has adverse effects on the oocyte meiotic maturation affecting the structure of ovarian tissue. The distribution of follicles and corpus luteum in the ovarian tissues has been shown to be disrupted, affecting the normal growth and development of the follicles. In the ovarian cortex, the number of follicles at different stages of maturation decreased, and the number of atretic follicles increased. In the medulla, oedema and ovarian haemorrhage and necrosis appears at higher doses. Granulosa cells exposed to cadmium exhibited morphological alterations. Oocyte development was inhibited and the amount of oocyte apoptosis was higher. Cadmium exposure also caused changes in the structure of the ovarian blood vessels with reduction in the vascular area. Cadmium effects included increased uterine weight, hyperplasia and hypertrophy of the endometrial lining. Exposure to cadmium had specific effects on gonadal steroidogenesis by suppressing steroid biosynthesis of the ovarian granulosa cells and luteal cells. Progesterone, follicle stimulating hormone, and luteinizing hormone decreased significantly after CdCl₂ administration. Cadmium can suppress the female’s ovulation process and cause temporary infertility.

The occurrence of extensive non-healing and chronic skin defects with loss of tissue substance in companion animals are a frequently solved problem. The management of the therapy of large-area defects and absent tissues is a challenge for setting up successful therapeutic management and achieving wound closure, satisfactory cosmetic effect, and restoration of the functionality of the damaged area. In veterinary medicine, we often encounter the failure of wound therapy methods commonly used in closing defects, as a result of which the defect closure time is prolonged. The longer the time required to close the defect, the greater the risk of microbial infection and complications associated with healing in the case of extensive damage to the surrounding soft tissues. Direct influence of the individual phases of healing with supportive alternative therapy appears to be a very suitable solution for the treatment of chronic wounds. To overcome the shortcomings related to partially efficient conventional wound dressings, efforts are oriented toward developing new and effective platforms for wound healing applications. Five patients referred to the Small Animal Clinic of the University Veterinary Hospital were included in this clinical study.

Patients were referred to the clinic with extensive long-term non-healing wounds, necrosis and secernation, or ongoing infection, with loss of tissue substance, which showed signs of chronicity. After assessing the patient’s state of health and subsequent cleaning of the wound bed from contaminants and damaged tissues, collagen sponge Suprasorb^® C was applied to the surface of the cleaned wound bed. The average time for the complete closure of the defect was 24.6 days since the introduction of the primary treatment. In all patients, we observed the complete closure of the defect, restoration of functionality of the damaged tissues, and achievement of a cosmetic effect without complications in the recovery process, which points to the excellent effectiveness of the collagen covering in the wound healing process.

Inherited eye diseases have been the subject of genetic research for many years. This paper focuses on the optimisation of the DNA test based on the polymerase chain reaction (PCR) for the detection of Collie Eye Anomaly (CEA) in dogs. A small family of four longhaired Collies (parents and their daughters) with a confirmed positive clinical ophthalmologic examination of CEA served as the source of affected animals. Both PCR reaction conditions examined were suitable for detecting canine NHEJ1 gene mutation associated with CEA. One carrier was found in a small group of eleven randomly selected control healthy dogs. The PCR test confirmed the previous CEA-positive ophthalmological examination in Collies. The results indicated that all four family members of the examined longhaired Collies had a homozygous intronic deletion of 7799 bases in the canine NHEJ1 gene. The affected female Collies may potentially transmit this CEA-associated mutation to their puppies.