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MAGI group activity - Research, diagnosis and treatment of genetic and rare diseases

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EBTNA Utility Gene Test on Ophthalmology

Informacje o czasopiśmie
Format
Czasopismo
eISSN
2564-615X
Pierwsze wydanie
30 Jan 2017
Częstotliwość wydawania
4 razy w roku
Języki
Angielski

Wyszukiwanie

Tom 1 (2017): Zeszyt 2 (May 2017)

Informacje o czasopiśmie
Format
Czasopismo
eISSN
2564-615X
Pierwsze wydanie
30 Jan 2017
Częstotliwość wydawania
4 razy w roku
Języki
Angielski

Wyszukiwanie

18 Artykułów
Otwarty dostęp

Ricinus communis L. (Castor bean), a potential multi-purpose environmental crop for improved and integrated phytoremediation

Data publikacji: 09 May 2017
Zakres stron: 101 - 116

Abstrakt

Abstract

Phytoremediation is a plant based environmental cleanup technology to contain (rendering less toxic), sequester and degrade contaminated susbtrates. As can be seen from data metrics, it is gaining cosiderable importance globally. Phytoremediation approach is being applied for cleanup of inorganic (potentially toxic metals), organic (persistent, emergent, poly-acromatic hydrocarbons and crude oil etc.) and co-contaminated (mixture of inorganic and organic) and/or polluted sites globally. Recently new approaches of utilizing abundantly available natural organic amendments have yielded significant results. Ricinus communis L. (Castor bean) is an important multipurpose crop viz., Agricultural, Energy, Environmental and Industrial crop. The current status of knowledge is abundant but scattered which need to be exploited for sustainable development. This review collates and evaluates all the scattered information and provides a critical view on the possible options for exploiting its potential as follows: 1. Origin and distribution, 2. Lead toxicity bioassays, 3. Progress in arbuscular mycorrhizal fungi-assisted phytoremediation, 4. Promising bioenergy crop that can be linked to pytoremediation, 5. A renewable source for many bioproducts with rich chemical diversity, 6. It is a good biomonitor and bioindicator of atmospheric pollution in urban areas, 7. Enhanced chelate aided remediation, 8. Its rhizospheric processes accelerate natural attenuation, 9. It is suitable for remediation of crude oil contaminated soil, 10. It is an ideal candidate for aided phytostabilization, 11. Castor bean is a wizard for phytoremediation and 12. Its use in combined phytoextraction and ecocatalysis. Further, the knowledge gaps and scope for future research on sustainable co-generation of value chain and value addition biobased products for sustainable circular economy and environmental security are described in this paper.

Otwarty dostęp

Influence of OASL gene polymorphisms on host response to interferon therapy in chronic hepatitis C virus patients

Data publikacji: 09 May 2017
Zakres stron: 117 - 125

Abstrakt

Abstract

Hepatitis C virus (HCV) infection becomes a major public health problem and leading cause of chronic liver disease and liver failure. Pegylated interferon-alfa and ribavirin are currently the standard treatment for chronic hepatitis C (CHC). It is indicated that genes that trace the interferon signaling could be associated with the host response to the therapy. In order to investigate the influence of these genes on host related response, we have analyzed seven single nucleotide polymorphisms (rs59248852, rs74390571, rs12811390, rs1169279, rs3213545, rs1083129 and rs2859398) in 2-5-Oligoadenylate- Synthetase-Like (OASL) gene in CHC cases from Republic of Macedonia. A simple and easy to use SNaPshot method was developed. A cohort of 100 HCV RNA positive patients - non responders and 109 patients with achieved virological response after the standard treatment were included in this study. We have found significant association in five of the seven studied SNP` s: rs59248852 [p = 6.5x10-31, OR=55.7 (20.0-155.3)]; rs12811390 [p = 2.2x10-11, OR=4.3 (2.3-6.7)]; rs2859398 [p=1.34x10-9, OR=3.4 (2.2-5.0)]; rs74390571 [p=4.3x10-7, OR=2.9 (1.9-4.4)], and rs1083129 [p=0.0139, OR=2.0 (1.1-3.5)]. The results from this study can be used as a predictive factor of future patient’s selection for the standard therapy, having an important cost benefit for the health insurance system.

Otwarty dostęp

Epilobium angustifolium L.: A medicinal plant with therapeutic properties

Data publikacji: 09 May 2017
Zakres stron: 126 - 131

Abstrakt

Abstract

Epilobium angustifolium L. is a medicinal plant belonging to the Onagraceae family, which includes more than 200 different species from all over the world. Traditional medicinal applications include treatment of prostate, gastrointestinal, menstrual disorders and recently it has been used for its analgesic and anti-inflammatory activity. In this investigation E. angustifolium was collected in Ternopil region of Ukraine. The obtained data demonstrated that E. angustifolium herb extract, rich in polyphenolic compounds such as flavonoids and tannins, display high antioxidant properties. In addition the potential anticancer activity has been investigated in vitro on human hepatocellular carcinoma cells (HepG2). Furthermore the cytotoxic and genotoxic effects of E. angustifolium have been investigated respectively by MTT and Comet assay. Results showed that at low concentration, up to 25 μg/mL, the cytotoxic effect was not observed. Increasing concentration from 50 to 75 μg/mL reduced significantly cell viability and induced an important DNA damage in hepatocellular carcinoma. These promising data were also confirmed with mitochondrial potential test. It is possible to conclude that E. angustifolium has beneficial properties in low concentration, in term of antioxidant activity, and it could be a potential antitumoral natural product if it will be used at high concentration

Otwarty dostęp

Clinical and economic issues complicating cost-effectiveness evaluation of orphan diseases

Data publikacji: 09 May 2017
Zakres stron: 132 - 137

Abstrakt

Abstract

Cost-effectiveness evaluation of orphan medicinal products is confronted with a large confidence interval on the incremental cost-effectiveness ratios (ICERs), or extremely high ICERs and therefore rejection of products for uptake in the health insurance package (coverage) by health authorities in Europe. Examples from the United Kingdom (UK) and The Netherlands illustrated that straightforward application of the decision criteria might not always be possible, resulting in a large variety of coverage decisions that were neither transparent nor consistent with the criteria. This observation required more insight into what drives the high ICERs and what policies may support the appropriate use of orphan medicinal products. The most relevant clinical and economic issues that are perceived to complicate the cost-effectiveness evaluation of orphan medicinal products are discussed. Theoretically, two possible solutions are available: 1) circumvent or 2) keep the standard assessment criterion costeffectiveness. In analogy to the Europe Medicine Agency (EMA) registration approach of orphan medicinal products that are hampered by limitations in the clinical data at the time of registration, we suggest to stick to the use of standard uniform criteria, but that efforts should be directed at optimising the input to the cost-effectiveness evaluation. Subsequently potential policy approaches are developed.

Otwarty dostęp

RHAMM-target peptides inhibit invasion of breast cancer cells

Data publikacji: 09 May 2017
Zakres stron: 138 - 148

Abstrakt

Abstract

RHAMM is hyaluronan- receptor with multiple functions in the cell, RHAMM is involved in proliferation, motility, migration, invasion, mitotic spindle formation in tumour cells. Therefore, RHAMM could be a relevant target for molecular targeted therapies against tumors.The role of RHAMM-target peptides in inhibition invasion for preventing breast cancer has not yet been investigated. Base on this, we analyzed the RHAMM-target peptides for their therapeutic activity against breast cancer cells. In the present study, we examined the effect of RHAMM-target peptides on the invasion of breast cancer cells (MDAMB- 231), using confocal microscopy. We shown that RHAMM-target peptides decreased formation of invadopodia of breast cancer cells. The treatment of breast cancer cells by RHAMM -target peptides inhibited the invasion up to 99 %. Additionally, RHAMM-target peptides induced the morphological changes of of breast cancer cells. Therefore, based on these results, we can conclude that RHAMM-target peptides may be potential anti-cancer agents.

Otwarty dostęp

Isolation and culture of endothelial cells from embryonic rat yolk sac

Data publikacji: 09 May 2017
Zakres stron: 149 - 154

Abstrakt

Abstract

Yolk sac blood islands are the first morphologic evidence of hematopoietic development during mammalian embryogenesis, and visseral yolk sac mesoderm gives rise to the first embryonic blood cells within a rich endothelial network. Present study reports the isolation and culture of endothelial cells from 11.5 days old embryonic rat yolk sac. The embryos were dissected from 11.5 days pregnant Wistar rat (Rattus norvegicus) and the external yolk sac membrane and embryos were removed under aseptic condition. After washing three times with Calcium-Magnesium free Hank’s balanced salt solution (CMF-HBSS), the tissue was minced, and fragments were incubated in CMF-HBSS containing 2mg/ml Trypsin, 100mg/ml collagenase I and 40mg/ml DNAse at 37°C until the tissue was completely dispersed. The digestion effect was then neutralized by fetal bovine serum at 1:3 (v/v). The cell suspension was centrifuged at 1000 rpm for 10 min., the supernatants were discarded and the cell pellets resuspended in Dulbecco modified Eagle medium containing 15% fetal bovine serum, 1.25mg/ml amphotericin B, 25mg/ml gentamycin sulphate and 100mg/ml endothelial cell growth supplement. The resuspended cells were plated in two diverse 25cm2 culture flasks for overnight differential adherence at 37°C. The non-adherent cells were removed by gentle aspiration and adherent cells refed with fresh medium. The cells were transferred using 1ml of 0.2% Trypsin when cultures reached near-confluence. The cultured yolk sac endothelial cells had characteristic cobblestone appearence and positive immunofluorescent staining for von Willebrand Factor (vWF). Weibel-Palade bodies, the major ultrastructural marker for endothelium, were also detected in cultured cells by electron microscopy.

Otwarty dostęp

Localization of nuclear actin in nuclear lipid microdomains of liver and hepatoma cells: Possible involvement of sphingomyelin metabolism

Data publikacji: 09 May 2017
Zakres stron: 155 - 158

Abstrakt

Abstract

Nuclear actin has been implicated in different nuclear functions. In this work, its localization in nuclear membrane, chromatin and nuclear lipid microdomains was investigated. The implication of sphingomyelin metabolism was studied. Nuclear membrane, chromatin and nuclear lipid microdomains were purified from hepatocyte nuclei and H35 human hepatoma cell nuclei. The presence of β-actin was analyzed with immunoblotting by using specific antibodies. Sphingomyelinase, sphingomyelin-synthase, and phosphatidylcholine-specific phospholipase C activities were assayed by using radioactivity sphingomyelin and phosphatidylcholine as substrate. The results showed that β-actin is localized in nuclear lipid microdomains and it increases in cancer cells. Evidence is provided to the difference of phosphatidylcholine and sphingomyelin metabolism in various subnuclear fractions of cancer cell nuclei compared with normal cells. Our findings show increase of sphingomyelin-synthase and inhibition of sphingomyelinase activity only in nuclear lipid microdomains. Nuclear lipid microdomains, constituted by phosphatidylcholine, sphingomyelin and cholesterol, play a role as platform for β-actin anchoring. Possible role of sphingomyelin metabolism in cancer cells is discussed.

Otwarty dostęp

Early phase value scan for biotechnology innovation

Data publikacji: 09 May 2017
Zakres stron: 159 - 164

Abstrakt

Abstract

The registration of a medicinal product by EMA or FDA used to be the main driving critical success factor for the future sales of a new medicinal product. The current additional important criteria for reimbursement decisions are cost-effectiveness and budgetary impact, which are taken into consideration in order to make a value for money decision. In order to maximize the chances of obtaining reimbursement at a maximum price, it is very important to have a well thought through strategy at the early onset of the development program in order to proactively cope with the emerging reimbursement hurdles. This paper aims to provide a pricing, market access and reimbursement strategy, which is based on a strategic scan, sales forecast model, pricing model, and cost-effectiveness model. These models are interacted and linked with a discounted cash flow model in order to optimize the economic value of the company.

Otwarty dostęp

Electrochemical impedimetric detection of stroke biomarker NT-proBNP using disposable screen-printed gold electrodes

Data publikacji: 09 May 2017
Zakres stron: 165 - 176

Abstrakt

Abstract

Stroke is the second top leading cause of death globally. It is caused by an abrupt interruption of blood flow to the brain. In that course, brain natriuretic peptide (BNP) and its derivative N-terminal pro b-type natriuretic peptide (NT-proBNP), neurohormones produced mainly by the heart ventricles in response to excessive stretching of cardiomyocytes (heart muscle cells), are proven to be good biomarkers for heart failure diagnosis. Moreover, there is growing clinical interest of the use of NT-proBNP for stroke diagnosis and prognosis because it is significantly associated with cardioembolic stroke and secondary stroke reoccurrence, with sensitivity >90% and specificity >80%. However, in diagnostic settings, there is still a need to address the encountered analytical problems, particularly assay specificity and set up. In this study, a novel approach for NT-proBNP detection is demonstrated using an electrochemical immunoassay method. A label-free impedimetry immunosensor for stroke biomarker was developed using modified disposable screen-printed gold electrodes (SPGE) hosting specific anti-NT-proBNP capture antibody. The performance of our immunosensor was studied in the presence of NT-proBNP in both buffered and mock (porcine) plasma samples. A linear relation between the relative total resistance (ΔRtot) responses and the NT-proBNP concentrations in buffer was observed in a range from 0.1 to 5 ng mL-1 with a correlation coefficient (R2) of 0.94656. Overall, the biosensor has demonstrated the capability to quantitate NT-proBNP and differentiate such concentrations in a low concentration range, especially among 0, 0.1, 0.5, 1, and 3 ng mL-1 in plasma samples within 25 min. This range is valuable not only for classifying cardioembolic stroke (higher or equal to 0.5 ng mL-1), but also predicting the risk of secondary stroke reoccurrence (higher than 0.255 ng mL-1). Our biosensor has the potential to be used as an easy-to-use point-of-care test that is both accurate and affordable.

Otwarty dostęp

Analysis of public RNA-seq data in studies of flax fiber biogenesis

Data publikacji: 09 May 2017
Zakres stron: 177 - 179

Abstrakt

Abstract

In this work we used publicly available raw RNA-seq data to elucidate mechanisms of flax fiber biogenesis by measuring expression of cell-wall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stem of flax (Linum usitatissimum cv. Bethune). Using public RNA-sequence data we have quantified and characterised the expression of the specific cell-wall genes in the top, middle and bottom parts of the Bethune flax stem. The most prominent findings are: Secondary cell-wall cellulose synthase (CesA) genes are expressed differentially in phloem and xylem in all parts of Bethune stem, in contrast with primary cell-wall cellulose synthase genes. Total expression level of cellulose synthase-like (Csl) genes is tissue invariant (although, CslG and CslE are differentially expressed) and smaller than the total expression of cellulose synthase genes. The CslD2D3 subgroup dominates total expression of CslD genes in both xylem and phloem. Expression levels of all expressed chitinase-like (Ctl) genes are tissue dependent in all parts of stem. Total expression level of chitinase-like genes is much higher than expression of cellulose synthase and cellulose synthase-like genes in both tissues.

Otwarty dostęp

Combined approach to analysis of protein-protein interactions

Data publikacji: 09 May 2017
Zakres stron: 180 - 181

Abstrakt

Abstract

In this work combined approach for protein-protein interactions analysis is described.

Otwarty dostęp

Evaluation of probiotic potential of Bifidobacterium animalis subsp. lactis strains: an in vitro study

Data publikacji: 09 May 2017
Zakres stron: 182 - 183

Abstrakt

Abstract

Probiotic potential of two bifidobacterial strains isolated from feces of healthy adults and identified as Bifidobacterium animalis subsp. lactis was evaluated using in vitro testing. The analyzed strains were able to ferment a broad spectrum of carbohydrates, produced bioactive exopolysaccharides, demonstrated high survival rate in model GIT conditions, under heat and oxidative stresses, inhibited growth of a wide range of pathogenic and opportunistic bacteria, and proved to be safe for biotechnological application. Based on the complex phenotypic characteristics tested, Bifidobacterium animalis subsp. lactis may be regarded as prospective probiotic cultures.

Otwarty dostęp

Study of genetic variability of Ribes L. representatives grown in Belarus

Data publikacji: 09 May 2017
Zakres stron: 184 - 187

Abstrakt

Abstract

The research aims to study the genetic variability of Ribes L. representatives grown in Belarus, and to reveal a set of DNAmarkers for its DNA-identification. We formed a set of SSR-markers that possess rather high diagnostic value and allow identifying of black currant and gooseberry varieties at a molecular level and can be recommended for DNA-identification of those cultures.

Otwarty dostęp

Allelic composition of the TaCwi-A1 and TaSus2-2В genes affecting grain weight in the collection of winter wheat cultivars

Data publikacji: 09 May 2017
Zakres stron: 188 - 191

Abstrakt

Abstract

Grain yield is closely associated with kernel weight. Cell wall invertase (CWI) and sucrose synthase (SUS) are one of the most important enzymes for sink tissue development and carbon partition, and has a high association with kernel weight. Allellic composition of the TaCwi-A1 and TaSus2-2В loci was tested in 79 winter wheat cultivars using a co-dominant markers CWI21- CWI22, which amplified 404 or 402-bp and Sus2-185/589H2- Sus2-227/589L2, which amplified 423 or 381-bp fragments in different wheat accessions respectively. Some samples carried the mutation in the TaCwi-A1 locus that negatively affects thousand-kernel weight (TKW) were shown to have TKW higher than the cultivars and lines that do not have this mutation in their genomes and despite the significant differences in TKW (from 39,4 to 59,8 g), all investigated varieties possess Hap- L haplotype. It can be attributed to the fact that the TaCwi-A1 and TaSus2-2В are only two of the genes associated with kernel weight and its allelic composition analysis cannot explain all phenotypic variances.

Otwarty dostęp

Cell wall gene expression in two sub-species of flax

Data publikacji: 09 May 2017
Zakres stron: 192 - 195

Abstrakt

Abstract

In this work, we attempted to elucidate mechanisms of flax fiber biogenesis by measuring, using qPCR, expression of the cellwall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stems of two contrasting fiber quality subspecies of flax (Linum usitatissimum L.). We studied elongatum Vav. et Ell. (cultivar Blakit, Belarus) and crepitans Boenn. (dehiscent flax) subspecies, which are differed in the height of plants, width of stems etc. Amongst all measured genes chitinase- like Ctl19 and Ctl21 genes showed drastic difference in expression between stems of the two flax varieties, showing higher expression level in the fiber flax versus the dehiscent flax. In contrast, cellulose synthase-like CslG4 gene had lower expression levels in the stem of fiber flax than in dehiscent flax. We suggest that hemicellulose composition and cellulose - non-cellulose glycan organisation can vary between stalk cells of different flax subspecies.

Otwarty dostęp

Testing for the presence of potato pathogens of viral, bacterial and viroid diseases

Data publikacji: 09 May 2017
Zakres stron: 196 - 197

Abstrakt

Abstract

The article considers a complex method of selecting parental lines, which significantly reduces the time of selection of parental lines and their introduction into culture.

Otwarty dostęp

Biotechnological importance of sphagnum mosses

Data publikacji: 09 May 2017
Zakres stron: 198 - 199

Abstrakt

Abstract

Since Belarus is rich in sphagnum bogs it is extremely important to develop ways to use the resources of sphagnum mosses with a number of useful properties. Sphagnum is the only genus in the class Sphagnopsida and includes 36 Bryophyta species in Belarus. Sphagnum mosses, which are widespread in Belarus, have great potential for possible use in contaminated water purification from various hydrophilic and hydrophobic solvents and salt solutions, in light industry and agriculture (considering the need of their renewal).

Otwarty dostęp

Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

Data publikacji: 09 May 2017
Zakres stron: 200 - 201

Abstrakt

Abstract

We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.

18 Artykułów
Otwarty dostęp

Ricinus communis L. (Castor bean), a potential multi-purpose environmental crop for improved and integrated phytoremediation

Data publikacji: 09 May 2017
Zakres stron: 101 - 116

Abstrakt

Abstract

Phytoremediation is a plant based environmental cleanup technology to contain (rendering less toxic), sequester and degrade contaminated susbtrates. As can be seen from data metrics, it is gaining cosiderable importance globally. Phytoremediation approach is being applied for cleanup of inorganic (potentially toxic metals), organic (persistent, emergent, poly-acromatic hydrocarbons and crude oil etc.) and co-contaminated (mixture of inorganic and organic) and/or polluted sites globally. Recently new approaches of utilizing abundantly available natural organic amendments have yielded significant results. Ricinus communis L. (Castor bean) is an important multipurpose crop viz., Agricultural, Energy, Environmental and Industrial crop. The current status of knowledge is abundant but scattered which need to be exploited for sustainable development. This review collates and evaluates all the scattered information and provides a critical view on the possible options for exploiting its potential as follows: 1. Origin and distribution, 2. Lead toxicity bioassays, 3. Progress in arbuscular mycorrhizal fungi-assisted phytoremediation, 4. Promising bioenergy crop that can be linked to pytoremediation, 5. A renewable source for many bioproducts with rich chemical diversity, 6. It is a good biomonitor and bioindicator of atmospheric pollution in urban areas, 7. Enhanced chelate aided remediation, 8. Its rhizospheric processes accelerate natural attenuation, 9. It is suitable for remediation of crude oil contaminated soil, 10. It is an ideal candidate for aided phytostabilization, 11. Castor bean is a wizard for phytoremediation and 12. Its use in combined phytoextraction and ecocatalysis. Further, the knowledge gaps and scope for future research on sustainable co-generation of value chain and value addition biobased products for sustainable circular economy and environmental security are described in this paper.

Otwarty dostęp

Influence of OASL gene polymorphisms on host response to interferon therapy in chronic hepatitis C virus patients

Data publikacji: 09 May 2017
Zakres stron: 117 - 125

Abstrakt

Abstract

Hepatitis C virus (HCV) infection becomes a major public health problem and leading cause of chronic liver disease and liver failure. Pegylated interferon-alfa and ribavirin are currently the standard treatment for chronic hepatitis C (CHC). It is indicated that genes that trace the interferon signaling could be associated with the host response to the therapy. In order to investigate the influence of these genes on host related response, we have analyzed seven single nucleotide polymorphisms (rs59248852, rs74390571, rs12811390, rs1169279, rs3213545, rs1083129 and rs2859398) in 2-5-Oligoadenylate- Synthetase-Like (OASL) gene in CHC cases from Republic of Macedonia. A simple and easy to use SNaPshot method was developed. A cohort of 100 HCV RNA positive patients - non responders and 109 patients with achieved virological response after the standard treatment were included in this study. We have found significant association in five of the seven studied SNP` s: rs59248852 [p = 6.5x10-31, OR=55.7 (20.0-155.3)]; rs12811390 [p = 2.2x10-11, OR=4.3 (2.3-6.7)]; rs2859398 [p=1.34x10-9, OR=3.4 (2.2-5.0)]; rs74390571 [p=4.3x10-7, OR=2.9 (1.9-4.4)], and rs1083129 [p=0.0139, OR=2.0 (1.1-3.5)]. The results from this study can be used as a predictive factor of future patient’s selection for the standard therapy, having an important cost benefit for the health insurance system.

Otwarty dostęp

Epilobium angustifolium L.: A medicinal plant with therapeutic properties

Data publikacji: 09 May 2017
Zakres stron: 126 - 131

Abstrakt

Abstract

Epilobium angustifolium L. is a medicinal plant belonging to the Onagraceae family, which includes more than 200 different species from all over the world. Traditional medicinal applications include treatment of prostate, gastrointestinal, menstrual disorders and recently it has been used for its analgesic and anti-inflammatory activity. In this investigation E. angustifolium was collected in Ternopil region of Ukraine. The obtained data demonstrated that E. angustifolium herb extract, rich in polyphenolic compounds such as flavonoids and tannins, display high antioxidant properties. In addition the potential anticancer activity has been investigated in vitro on human hepatocellular carcinoma cells (HepG2). Furthermore the cytotoxic and genotoxic effects of E. angustifolium have been investigated respectively by MTT and Comet assay. Results showed that at low concentration, up to 25 μg/mL, the cytotoxic effect was not observed. Increasing concentration from 50 to 75 μg/mL reduced significantly cell viability and induced an important DNA damage in hepatocellular carcinoma. These promising data were also confirmed with mitochondrial potential test. It is possible to conclude that E. angustifolium has beneficial properties in low concentration, in term of antioxidant activity, and it could be a potential antitumoral natural product if it will be used at high concentration

Otwarty dostęp

Clinical and economic issues complicating cost-effectiveness evaluation of orphan diseases

Data publikacji: 09 May 2017
Zakres stron: 132 - 137

Abstrakt

Abstract

Cost-effectiveness evaluation of orphan medicinal products is confronted with a large confidence interval on the incremental cost-effectiveness ratios (ICERs), or extremely high ICERs and therefore rejection of products for uptake in the health insurance package (coverage) by health authorities in Europe. Examples from the United Kingdom (UK) and The Netherlands illustrated that straightforward application of the decision criteria might not always be possible, resulting in a large variety of coverage decisions that were neither transparent nor consistent with the criteria. This observation required more insight into what drives the high ICERs and what policies may support the appropriate use of orphan medicinal products. The most relevant clinical and economic issues that are perceived to complicate the cost-effectiveness evaluation of orphan medicinal products are discussed. Theoretically, two possible solutions are available: 1) circumvent or 2) keep the standard assessment criterion costeffectiveness. In analogy to the Europe Medicine Agency (EMA) registration approach of orphan medicinal products that are hampered by limitations in the clinical data at the time of registration, we suggest to stick to the use of standard uniform criteria, but that efforts should be directed at optimising the input to the cost-effectiveness evaluation. Subsequently potential policy approaches are developed.

Otwarty dostęp

RHAMM-target peptides inhibit invasion of breast cancer cells

Data publikacji: 09 May 2017
Zakres stron: 138 - 148

Abstrakt

Abstract

RHAMM is hyaluronan- receptor with multiple functions in the cell, RHAMM is involved in proliferation, motility, migration, invasion, mitotic spindle formation in tumour cells. Therefore, RHAMM could be a relevant target for molecular targeted therapies against tumors.The role of RHAMM-target peptides in inhibition invasion for preventing breast cancer has not yet been investigated. Base on this, we analyzed the RHAMM-target peptides for their therapeutic activity against breast cancer cells. In the present study, we examined the effect of RHAMM-target peptides on the invasion of breast cancer cells (MDAMB- 231), using confocal microscopy. We shown that RHAMM-target peptides decreased formation of invadopodia of breast cancer cells. The treatment of breast cancer cells by RHAMM -target peptides inhibited the invasion up to 99 %. Additionally, RHAMM-target peptides induced the morphological changes of of breast cancer cells. Therefore, based on these results, we can conclude that RHAMM-target peptides may be potential anti-cancer agents.

Otwarty dostęp

Isolation and culture of endothelial cells from embryonic rat yolk sac

Data publikacji: 09 May 2017
Zakres stron: 149 - 154

Abstrakt

Abstract

Yolk sac blood islands are the first morphologic evidence of hematopoietic development during mammalian embryogenesis, and visseral yolk sac mesoderm gives rise to the first embryonic blood cells within a rich endothelial network. Present study reports the isolation and culture of endothelial cells from 11.5 days old embryonic rat yolk sac. The embryos were dissected from 11.5 days pregnant Wistar rat (Rattus norvegicus) and the external yolk sac membrane and embryos were removed under aseptic condition. After washing three times with Calcium-Magnesium free Hank’s balanced salt solution (CMF-HBSS), the tissue was minced, and fragments were incubated in CMF-HBSS containing 2mg/ml Trypsin, 100mg/ml collagenase I and 40mg/ml DNAse at 37°C until the tissue was completely dispersed. The digestion effect was then neutralized by fetal bovine serum at 1:3 (v/v). The cell suspension was centrifuged at 1000 rpm for 10 min., the supernatants were discarded and the cell pellets resuspended in Dulbecco modified Eagle medium containing 15% fetal bovine serum, 1.25mg/ml amphotericin B, 25mg/ml gentamycin sulphate and 100mg/ml endothelial cell growth supplement. The resuspended cells were plated in two diverse 25cm2 culture flasks for overnight differential adherence at 37°C. The non-adherent cells were removed by gentle aspiration and adherent cells refed with fresh medium. The cells were transferred using 1ml of 0.2% Trypsin when cultures reached near-confluence. The cultured yolk sac endothelial cells had characteristic cobblestone appearence and positive immunofluorescent staining for von Willebrand Factor (vWF). Weibel-Palade bodies, the major ultrastructural marker for endothelium, were also detected in cultured cells by electron microscopy.

Otwarty dostęp

Localization of nuclear actin in nuclear lipid microdomains of liver and hepatoma cells: Possible involvement of sphingomyelin metabolism

Data publikacji: 09 May 2017
Zakres stron: 155 - 158

Abstrakt

Abstract

Nuclear actin has been implicated in different nuclear functions. In this work, its localization in nuclear membrane, chromatin and nuclear lipid microdomains was investigated. The implication of sphingomyelin metabolism was studied. Nuclear membrane, chromatin and nuclear lipid microdomains were purified from hepatocyte nuclei and H35 human hepatoma cell nuclei. The presence of β-actin was analyzed with immunoblotting by using specific antibodies. Sphingomyelinase, sphingomyelin-synthase, and phosphatidylcholine-specific phospholipase C activities were assayed by using radioactivity sphingomyelin and phosphatidylcholine as substrate. The results showed that β-actin is localized in nuclear lipid microdomains and it increases in cancer cells. Evidence is provided to the difference of phosphatidylcholine and sphingomyelin metabolism in various subnuclear fractions of cancer cell nuclei compared with normal cells. Our findings show increase of sphingomyelin-synthase and inhibition of sphingomyelinase activity only in nuclear lipid microdomains. Nuclear lipid microdomains, constituted by phosphatidylcholine, sphingomyelin and cholesterol, play a role as platform for β-actin anchoring. Possible role of sphingomyelin metabolism in cancer cells is discussed.

Otwarty dostęp

Early phase value scan for biotechnology innovation

Data publikacji: 09 May 2017
Zakres stron: 159 - 164

Abstrakt

Abstract

The registration of a medicinal product by EMA or FDA used to be the main driving critical success factor for the future sales of a new medicinal product. The current additional important criteria for reimbursement decisions are cost-effectiveness and budgetary impact, which are taken into consideration in order to make a value for money decision. In order to maximize the chances of obtaining reimbursement at a maximum price, it is very important to have a well thought through strategy at the early onset of the development program in order to proactively cope with the emerging reimbursement hurdles. This paper aims to provide a pricing, market access and reimbursement strategy, which is based on a strategic scan, sales forecast model, pricing model, and cost-effectiveness model. These models are interacted and linked with a discounted cash flow model in order to optimize the economic value of the company.

Otwarty dostęp

Electrochemical impedimetric detection of stroke biomarker NT-proBNP using disposable screen-printed gold electrodes

Data publikacji: 09 May 2017
Zakres stron: 165 - 176

Abstrakt

Abstract

Stroke is the second top leading cause of death globally. It is caused by an abrupt interruption of blood flow to the brain. In that course, brain natriuretic peptide (BNP) and its derivative N-terminal pro b-type natriuretic peptide (NT-proBNP), neurohormones produced mainly by the heart ventricles in response to excessive stretching of cardiomyocytes (heart muscle cells), are proven to be good biomarkers for heart failure diagnosis. Moreover, there is growing clinical interest of the use of NT-proBNP for stroke diagnosis and prognosis because it is significantly associated with cardioembolic stroke and secondary stroke reoccurrence, with sensitivity >90% and specificity >80%. However, in diagnostic settings, there is still a need to address the encountered analytical problems, particularly assay specificity and set up. In this study, a novel approach for NT-proBNP detection is demonstrated using an electrochemical immunoassay method. A label-free impedimetry immunosensor for stroke biomarker was developed using modified disposable screen-printed gold electrodes (SPGE) hosting specific anti-NT-proBNP capture antibody. The performance of our immunosensor was studied in the presence of NT-proBNP in both buffered and mock (porcine) plasma samples. A linear relation between the relative total resistance (ΔRtot) responses and the NT-proBNP concentrations in buffer was observed in a range from 0.1 to 5 ng mL-1 with a correlation coefficient (R2) of 0.94656. Overall, the biosensor has demonstrated the capability to quantitate NT-proBNP and differentiate such concentrations in a low concentration range, especially among 0, 0.1, 0.5, 1, and 3 ng mL-1 in plasma samples within 25 min. This range is valuable not only for classifying cardioembolic stroke (higher or equal to 0.5 ng mL-1), but also predicting the risk of secondary stroke reoccurrence (higher than 0.255 ng mL-1). Our biosensor has the potential to be used as an easy-to-use point-of-care test that is both accurate and affordable.

Otwarty dostęp

Analysis of public RNA-seq data in studies of flax fiber biogenesis

Data publikacji: 09 May 2017
Zakres stron: 177 - 179

Abstrakt

Abstract

In this work we used publicly available raw RNA-seq data to elucidate mechanisms of flax fiber biogenesis by measuring expression of cell-wall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stem of flax (Linum usitatissimum cv. Bethune). Using public RNA-sequence data we have quantified and characterised the expression of the specific cell-wall genes in the top, middle and bottom parts of the Bethune flax stem. The most prominent findings are: Secondary cell-wall cellulose synthase (CesA) genes are expressed differentially in phloem and xylem in all parts of Bethune stem, in contrast with primary cell-wall cellulose synthase genes. Total expression level of cellulose synthase-like (Csl) genes is tissue invariant (although, CslG and CslE are differentially expressed) and smaller than the total expression of cellulose synthase genes. The CslD2D3 subgroup dominates total expression of CslD genes in both xylem and phloem. Expression levels of all expressed chitinase-like (Ctl) genes are tissue dependent in all parts of stem. Total expression level of chitinase-like genes is much higher than expression of cellulose synthase and cellulose synthase-like genes in both tissues.

Otwarty dostęp

Combined approach to analysis of protein-protein interactions

Data publikacji: 09 May 2017
Zakres stron: 180 - 181

Abstrakt

Abstract

In this work combined approach for protein-protein interactions analysis is described.

Otwarty dostęp

Evaluation of probiotic potential of Bifidobacterium animalis subsp. lactis strains: an in vitro study

Data publikacji: 09 May 2017
Zakres stron: 182 - 183

Abstrakt

Abstract

Probiotic potential of two bifidobacterial strains isolated from feces of healthy adults and identified as Bifidobacterium animalis subsp. lactis was evaluated using in vitro testing. The analyzed strains were able to ferment a broad spectrum of carbohydrates, produced bioactive exopolysaccharides, demonstrated high survival rate in model GIT conditions, under heat and oxidative stresses, inhibited growth of a wide range of pathogenic and opportunistic bacteria, and proved to be safe for biotechnological application. Based on the complex phenotypic characteristics tested, Bifidobacterium animalis subsp. lactis may be regarded as prospective probiotic cultures.

Otwarty dostęp

Study of genetic variability of Ribes L. representatives grown in Belarus

Data publikacji: 09 May 2017
Zakres stron: 184 - 187

Abstrakt

Abstract

The research aims to study the genetic variability of Ribes L. representatives grown in Belarus, and to reveal a set of DNAmarkers for its DNA-identification. We formed a set of SSR-markers that possess rather high diagnostic value and allow identifying of black currant and gooseberry varieties at a molecular level and can be recommended for DNA-identification of those cultures.

Otwarty dostęp

Allelic composition of the TaCwi-A1 and TaSus2-2В genes affecting grain weight in the collection of winter wheat cultivars

Data publikacji: 09 May 2017
Zakres stron: 188 - 191

Abstrakt

Abstract

Grain yield is closely associated with kernel weight. Cell wall invertase (CWI) and sucrose synthase (SUS) are one of the most important enzymes for sink tissue development and carbon partition, and has a high association with kernel weight. Allellic composition of the TaCwi-A1 and TaSus2-2В loci was tested in 79 winter wheat cultivars using a co-dominant markers CWI21- CWI22, which amplified 404 or 402-bp and Sus2-185/589H2- Sus2-227/589L2, which amplified 423 or 381-bp fragments in different wheat accessions respectively. Some samples carried the mutation in the TaCwi-A1 locus that negatively affects thousand-kernel weight (TKW) were shown to have TKW higher than the cultivars and lines that do not have this mutation in their genomes and despite the significant differences in TKW (from 39,4 to 59,8 g), all investigated varieties possess Hap- L haplotype. It can be attributed to the fact that the TaCwi-A1 and TaSus2-2В are only two of the genes associated with kernel weight and its allelic composition analysis cannot explain all phenotypic variances.

Otwarty dostęp

Cell wall gene expression in two sub-species of flax

Data publikacji: 09 May 2017
Zakres stron: 192 - 195

Abstrakt

Abstract

In this work, we attempted to elucidate mechanisms of flax fiber biogenesis by measuring, using qPCR, expression of the cellwall related genes (cellulose synthase, cellulose synthase-like and chitinase-like genes) in stems of two contrasting fiber quality subspecies of flax (Linum usitatissimum L.). We studied elongatum Vav. et Ell. (cultivar Blakit, Belarus) and crepitans Boenn. (dehiscent flax) subspecies, which are differed in the height of plants, width of stems etc. Amongst all measured genes chitinase- like Ctl19 and Ctl21 genes showed drastic difference in expression between stems of the two flax varieties, showing higher expression level in the fiber flax versus the dehiscent flax. In contrast, cellulose synthase-like CslG4 gene had lower expression levels in the stem of fiber flax than in dehiscent flax. We suggest that hemicellulose composition and cellulose - non-cellulose glycan organisation can vary between stalk cells of different flax subspecies.

Otwarty dostęp

Testing for the presence of potato pathogens of viral, bacterial and viroid diseases

Data publikacji: 09 May 2017
Zakres stron: 196 - 197

Abstrakt

Abstract

The article considers a complex method of selecting parental lines, which significantly reduces the time of selection of parental lines and their introduction into culture.

Otwarty dostęp

Biotechnological importance of sphagnum mosses

Data publikacji: 09 May 2017
Zakres stron: 198 - 199

Abstrakt

Abstract

Since Belarus is rich in sphagnum bogs it is extremely important to develop ways to use the resources of sphagnum mosses with a number of useful properties. Sphagnum is the only genus in the class Sphagnopsida and includes 36 Bryophyta species in Belarus. Sphagnum mosses, which are widespread in Belarus, have great potential for possible use in contaminated water purification from various hydrophilic and hydrophobic solvents and salt solutions, in light industry and agriculture (considering the need of their renewal).

Otwarty dostęp

Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

Data publikacji: 09 May 2017
Zakres stron: 200 - 201

Abstrakt

Abstract

We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.

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