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A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic Microbes

Masataka Aoki
Masataka Aoki
, 
Takuya Noma
Takuya Noma
, 
Hiroshi Yonemitsu
Hiroshi Yonemitsu
, 
Nobuo Araki
Nobuo Araki
, 
Takashi Yamaguchi
Takashi Yamaguchi
 oraz 
Kazuyuki Hayashi
Kazuyuki Hayashi
   | 21 lut 2022
Polish Journal of Microbiology's Cover Image
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Article Category: original-paper
Data publikacji: 21 lut 2022
Zakres stron: 59 - 65
Otrzymano: 16 maj 2017
Przyjęty: 27 wrz 2017
DOI: https://doi.org/10.5604/01.3001.0011.6144
Słowa kluczowe
© 2021 Masataka Aoki et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Schematic diagram of the down-flow hanging sponge bioreactor system used in this study.
Schematic diagram of the down-flow hanging sponge bioreactor system used in this study.

Fig. 2.

Time course changes in the potential urease activity of down-flow hanging sponge bioreactor enrichments.The standard errors of the mean of potential urease activity values (n = 3) were smaller than the size of the filed circles; therefore, they are not shown.
Time course changes in the potential urease activity of down-flow hanging sponge bioreactor enrichments.The standard errors of the mean of potential urease activity values (n = 3) were smaller than the size of the filed circles; therefore, they are not shown.

Fig. 3.

Time-course changes in the pH (●) and oxidation-reduction potential (ORP; ☐) values of the effluent from the down-flow hanging sponge bioreactor.
Time-course changes in the pH (●) and oxidation-reduction potential (ORP; ☐) values of the effluent from the down-flow hanging sponge bioreactor.

Fig. 4.

Scatter plot of potential urease activities and biomass concentrations observed in the down-flow hanging sponge bioreactor enrichments.The numbers associated with the filled circles are the sampling times for the bioreactor enrichments. The error bars are the standard error of the mean (n = 3), and filed circle symbols without error bars have standard errors that are smaller than the size of the circle.
Scatter plot of potential urease activities and biomass concentrations observed in the down-flow hanging sponge bioreactor enrichments.The numbers associated with the filled circles are the sampling times for the bioreactor enrichments. The error bars are the standard error of the mean (n = 3), and filed circle symbols without error bars have standard errors that are smaller than the size of the circle.

Fig. 5.

Fluorescence in situ hybridization analysis.(A) and (B) Representative photomicrographs of a down-flow hanging sponge bioreactor enrichment collected on day 130 after in situ hybridization with the phylum Firmicutes-targeted LGC354mix probe. Photomicrographs of DAPI-stained cells (A) and the LGC354mix-stained cells (B) in an identical field. Scale bars = 10 μm [in (A) and (B)]. (C) Detection rates (% DAPI-stained cells) of EUB338mix and LGC354mix probe-targeted microbial populations in down-flow hanging sponge bioreactor enrichments. The error bars in (C) are the standard error of the mean from 10 different microscopic fields.
Fluorescence in situ hybridization analysis.(A) and (B) Representative photomicrographs of a down-flow hanging sponge bioreactor enrichment collected on day 130 after in situ hybridization with the phylum Firmicutes-targeted LGC354mix probe. Photomicrographs of DAPI-stained cells (A) and the LGC354mix-stained cells (B) in an identical field. Scale bars = 10 μm [in (A) and (B)]. (C) Detection rates (% DAPI-stained cells) of EUB338mix and LGC354mix probe-targeted microbial populations in down-flow hanging sponge bioreactor enrichments. The error bars in (C) are the standard error of the mean from 10 different microscopic fields.

Fig. 6.

Mineralogy and surface morphology of precipitates obtained by a down-flow hanging sponge bioreactor enrichment collected on day 105.(A) X-ray diffraction pattern of the induced precipitates. The peaks marked with filled circles arose from calcite. (B)-(D) Representative scanning electron microscopy images of the induced precipitates. (B) Dodecahedron-like crystals. (C) A cluster of hexahedral-like crystals. (D) A cluster of irregular-shaped crystals. Scale bar = 10 μm [in (B)−(D)].
Mineralogy and surface morphology of precipitates obtained by a down-flow hanging sponge bioreactor enrichment collected on day 105.(A) X-ray diffraction pattern of the induced precipitates. The peaks marked with filled circles arose from calcite. (B)-(D) Representative scanning electron microscopy images of the induced precipitates. (B) Dodecahedron-like crystals. (C) A cluster of hexahedral-like crystals. (D) A cluster of irregular-shaped crystals. Scale bar = 10 μm [in (B)−(D)].
eISSN:
2544-4646
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Microbiology and Virology
Kanał RSS czasopisma