Antimicrobial Resistance Mechanisms in Carbapenem-Resistant Pseudomonas aeruginosa Clinical Strains Isolated in Shanghai, China

Abaza AF, El Shazly SA, Selim HSA, Aly GSA. Metallo-beta-lactamase producing Pseudomonas aeruginosa in a healthcare setting in Alexandria, Egypt. Pol J Microbiol. 2017 Sep;66(3):297–308. https://doi.org/10.5604/01.3001.0010.4855 Abaza AF El Shazly SA Selim HSA Aly GSA. Metallo-beta-lact-amase producing Pseudomonas aeruginosa in a healthcare setting in Alexandria, Egypt . Pol J Microbiol . 2017 Sep ; 66 ( 3 ): 297 – 308 . https://doi.org/10.5604/01.3001.0010.4855 Search in Google Scholar

Alav I, Sutton JM, Rahman KM. Role of bacterial efflux pumps in biofilm formation. J Antimicrob Chemother. 2018 Aug;73(8):2003–2020. https://doi.org/10.1093/jac/dky042 Alav I Sutton JM Rahman KM. Role of bacterial efflux pumps in biofilm formation . J Antimicrob Chemother . 2018 Aug ; 73 ( 8 ): 2003 – 2020 . https://doi.org/10.1093/jac/dky042 Search in Google Scholar

Alcock BP, Raphenya AR, Lau TTY, Tsang KK, Bouchard M, Edalatmand A, Huynh W, Nguyen AV, Cheng AA, Liu S, et al. CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Res. 2020 Jan;48(D1):D517–D525. https://doi.org/10.1093/nar/gkz935 Alcock BP Raphenya AR Lau TTY Tsang KK Bouchard M Edalatmand A Huynh W Nguyen AV Cheng AA Liu S CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database . Nucleic Acids Res . 2020 Jan ; 48 ( D1 ): D517 – D525 . https://doi.org/10.1093/nar/gkz935 Search in Google Scholar

Avakh A, Grant GD, Cheesman MJ, Kalkundri T, Hall S. The art of war with Pseudomonas aeruginosa: Targeting Mex efflux pumps directly to strategically enhance antipseudomonal drug efficacy. Antibiotics. 2023;12(8):1304. https://doi.org/10.3390/antibiotics12081304 Avakh A Grant GD Cheesman MJ Kalkundri T Hall S. The art of war with Pseudomonas aeruginosa: Targeting Mex efflux pumps directly to strategically enhance antipseudomonal drug efficacy . Antibiotics . 2023 ; 12 ( 8 ): 1304 . https://doi.org/10.3390/antibiotics12081304 Search in Google Scholar

Bai Y, Gong YE, Shen F, Li H, Cheng Y, Guo J, Liu G, Ji AF. Molecular epidemiological characteristics of carbapenem-resistant Pseudomonas aeruginosa clinical isolates in southeast Shanxi, China. J Glob Antimicrob Resist. 2024 Mar;36:301–306. https://doi.org/10.1016/j.jgar.2023.12.029 Bai Y Gong YE Shen F Li H Cheng Y Guo J Liu G Ji AF. Molecular epidemiological characteristics of carbapenem-resistant Pseudomonas aeruginosa clinical isolates in southeast Shanxi, China . J Glob Antimicrob Resist . 2024 Mar ; 36 : 301 – 306 . https://doi.org/10.1016/j.jgar.2023.12.029 Search in Google Scholar

Bortolaia V, Kaas RS, Ruppe E, Roberts MC, Schwarz S, Cattoir V, Philippon A, Allesoe RL, Rebelo AR, Florensa AF, et al. ResFinder 4.0 for predictions of phenotypes from genotypes. J Antimicrob Chemother. 2020 Dec;75(12):3491–3500. https://doi.org/10.1093/jac/dkaa345 Bortolaia V Kaas RS Ruppe E Roberts MC Schwarz S Cattoir V Philippon A Allesoe RL Rebelo AR Florensa AF ResFinder 4.0 for predictions of phenotypes from genotypes . J Antimicrob Chemother . 2020 Dec ; 75 ( 12 ): 3491 – 3500 . https://doi.org/10.1093/jac/dkaa345 Search in Google Scholar

Cabrera R, Fernández-Barat L, Vázquez N, Alcaraz-Serrano V, Bueno-Freire L, Amaro R, López-Aladid R, Oscanoa P, Muñoz L, Vila J, et al. Resistance mechanisms and molecular epidemiology of Pseudomonas aeruginosa strains from patients with bronchiectasis. J Antimicrob Chemother. 2022 May;77(6):1600–1610. https://doi.org/10.1093/jac/dkac084 Cabrera R Fernández-Barat L Vázquez N Alcaraz-Serrano V Bueno-Freire L Amaro R López-Aladid R Oscanoa P Muñoz L Vila J Resistance mechanisms and molecular epidemiology of Pseudomonas aeruginosa strains from patients with bronchiectasis . J Antimicrob Chemother . 2022 May ; 77 ( 6 ): 1600 – 1610 . https://doi.org/10.1093/jac/dkac084 Search in Google Scholar

Chairat S, Ben Yahia H, Rojo-Bezares B, Sáenz Y, Torres C, Ben Slama K. High prevalence of imipenem-resistant and metallo-beta-lactamase-producing Pseudomonas aeruginosa in the Burns Hospital in Tunisia: Detection of a novel class 1 integron. J Chemother. 2019 May;31(3):120–126. https://doi.org/10.1080/1120009x.2019.1582168 Chairat S Ben Yahia H Rojo-Bezares B Sáenz Y Torres C Ben Slama K. High prevalence of imipenem-resistant and metallo-beta-lactamase-producing Pseudomonas aeruginosa in the Burns Hospital in Tunisia: Detection of a novel class 1 integron . J Chemother . 2019 May ; 31 ( 3 ): 120 – 126 . https://doi.org/10.1080/1120009x.2019.1582168 Search in Google Scholar

Chen S, Zhou Y, Chen Y, Gu J. fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018 Sep;34(17):i884–i890. https://doi.org/10.1093/bioinformatics/bty560 Chen S Zhou Y Chen Y Gu J. fastp: An ultra-fast all-in-one FASTQ preprocessor . Bioinformatics . 2018 Sep ; 34 ( 17 ): i884 – i890 . https://doi.org/10.1093/bioinformatics/bty560 Search in Google Scholar

CLSI. Performance standards for antimicrobial susceptibility testing. 31^st ed. CLSI supplement M100. Wayne (USA): Clinical and Laboratory Standards Institute; 2021. CLSI . Performance standards for antimicrobial susceptibility testing . 31^st ed. CLSI supplement M100 . Wayne (USA) : Clinical and Laboratory Standards Institute ; 2021 . Search in Google Scholar

Çopur Çiçek A, Ertürk A, Ejder N, Rakici E, Kostakoğlu U, Esen Yıldız İ, Özyurt S, Sönmez E. Screening of antimicrobial resistance genes and epidemiological features in hospital and community-associated carbapenem-resistant Pseudomonas aeruginosa infections. Infect Drug Resist. 2021 Apr;14:1517–1526. https://doi.org/10.2147/idr.s299742 Çopur Çiçek A Ertürk A Ejder N Rakici E Kostakoğlu U Esen Yıldız İ Özyurt S Sönmez E. Screening of antimicrobial resistance genes and epidemiological features in hospital and community-associated carbapenem-resistant Pseudomonas aeruginosa infections . Infect Drug Resist . 2021 Apr ; 14 : 1517 – 1526 . https://doi.org/10.2147/idr.s299742 Search in Google Scholar

de Paula-Petroli SB, Campana EH, Bocchi M, Bordinhão T, Picão RC, Yamada-Ogatta SF, Carrara-Marroni FE. Early detection of a hypervirulent KPC-2-producing Pseudomonas aeruginosa ST235 in Brazil. J Glob Antimicrob Resist. 2018 Mar;12:153–154. https://doi.org/10.1016/j.jgar.2018.01.014 de Paula-Petroli SB Campana EH Bocchi M Bordinhão T Picão RC Yamada-Ogatta SF Carrara-Marroni FE. Early detection of a hypervirulent KPC-2-producing Pseudomonas aeruginosa ST235 in Brazil . J Glob Antimicrob Resist . 2018 Mar ; 12 : 153 – 154 . https://doi.org/10.1016/j.jgar.2018.01.014 Search in Google Scholar

del Barrio-Tofiño E, López-Causapé C, Oliver A. Pseudomonas aeruginosa epidemic high-risk clones and their association with horizontally-acquired β-lactamases: 2020 update. Int J Antimicrob Agents. 2020 Dec;56(6):106196. https://doi.org/10.1016/j.ijantimi-cag.2020.106196 del Barrio-Tofiño E López-Causapé C Oliver A. Pseudomonas aeruginosa epidemic high-risk clones and their association with horizontally-acquired β-lactamases: 2020 update . Int J Antimicrob Agents . 2020 Dec ; 56 ( 6 ): 106196 . https://doi.org/10.1016/j.ijantimi-cag.2020.106196 Search in Google Scholar

Dos Santos PAS, Silva MJA, Gouveia MIM, Lima LNGC, Quaresma AJPG, De Lima PDL, Brasiliense DM, Lima KVB, Rodrigues YC. ‘I he prevalence of metallo-beta-lactamese-(MβL)-producing Pseudomonas aeruginosa isolates in Brazil: A Systematic Review and Meta-Analysis. Microorganisms. 2023 Sep;11(9):2366. https://doi.org/10.3390/microorganisms11092366 Dos Santos PAS Silva MJA Gouveia MIM Lima LNGC Quaresma AJPG De Lima PDL Brasiliense DM Lima KVB Rodrigues YC. ‘The prevalence of metallo-beta-lactamese-(MβL)-producing Pseudomonas aeruginosa isolates in Brazil: A Systematic Review and Meta-Analysis . Microorganisms . 2023 Sep ; 11 ( 9 ): 2366 . https://doi.org/10.3390/microorganisms11092366 Search in Google Scholar

Escudero JA, Loot C, Nivina A, Mazel D. The Integron: Adaptation on demand. Microbiol Spectr. 2015;3(2):MDNA3-2014. https://doi.org/10.1128/microbiolspec.MDNA3-0019-2014 Escudero JA Loot C Nivina A Mazel D. The Integron: Adaptation on demand . Microbiol Spectr . 2015 ; 3 ( 2 ): MDNA3 – 2014 . https://doi.org/10.1128/microbiolspec.MDNA3-0019-2014 Search in Google Scholar

Gadaime NK, Haddadin RN, Shehabi AA, Omran IN. Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: A call for surveillance and intervention. Libyan J Med. 2024 Dec;19(1):2344320. https://doi.org/10.1080/19932820.2024.2344320 Gadaime NK Haddadin RN Shehabi AA Omran IN. Antimicrobial resistance and carbapenemase dissemination in Pseudomonas aeruginosa isolates from Libyan hospitals: A call for surveillance and intervention . Libyan J Med . 2024 Dec ; 19 ( 1 ): 2344320 . https://doi.org/10.1080/19932820.2024.2344320 Search in Google Scholar

Girlich D, Naas T, Nordmann P. Biochemical characterization of the naturally occurring oxacillinase OXA-50 of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2004;48(6):2043–2048. https://doi.org/10.1128/aac.48.6.2043-2048.2004 Girlich D Naas T Nordmann P. Biochemical characterization of the naturally occurring oxacillinase OXA-50 of Pseudomonas aeruginosa . Antimicrob Agents Chemother . 2004 ; 48 ( 6 ): 2043 – 2048 . https://doi.org/10.1128/aac.48.6.2043-2048.2004 Search in Google Scholar

Goli HR, Nahaei MR, Rezaee MA, Hasani A, Samadi Kafil H, Aghazadeh M, Sheikhalizadeh V. Contribution of mexAB-oprM and mexXY (-oprA) efflux operons in antibiotic resistance of clinical Pseudomonas aeruginosa isolates in Tabriz, Iran. Infect Genet Evol. 2016 Nov;45:75–82. https://doi.org/10.1016/j.meegid.2016.08.022 Goli HR Nahaei MR Rezaee MA Hasani A Samadi Kafil H Aghazadeh M Sheikhalizadeh V. Contribution of mexAB-oprM and mexXY (-oprA) efflux operons in antibiotic resistance of clinical Pseudomonas aeruginosa isolates in Tabriz, Iran . Infect Genet Evol . 2016 Nov ; 45 : 75 – 82 . https://doi.org/10.1016/j.meegid.2016.08.022 Search in Google Scholar

González-Bello C, Rodríguez D, Pernas M, Rodríguez Á, Colchón E. β-Lactamase inhibitors to restore the efficacy of antibiotics against superbugs. J Med Chem. 2020 Mar;63(5):1859–1881. https://doi.org/10.1021/acs.jmedchem.9b01279 González-Bello C Rodríguez D Pernas M Rodríguez Á Colchón E. β-Lactamase inhibitors to restore the efficacy of antibiotics against superbugs . J Med Chem . 2020 Mar ; 63 ( 5 ): 1859 – 1881 . https://doi.org/10.1021/acs.jmedchem.9b01279 Search in Google Scholar

Hall BG, Nisbet J. Building phylogenetic trees from genome sequences with kSNP4. Mol Biol Evol. 2023 Nov;40(11):msad235. https://doi.org/10.1093/molbev/msad235 Hall BG Nisbet J. Building phylogenetic trees from genome sequences with kSNP4 . Mol Biol Evol . 2023 Nov ; 40 ( 11 ): msad235 . https://doi.org/10.1093/molbev/msad235 Search in Google Scholar

Horna G, López M, Guerra H, Saénz Y, Ruiz J. Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa. Sci Rep. 2018 Nov;8(1):16463. https://doi.org/10.1038/s41598-018-34694-z Horna G López M Guerra H Saénz Y Ruiz J. Interplay between MexAB-OprM and MexEF-OprN in clinical isolates of Pseudomonas aeruginosa . Sci Rep . 2018 Nov ; 8 ( 1 ): 16463 . https://doi.org/10.1038/s41598-018-34694-z Search in Google Scholar

Ji J, Wang J, Zhou Z, Wang H, Jiang Y, Yu Y. Multilocus sequence typing reveals genetic diversity of carbapenem- or ceftazidime-non-susceptible Pseudomonas aeruginosa in China. Antimicrob Agents Chemother. 2013;57(11):5697–5700. https://doi.org/10.1128/aac.00970-13 Ji J Wang J Zhou Z Wang H Jiang Y Yu Y. Multilocus sequence typing reveals genetic diversity of carbapenem-or ceftazidime-non-susceptible Pseudomonas aeruginosa in China . Antimicrob Agents Chemother . 2013 ; 57 ( 11 ): 5697 – 5700 . https://doi.org/10.1128/aac.00970-13 Search in Google Scholar

Jolley KA, Bray JE, Maiden MCJ. Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications. Wellcome Open Res. 2018 Sep;3:124. https://doi.org/10.12688/wellcomeopenres.14826.1 Jolley KA Bray JE Maiden MCJ. Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications . Wellcome Open Res . 2018 Sep ; 3 : 124 . https://doi.org/10.12688/wellcomeopenres.14826.1 Search in Google Scholar

Kadri SS, Adjemian J, Lai YL, Spaulding AB, Ricotta E, Prevots DR, Palmore TN, Rhee C, Klompas M, Dekker JP, et al. Difficult-to-treat resistance in gram-negative bacteremia at 173 US hospitals: Retrospective cohort analysis of prevalence, predictors, and outcome of resistance to all first-line agents. Clin Infect Dis. 2018 Nov;67(12):1803–1814. https://doi.org/10.1093/cid/ciy378 Kadri SS Adjemian J Lai YL Spaulding AB Ricotta E Prevots DR Palmore TN Rhee C Klompas M Dekker JP Difficult-to-treat resistance in gram-negative bacteremia at 173 US hospitals: Retrospective cohort analysis of prevalence, predictors, and outcome of resistance to all first-line agents . Clin Infect Dis . 2018 Nov ; 67 ( 12 ): 1803 – 1814 . https://doi.org/10.1093/cid/ciy378 Search in Google Scholar

Karampatakis T, Zarras C, Pappa S, Vagdatli E, Iosifidis E, Roilides E, Papa A. Emergence of ST39 carbapenem-resistant Klebsiella pneumoniae producing VIM-1 and KPC-2. Microb Pathog. 2022 Jan;162:105373. https://doi.org/10.1016/j.micpath.2021.105373 Karampatakis T Zarras C Pappa S Vagdatli E Iosifidis E Roilides E Papa A. Emergence of ST39 carbapenem-resistant Klebsiella pneumoniae producing VIM-1 and KPC-2 . Microb Pathog . 2022 Jan ; 162 : 105373 . https://doi.org/10.1016/j.micpath.2021.105373 Search in Google Scholar

Ladunga I. Installing, maintaining, and using a local copy of BLAST for compute cluster or workstation use. Curr Protoc Bioinformatics. 2018;63(1):e54. https://doi.org/10.1002/cpbi.54 Ladunga I. Installing, maintaining, and using a local copy of BLAST for compute cluster or workstation use . Curr Protoc Bioinformatics . 2018 ; 63 ( 1 ): e54 . https://doi.org/10.1002/cpbi.54 Search in Google Scholar

Li J, Tang M, Liu Z, Wei Y, Xia F, Xia Y, Hu Y, Wang H, Zou M. Molecular characterization of extensively drug-resistant hypervirulent Pseudomonas aeruginosa isolates in China. Ann Clin Microbiol Antimicrob. 2024 Feb;23(1):13. https://doi.org/10.1186/s12941-024-00674-7 Li J Tang M Liu Z Wei Y Xia F Xia Y Hu Y Wang H Zou M. Molecular characterization of extensively drug-resistant hypervirulent Pseudomonas aeruginosa isolates in China . Ann Clin Microbiol Antimicrob . 2024 Feb ; 23 ( 1 ): 13 . https://doi.org/10.1186/s12941-024-00674-7 Search in Google Scholar

Li X, Zhang X, Cai H, Zhu Y, Ji J, Qu T, Tu Y, Zhou H, Yu Y. Over-expression of bla_GES-1 due to a strong promoter in the class 1 integron contributes to decreased ceftazidime-avibactam susceptibility in carbapenem-resistant Pseudomonas aeruginosa ST235. Drug Resist Updat. 2023 Jul;69:100973. https://doi.org/10.1016/j.drup.2023.100973 Li X Zhang X Cai H Zhu Y Ji J Qu T Tu Y Zhou H Yu Y. Over-expression of bla_GES-1 due to a strong promoter in the class 1 integron contributes to decreased ceftazidime-avibactam susceptibility in carbapenem-resistant Pseudomonas aeruginosa ST235 . Drug Resist Updat . 2023 Jul ; 69 : 100973 . https://doi.org/10.1016/j.drup.2023.100973 Search in Google Scholar

Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev. 2009;22(4):582–610. https://doi.org/10.1128/cmr.00040-09 Lister PD Wolter DJ Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms . Clin Microbiol Rev . 2009 ; 22 ( 4 ): 582 – 610 . https://doi.org/10.1128/cmr.00040-09 Search in Google Scholar

Liu Y, Zhu R, Liu X, Li D, Guo M, Fei B, Ren Y, You X, Li Y. Effect of piperine on the inhibitory potential of MexAB-OprM efflux pump and imipenem resistance in carbapenem-resistant Pseudomonas aeruginosa. Microb Pathog. 2023 Dec;185:106397. https://doi.org/10.1016/j.micpath.2023.106397 Liu Y Zhu R Liu X Li D Guo M Fei B Ren Y You X Li Y. Effect of piperine on the inhibitory potential of MexAB-OprM efflux pump and imipenem resistance in carbapenem-resistant Pseudomonas aeruginosa . Microb Pathog . 2023 Dec ; 185 : 106397 . https://doi.org/10.1016/j.micpath.2023.106397 Search in Google Scholar

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔC_T method. Methods. 2001 Dec;25(4):402–408. https://doi.org/10.1006/meth.2001.1262 Livak KJ Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔC _T method . Methods . 2001 Dec ; 25 ( 4 ): 402 – 408 . https://doi.org/10.1006/meth.2001.1262 Search in Google Scholar

Lorusso AB, Carrara JA, Barroso CDN, Tuon FF, Faoro H. Role of efflux pumps on antimicrobial resistance in Pseudomonas aeruginosa. Int J Mol Sci. 2022;23(24):15779. https://doi.org/10.3390/ijms232415779 Lorusso AB Carrara JA Barroso CDN Tuon FF Faoro H. Role of efflux pumps on antimicrobial resistance in Pseudomonas aeruginosa . Int J Mol Sci . 2022 ; 23 ( 24 ): 15779 . https://doi.org/10.3390/ijms232415779 Search in Google Scholar

Ma W, Guo J, Deng C, Huang X, Sun Y, Xu L, Qin Q. Characterization of the chromosomally located metallo-β-Lactamase genes bla_IMP-45 and bla_VIM-2 in a carbapenem-resistant Pseudomonas aeruginosa clinical isolate. Microb Drug Resist. 2024 Oct;30(10):422–431. https://doi.org/10.1089/mdr.2024.0059 Ma W Guo J Deng C Huang X Sun Y Xu L Qin Q. Characterization of the chromosomally located metallo-β-Lactamase genes bla _IMP-45 and bla _VIM-2 in a carbapenem-resistant Pseudomonas aeruginosa clinical isolate . Microb Drug Resist . 2024 Oct ; 30 ( 10 ): 422 – 431 . https://doi.org/10.1089/mdr.2024.0059 Search in Google Scholar

Neves CS, Moura LCRV, Da Costa Lima JL, Maciel MAV. Clinical outcomes of intensive care unit patients infected with multidrug-resistant gram-negative bacteria treated with ceftazidime/avibactam and ceftolozane/tazobactam. Braz J Microbiol. 2024 Mar;55(1):333–341. https://doi.org/10.1007/s42770-023-01193-x Neves CS Moura LCRV Da Costa Lima JL Maciel MAV. Clinical outcomes of intensive care unit patients infected with multidrug-resistant gram-negative bacteria treated with ceftazidime/avibactam and ceftolozane/tazobactam . Braz J Microbiol . 2024 Mar ; 55 ( 1 ): 333 – 341 . https://doi.org/10.1007/s42770-023-01193-x Search in Google Scholar

Ocampo-Sosa AA, Cabot G, Rodríguez C, Roman E, Tubau F, Macia MD, Moya B, Zamorano L, Suárez C, Peña C, et al. Alterations of OprD in carbapenem-intermediate and -susceptible strains of Pseudomonas aeruginosa isolated from patients with bacteremia in a Spanish multicenter study. Antimicrob Agents Chemother. 2012 Apr;56(4):1703–1713.https://doi.org/10.1128/aac.05451-11 Ocampo-Sosa AA Cabot G Rodríguez C Roman E Tubau F Macia MD Moya B Zamorano L Suárez C Peña C Alterations of OprD in carbapenem-intermediate and -susceptible strains of Pseudomonas aeruginosa isolated from patients with bacteremia in a Spanish multicenter study . Antimicrob Agents Chemother . 2012 Apr ; 56 ( 4 ): 1703 – 1713 . https://doi.org/10.1128/aac.05451-11 Search in Google Scholar

Oliver A, Rojo-Molinero E, Arca-Suarez J, Beşli Y, Bogaerts P, Cantón R, Cimen C, Croughs PD, Denis O, Giske CG, et al. Pseudomon asaeruginosa antimicrobial susceptibility profiles, resistance mechanisms and international clonal lineages: Update from ESGARS-ESCMID/ISARPAE Group. Clin Microbiol Infect. 2024;30(4):469–480. https://doi.org/10.1016/j.cmi.2023.12.026 Oliver A Rojo-Molinero E Arca-Suarez J Beşli Y Bogaerts P Cantón R Cimen C Croughs PD Denis O Giske CG Pseudomon asaeruginosa antimicrobial susceptibility profiles, resistance mechanisms and international clonal lineages: Update from ESGARS-ESCMID/ISARPAE Group . Clin Microbiol Infect . 2024 ; 30 ( 4 ): 469 – 480 . https://doi.org/10.1016/j.cmi.2023.12.026 Search in Google Scholar

Pan Y, Zhao M, Liu W, Jia W, Li G. Study on molecular epidemiology of carbapenem resistant Pseudomonas aeruginosa and related genes of quorum sensing signal system. Microb Pathog. 2024 Nov;196:106899. https://doi.org/10.1016/j.micpath.2024.106899 Pan Y Zhao M Liu W Jia W Li G. Study on molecular epidemiology of carbapenem resistant Pseudomonas aeruginosa and related genes of quorum sensing signal system . Microb Pathog . 2024 Nov ; 196 : 106899 . https://doi.org/10.1016/j.micpath.2024.106899 Search in Google Scholar

Porto A, Ayala J, Gutkind G, Di Conza J. A novel OXA-10-like beta-lactamase is present in different Enterobacteriaceae. Diagn Microbiol Infect Dis. 2010;66(2):228–229. https://doi.org/10.1016/j.diagmicrobio.2009.09.010 Porto A Ayala J Gutkind G Di Conza J. A novel OXA-10-like beta-lactamase is present in different Enterobacteriaceae . Diagn Microbiol Infect Dis . 2010 ; 66 ( 2 ): 228 – 229 . https://doi.org/10.1016/j.diagmicrobio.2009.09.010 Search in Google Scholar

Protonotariou E, Meletis G, Vlachodimou N, Malousi A, Tychala A, Katsanou C, Daviti A, Mantzana P, Skoura L. Rapid reversal of carbapenemase-producing Pseudomonas aeruginosa epidemiology from bla_VIM-to bla_NDM-harbouring isolates in a Greek tertiary care hospital. Antibiotics. 2024;13(8):762. https://doi.org/10.3390/antibi-otics13080762 Protonotariou E Meletis G Vlachodimou N Malousi A Tychala A Katsanou C Daviti A Mantzana P Skoura L. Rapid reversal of carbapenemase-producing Pseudomonas aeruginosa epidemiology from bla _VIM-to bla _NDM-harbouring isolates in a Greek tertiary care hospital . Antibiotics . 2024 ; 13 ( 8 ): 762 . https://doi.org/10.3390/antibi-otics13080762 Search in Google Scholar

Queenan AM, Bush K. Carbapenemases: ‘The versatile beta-lactamases. Clin Microbiol Rev. 2007;20(3):440–458. https://doi.org/10.1128/cmr.00001-07 Queenan AM Bush K. Carbapenemases: ‘I he versatile beta-lactamases . Clin Microbiol Rev . 2007 ; 20 ( 3 ): 440 – 458 . https://doi.org/10.1128/cmr.00001-07 Search in Google Scholar

Rossi Gonçalves I, Dantas RCC, Ferreira ML, Batistão DWDF, Gontijo-Filho PP, Ribas RM. Carbapenem-resistant Pseudomonas aeruginosa: Association with virulence genes and biofilm formation. Braz J Microbiol. 2017;48(2):211–217. https://doi.org/10.1016/j.bjm.2016.11.004 Rossi Gonçalves I Dantas RCC Ferreira ML Batistão DWDF Gontijo-Filho PP Ribas RM. Carbapenem-resistant Pseudomonas aeruginosa: Association with virulence genes and biofilm formation . Braz J Microbiol . 2017 ; 48 ( 2 ): 211 – 217 . https://doi.org/10.1016/j.bjm.2016.11.004 Search in Google Scholar

Roy Chowdhury P, Scott M, Worden P, Huntington P, Hudson B, Karagiannis T, Charles IG, Djordjevic SP. Genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant ST235 isolates of Pseudomonas aeruginosa. Open Biol. 2016 Mar;6(3):150175. https://doi.org/10.1098/rsob.150175 Roy Chowdhury P Scott M Worden P Huntington P Hudson B Karagiannis T Charles IG Djordjevic SP. Genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant ST235 isolates of Pseudomonas aeruginosa . Open Biol . 2016 Mar ; 6 ( 3 ): 150175 . https://doi.org/10.1098/rsob.150175 Search in Google Scholar

Santajit S, Indrawattana N. Mechanisms of antimicrobial resistance in ESKAPE pathogens. Biomed Res Int. 2016;2016:2475067. https://doi.org/10.1155/2016/2475067 Santajit S Indrawattana N. Mechanisms of antimicrobial resistance in ESKAPE pathogens . Biomed Res Int . 2016;2016 : 2475067 . https://doi.org/10.1155/2016/2475067 Search in Google Scholar

Sendra E, Fernández-Muñoz A, Zamorano L, Oliver A, Horcajada JP, Juan C, Gómez-Zorrilla S. Impact of multidrug resistance on the virulence and fitness of Pseudomonas aeruginosa: A microbiological and clinical perspective. Infection. 2024 Aug;52(4):1235–1268. https://doi.org/10.1007/s15010-024-02313-x Sendra E Fernández-Muñoz A Zamorano L Oliver A Horcajada JP Juan C Gómez-Zorrilla S. Impact of multidrug resistance on the virulence and fitness of Pseudomonas aeruginosa: A microbiological and clinical perspective . Infection . 2024 Aug ; 52 ( 4 ): 1235 – 1268 . https://doi.org/10.1007/s15010-024-02313-x Search in Google Scholar

Seupt A, Schniederjans M, Tomasch J, Häussler S. Expression of the MexXY aminoglycoside efflux pump and presence of an aminoglycoside-modifying enzyme in clinical Pseudomonas aeruginosa isolates are highly correlated. Antimicrob Agents Chemother. 2020;65(1):e01166–20. https://doi.org/10.1128/aac.01166-20 Seupt A Schniederjans M Tomasch J Häussler S . Expression of the MexXY aminoglycoside efflux pump and presence of an aminoglycoside-modifying enzyme in clinical Pseudomonas aeruginosa isolates are highly correlated . Antimicrob Agents Chemother . 2020 ; 65 ( 1 ): e01166 – 20 . https://doi.org/10.1128/aac.01166-20 Search in Google Scholar

Sherrard LJ, Wee BA, Duplancic C, Ramsay KA, Dave KA, Ballard E, Wainwright CE, Grimwood K, Sidjabat HE, Whiley DM, et al. Emergence and impact of oprD mutations in Pseudomonas aeruginosa strains in cystic fibrosis. J Cyst Fibros. 2022 Jan;21(1):e35–e43. https://doi.org/10.1016/j.jcf.2021.03.007 Sherrard LJ Wee BA Duplancic C Ramsay KA Dave KA Ballard E Wainwright CE Grimwood K Sidjabat HE Whiley DM Emergence and impact of oprD mutations in Pseudomonas aeruginosa strains in cystic fibrosis . J Cyst Fibros . 2022 Jan ; 21 ( 1 ): e35 – e43 . https://doi.org/10.1016/j.jcf.2021.03.007 Search in Google Scholar

Silveira MC, Albano RM, Rocha-de-Souza CM, Leão RS, Marques EA, Picão RC, Kraychete GB, de Oliveira Santos IC, Oliveira TRTE, Tavares-Teixeira CB, et al. Description of a novel IncP plasmid harboring bla_KPC-2 recovered from a SPM-1-producing Pseudomonas aeruginosa from ST277. Infect Genet Evol. 2022 Aug;102:105302. https://doi.org/10.1016/j.meegid.2022.105302 Silveira MC Albano RM Rocha-de-Souza CM Leão RS Marques EA Picão RC Kraychete GB de Oliveira Santos IC Oliveira TRTE Tavares-Teixeira CB Description of a novel IncP plasmid harboring bla _KPC-2 recovered from a SPM-1-producing Pseudomonas aeruginosa from ST277 . Infect Genet Evol . 2022 Aug ; 102 : 105302 . https://doi.org/10.1016/j.meegid.2022.105302 Search in Google Scholar

Sun Z, Yang F, Ji J, Cao W, Liu C, Ding B, Xu X. Dissecting the genotypic features of a fluoroquinolone-resistant Pseudomonas aeruginosa ST316 sublineage causing ear infections in Shanghai, China. Microb Genom. 2023;9(4):mgen000989. https://doi.org/10.1099/mgen.0.000989 Sun Z Yang F Ji J Cao W Liu C Ding B Xu X. Dissecting the genotypic features of a fluoroquinolone-resistant Pseudomonas aeruginosa ST316 sublineage causing ear infections in Shanghai, China . Microb Genom . 2023 ; 9 ( 4 ): mgen000989 . https://doi.org/10.1099/mgen.0.000989 Search in Google Scholar

Tenover FC, Nicolau DP, Gill CM. Carbapenemase-producing Pseudomonas aeruginosa -an emerging challenge. Emerg Microbes Infect. 2022 Dec;11(1):811–814. https://doi.org/10.1080/22221751.2022.2048972 Tenover FC Nicolau DP Gill CM. Carbapenemase-producing Pseudomonas aeruginosa -an emerging challenge . Emerg Microbes Infect . 2022 Dec ; 11 ( 1 ): 811 – 814 . https://doi.org/10.1080/22221751.2022.2048972 Search in Google Scholar

Tomás M, Doumith M, Warner M, Turton JF, Beceiro A, Bou G, Livermore DM, Woodford N. Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients. Antimicrob Agents Chemother. 2010 May;54(5):2219–2224. https://doi.org/10.1128/aac.00816-09 Tomás M Doumith M Warner M Turton JF Beceiro A Bou G Livermore DM Woodford N. Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients . Antimicrob Agents Chemother . 2010 May ; 54 ( 5 ): 2219 – 2224 . https://doi.org/10.1128/aac.00816-09 Search in Google Scholar

Tran HA, Vu TNB, Trinh ST, Tran DL, Pham HM, Ngo THH, Nguyen MT, Tran ND, Pham DT, Dang DA, et al. Resistance mechanisms and genetic relatedness among carbapenem-resistant Pseudomonas aeruginosa isolates from three major hospitals in Hanoi, Vietnam (2011–15). JAC Antimicrob Resist. 2021 Jul;3(3):dlab103. https://doi.org/10.1093/jacamr/dlab103 Tran HA Vu TNB Trinh ST Tran DL Pham HM Ngo THH Nguyen MT Tran ND Pham DT Dang DA Resistance mechanisms and genetic relatedness among carbapenem-resistant Pseudomonas aeruginosa isolates from three major hospitals in Hanoi, Vietnam (2011–15) . JAC Antimicrob Resist . 2021 Jul ; 3 ( 3 ): dlab103 . https://doi.org/10.1093/jacamr/dlab103 Search in Google Scholar

Verdial C, Serrano I, Tavares L, Gil S, Oliveira M. Mechanisms of antibiotic and biocide resistance that contribute to Pseudomonas aeruginosa persistence in the hospital environment. Biomedicines. 2023 Apr;11(4):1221. https://doi.org/10.3390/biomedi-cines11041221 Verdial C Serrano I Tavares L Gil S Oliveira M. Mechanisms of antibiotic and biocide resistance that contribute to Pseudomonas aeruginosa persistence in the hospital environment . Biomedicines . 2023 Apr ; 11 ( 4 ): 1221 . https://doi.org/10.3390/biomedi-cines11041221 Search in Google Scholar

Wang L, Zhou X, Lu Y, Zhang X, Jiang J, Sun Z, Yin M, Doi Y, Wang M, Guo Q, et al. Levofloxacin-induced MexS mutation triggers imipenem-relebactam resistance in a KPC-producing Pseudomonas aeruginosa. Int J Antimicrob Agents. 2024;63(5):107119. https://doi.org/10.1016/j.ijantimicag.2024.107119 Wang L Zhou X Lu Y Zhang X Jiang J Sun Z Yin M Doi Y Wang M Guo Q Levofloxacin-induced MexS mutation triggers imipenem-relebactam resistance in a KPC-producing Pseudomonas aeruginosa . Int J Antimicrob Agents . 2024 ; 63 ( 5 ): 107119 . https://doi.org/10.1016/j.ijantimicag.2024.107119 Search in Google Scholar

Wang X, Kong N, Cao M, Zhang L, Sun M, Xiao L, Li G, Wei Q. Comparison of class 2 integron integrase activities. Curr Microbiol. 2021 Mar;78(3):967–978. https://doi.org/10.1007/s00284-021-02352-9 Wang X Kong N Cao M Zhang L Sun M Xiao L Li G Wei Q. Comparison of class 2 integron integrase activities . Curr Microbiol . 2021 Mar ; 78 ( 3 ): 967 – 978 . https://doi.org/10.1007/s00284-021-02352-9 Search in Google Scholar

Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017 Jun;13(6):e1005595. https://doi.org/10.1371/journal.pcbi.1005595 Wick RR Judd LM Gorrie CL Holt KE. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads . PLoS Comput Biol . 2017 Jun ; 13 ( 6 ): e1005595 . https://doi.org/10.1371/journal.pcbi.1005595 Search in Google Scholar

Wick RR, Schultz MB, Zobel J, Holt KE. Bandage: Interactive visualization of de novo genome assemblies. Bioinformatics. 2015;31(20):3350–3352. https://doi.org/10.1093/bioinformatics/btv383 Wick RR Schultz MB Zobel J Holt KE. Bandage: Interactive visualization of de novo genome assemblies . Bioinformatics . 2015 ; 31 ( 20 ): 3350 – 3352 . https://doi.org/10.1093/bioinformatics/btv383 Search in Google Scholar

Xiu Y, Dai Y, Yin S, Wei Q. Analysis of the class 1 integrons, carbapenemase genes and biofilm formation genes occurrence in Acinetobacter baumannii clinical Isolates. Pol J Microbiol. 2024 May;73(2):189–197. https://doi.org/10.33073/pjm-2024-017 Xiu Y Dai Y Yin S Wei Q . Analysis of the class 1 integrons, carbapenemase genes and biofilm formation genes occurrence in Acinetobacter baumannii clinical Isolates . Pol J Microbiol . 2024 May ; 73 ( 2 ): 189 – 197 . https://doi.org/10.33073/pjm-2024-017 Search in Google Scholar

Zahedi Bialvaei A, Rahbar M, Hamidi-Farahani R, Asgari A, Esmailkhani A, Mardani Dashti Y, Soleiman-Meigooni S. Expression of RND efflux pumps mediated antibiotic resistance in Pseudomonas aeruginosa clinical strains. Microb Pathog. 2021;153:104789. https://doi.org/10.1016/j.micpath.2021.104789 Zahedi Bialvaei A Rahbar M Hamidi-Farahani R Asgari A Esmailkhani A Mardani Dashti Y Soleiman-Meigooni S. Expression of RND efflux pumps mediated antibiotic resistance in Pseudomonas aeruginosa clinical strains . Microb Pathog . 2021 ; 153 : 104789 . https://doi.org/10.1016/j.micpath.2021.104789 Search in Google Scholar

Zhang P, Hu J, Wu W, Shi W, Jiang Y, Yu Y, Zheng X, Qu T. Evolutionary adaptation of KPC-2-producing Pseudomonas aeruginosa high-risk sequence type 463 in a lung transplant patient. Int J Antimicrob Agents. 2024 Sep;64(3):107279. https://doi.org/10.1016/j.ijantimicag.2024.107279 Zhang P Hu J Wu W Shi W Jiang Y Yu Y Zheng X Qu T. Evolutionary adaptation of KPC-2-producing Pseudomonas aeruginosa high-risk sequence type 463 in a lung transplant patient . Int J Antimicrob Agents . 2024 Sep ; 64 ( 3 ): 107279 . https://doi.org/10.1016/j.ijantimicag.2024.107279 Search in Google Scholar

Zhang X, Wang L, Li D, Li P, Yuan L, Yang F, Guo Q, Wang M. An IncP-2 plasmid sublineage associated with dissemination of bla_IMP-45 among carbapenem-resistant Pseudomonas aeruginosa. Emerg Microbes Infect. 2021;10(1):442–449. https://doi.org/10.1080/22221751.2021.1894903 Zhang X Wang L Li D Li P Yuan L Yang F Guo Q Wang M. An IncP-2 plasmid sublineage associated with dissemination of bla _IMP-45 among carbapenem-resistant Pseudomonas aeruginosa . Emerg Microbes Infect . 2021 ; 10 ( 1 ): 442 – 449 . https://doi.org/10.1080/22221751.2021.1894903 Search in Google Scholar

Zhao X, Qin J, Chen G, Yang C, Wei J, Li W, Jia W. Whole-genome sequencing, multilocus sequence typing, and resistance mechanism of the carbapenem-resistant Pseudomonas aeruginosa in China. Microb Pathog. 2024 Jul;192:106720. https://doi.org/10.1016/j.mic-path.2024.106720 Zhao X Qin J Chen G Yang C Wei J Li W Jia W. Whole-genome sequencing, multilocus sequence typing, and resistance mechanism of the carbapenem-resistant Pseudomonas aeruginosa in China . Microb Pathog . 2024 Jul ; 192 : 106720 . https://doi.org/10.1016/j.mic-path.2024.106720 Search in Google Scholar

Zhu Y, Wang T, Zhu W, Wei Q. Influence of class 2 integron integrase concentration on gene cassette insertion and excision in vivo. Braz J Microbiol. 2023 Jun;54(2):645–653. https://doi.org/10.1007/s42770-023-00926-2 Zhu Y Wang T Zhu W Wei Q. Influence of class 2 integron integrase concentration on gene cassette insertion and excision in vivo . Braz J Microbiol . 2023 Jun ; 54 ( 2 ): 645 – 653 . https://doi.org/10.1007/s42770-023-00926-2 Search in Google Scholar