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Analysis of the Class 1 Integrons, Carbapenemase Genes and Biofilm Formation Genes Occurrence in Acinetobacter baumannii Clinical Isolates

Yu Xiu
Yu Xiu
, 
Yueru Dai
Yueru Dai
, 
Shasha Yin
Shasha Yin
 oraz 
Quhao Wei
Quhao Wei
   | 29 maj 2024
Polish Journal of Microbiology's Cover Image
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Article Category: Original Paper
Data publikacji: 29 maj 2024
Zakres stron: 189 - 197
Otrzymano: 09 gru 2023
Przyjęty: 28 mar 2024
DOI: https://doi.org/10.33073/pjm-2024-017
Słowa kluczowe
© 2024 Yu Xiu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Clinical ward distribution of CRAB and CSAB isolates.
Clinical ward distribution of CRAB and CSAB isolates.

Fig. 2.

Clinical specimen type distribution of CRAB and CSAB isolates.
Clinical specimen type distribution of CRAB and CSAB isolates.

Primers used in this study.

Primer Primer sequence (5’–3’) Target gene References
intF CCAAGCTCTCGGGTAACATC intI1 Wang et al. 2023
P2R CCCGAGGCATAGACTGTA
5CS GGCATCCAAGCAGCAAG variable region Wang et al. 2023
3CS AAGCAGACTTGACCTGA
AACF ATCTCATATCGTCGAGTGG aac(6’)-Ib Wang et al. 2023
AACR TGCGTGTTCGCTCGAATGC
AADAF GCAGCGCAATGACATTCTTG aadA1 Wang et al. 2023
AADAR ATCCTTCGGCGCGATTTTG
CATF TTACTCTGGCTACTATCAC catB8 Wang et al. 2023
CATR TGATGGCATAAGGCTCTAC
KPC-F CGTCTAGTTCTGCTGTCTTG blaKPC Wang et al. 2023
KPC-R CTTGTCATCCTTGTTAGGCG
VIM-F GATGGTGTTTGGTCGCATA blaVIM Wang et al.2023
VIM-R CGAATGCGCAGCACCAG
NDM-F GGTTTGGCGATCTGGTTTTC blaNDM Wang et al. 2023
NDM-R CGGAATGGCTCATCACGATC
OXA-23-like-F CCGTCGTTTACGACATTCA blaOXA-23-like Wang et al.2023
OXA-23-like-R AAAGAGCGCATTGCTTTGAT
IMP-F GGAATAGAGTGGCTTAAYTCTC blaIMP Wang et al.2023
IMP-R GGTTTAAYAAAACAACCACC
bfmS-F ATATATGCGGGGCTGGTAATTC bfms Cai et al. 2017
bfmS-R ATGCAGGTGCTTTTTTATTGGT
cusE-F ATGCATGTTCTCTGGACTGATGTTGAC cusE Cai et al. 2017
cusE-R CGACTTGTACCGTGACCGTATCTTGATAAG
bap-F CGTTTCCTGGGTCTGATGTATT bap Cai et al. 2017
bap-R GTTATTGAAGGCTTCTTTAGTG
abal-F GTGGCTCAAGACAGAGAATC abal Wang and Ye 2021
abal-R TCAATCATCATTGGTGGACC This study

Virulence- and resistance-gene-carrying status of CRAB and CSAB.

Genes CRAB (n = 219) CSAB (n = 50) p
abal + 204 19 0*
– 15 31
bfmS + 140 31 0.798
– 79 19
bap + 193 5 0*
– 26 45
cusE + 170 24 0*
– 49 26
blaOXA-23-like + 218 0 0*
– 1 50

Resistance rates of CRAB and CSAB isolates to commonly used antibiotics.

Antibiotics CRAB (n = 219) CSAB (n = 50) p
No. Rate (%) No. Rate (%)
CAZ 218 99.54 6 12.00 0.000*
CIP 217 99.09 6 12.00 0.000*
TOB 206 94.06 6 12.00 0.000*
DOX 216 98.63 6 12.00 0.000*
SXT 166 75.80 8 16.00 0.000*
LVX 175 79.91 5 10.00 0.000*
AMK 131 59.82 6 12.00 1.000
FEP 169 77.17 6 12.00 0.384
MNO 78 35.62 2 4.00 0.000*
TGC 11 5.02 0 0.00 0.226
COL 3 1.37 0 0.00 1.000

Correlation between class 1 integron and antibiotic resistant rates in 219 CRAB isolates.

Antibiotics intI1(+) (n = 75) intI1(−) (n = 144) p
No. Rate (%) No. Rate (%)
CAZ 75 100 143 99.31 1.000
CIP 75 100 142 98.61 0.548
TOB 75 100 132 91.67 0.009*
DOX 74 98.67 142 98.61 1.000
SXT 73 97.33 93 64.58 0.000*
LVX 62 82.67 113 78.47 0.462
AMK 58 77.33 78 54.17 0.001*
FEP 44 58.67 125 86.81 0.000*
MNO 36 48 42 29.17 0.006*
TGC 1 1.33 10 6.94 0.103
COL 1 1.33 2 1.39 1.000

Correlation between class 1 integron and virulence and resistance genes in 219 CRAB isolates.

Genes intI1(+) (n = 75) intI1(–) (n = 144) p
abal + 75 129 0.003*
– 0 15
bfmS + 53 87 0.134
– 22 57
bap + 72 121 0.009*
– 3 23
cusE + 53 116 0.088
– 22 28
blaOXA-23-like + 75 143 1.000
– 0 1

Correlations between virulence genes and antibiotic resistance in 219 CRAB isolates.

Antibiotics abal bfmS cusE bap
+ (n = 204) – (n = 15) p + (n = 140) – (n = 79) p + (n = 170) – (n = 49) p + (n = 193) – (n = 26) p
MNO 72 6 0.782 47 31 0.4 59 19 0.6 68 10 0.747
DOX 201 15 1 139 77 0.296 168 48 0.534 191 25 0.317
SXT 154 12 1 98 68 0.008* 124 42 0.066 145 21 0.528
CIP 202 15 1 139 78 1 168 49 1 192 25 0.224
LVX 163 12 1 109 66 0.313 141 34 0.037* 152 23 0.246
TOB 191 15 0.607 129 77 0.142 161 45 0.494 185 21 0.011*
AMK 125 6 0.105 79 52 0.173 96 35 0.06 119 12 0.13
TGC 11 0 1 9 2 0.335 9 2 1 11 0 0.369
CAZ 203 15 1 139 79 1 169 49 1 192 26 1
FEP 156 13 0.74 112 57 0.184 141 28 0* 148 21 0.641
COL 3 0 1 2 1 1 2 1 0.534 2 1 0.317

Results of multilocus sequence typing (MLST).

Oxford ST gltA gyrB gdhB recA cpn60 gpi rpoD
3272 1 12 56 1 1 385 26
Pasteur ST cpn60 fusA gltA pyrG recA rplB rpoB
2520 3 2 2 2 3 4 95
eISSN:
2544-4646
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Microbiology and Virology
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