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A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae

Siyu Zeng

Siyu Zeng

Department of Pharmacy and Laboratory, Huizhou Health Sciences PolytechnicHuizhou, China
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Zeng, Siyu
, 
Yinghua Lu

Yinghua Lu

College of Chemistry and Chemical Engineering, Xiamen UniversityXiamen, China
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Lu, Yinghua
, 
Xueshan Pan

Xueshan Pan

College of Chemistry and Chemical Engineering, Xiamen UniversityXiamen, China
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Pan, Xueshan
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Xueping Ling

Xueping Ling

College of Chemistry and Chemical Engineering, Xiamen UniversityXiamen, China
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Ling, Xueping
  
24 wrz 2022
A Novel Bioflocculant Produced by Cobetia marina MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae's Cover Image
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Kategoria artykułu: Original Paper
Data publikacji: 24 wrz 2022
Zakres stron: 341 - 351
Otrzymano: 14 mar 2022
Przyjęty: 03 lip 2022
DOI: https://doi.org/10.33073/pjm-2022-030
Słowa kluczowe
© 2022 Siyu Zeng et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Fig. 1

Temporal courses of the a) bioflocculant, b) yield of biomass, and c) consumption of glucose, under different initial levels of glucose.
Temporal courses of the a) bioflocculant, b) yield of biomass, and c) consumption of glucose, under different initial levels of glucose.

Fig. 2

a) Effects of the N source alone on the biomass and bioflocculant production, b) effect of the ratio of combined N source on the biomass and bioflocculant production. A/YE – yeast extract, B/Glu – glutamate, C – tryptone, D – ammonium nitrate, E – ammonia chloride, F – urea. The control group was cultured in a minimal medium.
a) Effects of the N source alone on the biomass and bioflocculant production, b) effect of the ratio of combined N source on the biomass and bioflocculant production. A/YE – yeast extract, B/Glu – glutamate, C – tryptone, D – ammonium nitrate, E – ammonia chloride, F – urea. The control group was cultured in a minimal medium.

Fig. 3

The change curve of pH for the a) biomass and b) bioflocculant.
The change curve of pH for the a) biomass and b) bioflocculant.

Fig. 4

RSM and contour plots illustrating the interplaying effects of various factors on bioflocculant production; a–b) glucose (g/l) and yeast extract (g/l), c–d) glucose (g/l) and glutamate (g/l), e–f) yeast extract (g/l) and glutamate (g/l).
RSM and contour plots illustrating the interplaying effects of various factors on bioflocculant production; a–b) glucose (g/l) and yeast extract (g/l), c–d) glucose (g/l) and glutamate (g/l), e–f) yeast extract (g/l) and glutamate (g/l).

Fig. 5

Temporal courses of cellular growth, bioflocculant production, and pH for C. marina MCCC1113 cultivated in the optimal medium inside a rotary shaker at 150 rpm and 28°C for 72 h.
Temporal courses of cellular growth, bioflocculant production, and pH for C. marina MCCC1113 cultivated in the optimal medium inside a rotary shaker at 150 rpm and 28°C for 72 h.

Flocculation efficiency of bioflocculant produced by C_ marina on various microalgae_

Flocculation time (min) Group H. pluvialis C. vulgaris S. platensis
FE (%)
5 Treatment 32.1 – 69.4
Control 26.4 – 32.9

Effect analysis of independent variables in Plackett-Burman design_

Factor Level p Significant
–1 1
Glucose 25 35 0.0022 ++
Yeast extract 5 15 0.004 ++
Glutamate 1 3 0.025 +
Culture time 28 36 0.85 –
pH 7 8 0.861 –

ANOVA for response surface quadratic model_

Source Sum of square DF Mean square F-value Prob > F
Model 0.76   9 0.084 7.02 0.0088
Residual   0.084   7 0.012
Lack of fit   0.039   3 0.013 1.16 0.4282
Pure error   0.045   4
Cor total 0.84 16
R2 = 0.9803 R2adj = 0.7721 R2pred = 0.1744 CV = 13.53 Adeq. precision = 7.526

Determination of the optimum ratio of bioflocculant/microalgae (v/v)_

FE (%) of different algae Amount of BF/algae added (%)
0 5 10 15 20
H. pluvialis 20 34.8 36.7 47.2 63
S. platensis 32.9 86.4 87.8 89.4 93.9
C. vulgaris 29.1 48.1 53.2 53.1 51.1

Box-Behnken design arrangement and responses_

RUN Glucose Yeast extract Glutamate Bioflocculant
(g/l) (g/l) (g/l) (g/l)
1 30 10 2 1.30
2 30 10 2 1.19
3 30 5 1 0.9
4 35 5 2 0.65
5 30 15 3 0.95
6 30 10 2 0.89
7 35 10 3 0.79
8 25 15 2 0.57
9 30 5 3 0.87
10 25 10 1 0.32
11 25 5 2 0.54
12 35 10 1 0.79
13 30 10 2 1.22
14 30 10 2 1.28
15 30 15 1 0.92
16 25 10 3 0.58
17 35 15 2 0.65
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Mikrobiologia i wirusologia
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