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The Relation Between Host TLR9 -1486T/C, rs187084 Gene Polymorphisms and Helicobacter pylori cagA, sodB, hsp60, and vacA Virulence Genes among Gastric Cancer Patients

Amira M. Sultan
Amira M. Sultan
, 
Ragy Shenouda
Ragy Shenouda
, 
Ahmad M. Sultan
Ahmad M. Sultan
, 
Ahmed Shehta
Ahmed Shehta
 oraz 
Yasmin Nabiel
Yasmin Nabiel
   | 27 lut 2022
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Article Category: Original Paper
Data publikacji: 27 lut 2022
Zakres stron: 35 - 42
Otrzymano: 13 paź 2021
Przyjęty: 19 gru 2021
DOI: https://doi.org/10.33073/pjm-2022-003
Słowa kluczowe
© 2022 Amira M. Sultan et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Sequences of primer sets used.

Gene targeted Sequence Size of amplified product (bp) Ref
glmM F: 5′-AAGCTTTTAGGGGTGTTAGGGGTTT-3′R: 5′-AAGCTTACTTTCTAACACTAACGC-3′ 294 Santos et al. 2012
cagA F: 5′-GATAACAGGCAAGCTTTTGAGG-3′R: 5′-CTGCAAAAGATTGTTTGGCAG-3′ 349 Amin et al. 2019
sodB F: 5′-GCCCTGTGGCGTTTGATTTCC-3′R: 5′-CATGCTCCCACACATCCACC-3′ 425 Ryberg et al. 2008
hsp60 F: 5′-GCTCCAAGCATCACCAAAGACG-3′R: 5′-GCGGTTTGCCCTCTTTCATGG-3′ 603 Ryberg et al. 2008
vacA F: 5′-CAATCGTGTGGGTTCTGGAGC-3′R: 5′-GCCGATATGCAAATGAGCCGC-3′ 678 Ryberg et al. 2008
vacAs1/s2 F: 5′-ATGGAAATACAACAAACACAC-3′R: 5′-CTGCTTGAATGCGCCAAAC-3′ 259 Harrison et al. 2017
vacAm1 F: 5′-GGTCAAAATGCGGTCATGG-3′R: 5′-CCATTGGTACCTGTAGAAAC-3′ 290 Harrison et al. 2017
vacAm2 F: 5′-CATAACTAGCGCCTTGCAC-3′R: 5′-GGAGCCCCAGGAAACATTG-3′ 352 Harrison et al. 2017
TLR9-1486T/C, rs187084 5′-TTCATTCATTCAGCCTTCACTCA-3′5′-GAGTCAAAGCCACAGTCCACA-3′ 490 Roszak et al. 2012

Distribution of H. pylori among gastric tissue samples in studied gastric cancer patients.

Number % p-value
Positive 106 80.3 0.000*
Negative 26 19.7
Total 132 100%

Distribution of genotypes of TLR9 -1486T/C, rs187084 polymorphism in studied groups.

Genotype frequency Healthy study participants N = 106 Gastric cancer patients N = 106 OR 95% CI X2 p-value
N % N %
TT 40 37.7 22 20.8 1.18 0.45–3.1 0.12 0.73
TC 42 39.6 28 26.4 1.2 0.49–2.88 0.16 0.68
CC 24 22.6 56 52.8 2.68 1.0–7.14 4.03 0.045*

Demographic and histopathological data of the subjects included in the study.

Variable Gastric cancer patients Healthy study participants p-value
Age
Years (mean ± SD) 56.55 ± 8.63 53.27 ± 8.55 0.93
Sex
Male 63 (59.4) 59 (55.7%) 0.76
Female 43 (40.6%) 47 (44.3%)
Differentiation of the tumor
Well differentiated tumor 16 (15.1%) NA –
Moderately differentiated tumor 35 (33.0%)
Poorly differentiated tumor 55 (51.9%)

Distribution of virulence genes in H. pylori strains isolated from gastric cancer patients.

Virulence gene N = 106 % p-value
cagA
Positive 96 90.6 0.000*
Negative 10 9.4
sodB
Positive 75 70.8 0.000*
Negative 31 29.2
hsp60
Positive 88 83.0 0.000*
Negative 18 17.0
vacA
Positive 101 95.3 0.000*
Negative 5 4.7
vacAs1/s2
Positive 101 95.3 0.000*
Negative 5 4.7
vacAm1
Positive 56 52.8 0.56
Negative 50 47.2
vacAm2
Positive 45 42.5 0.12
Negative 61 57.5

The relation between virulence genes of H. pylori and TLR9 -1486T/C, rs187084 genotypes in gastric cancer patients.

TLR9-1486T/C, rs187084 genotype CC N = 56 TT + TC N = 50 p-value
Virulence gene N % N %
cagA 55 98.2 41 82.0 0.075
sodB 53 94.6 22 44.0 0.021*
hsp60 53 94.6 35 70.0 0.049*
vacA 54 96.4 47 94.0 0.88
vacAs1/s2 54 96.4 47 94.0 0.88
vacAm1 35 62.5 21 42.0 0.048*
vacAm2 19 33.9 26 52.0 0.81
eISSN:
2544-4646
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Microbiology and Virology
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