The Relation Between Host TLR9 -1486T/C, rs187084 Gene Polymorphisms and , , , and  Virulence Genes among Gastric Cancer Patients

Gene targeted	Sequence	Size of amplified product (bp)	Ref
glmM	F: 5′-AAGCTTTTAGGGGTGTTAGGGGTTT-3′R: 5′-AAGCTTACTTTCTAACACTAACGC-3′	294	Santos et al. 2012
cagA	F: 5′-GATAACAGGCAAGCTTTTGAGG-3′R: 5′-CTGCAAAAGATTGTTTGGCAG-3′	349	Amin et al. 2019
sodB	F: 5′-GCCCTGTGGCGTTTGATTTCC-3′R: 5′-CATGCTCCCACACATCCACC-3′	425	Ryberg et al. 2008
hsp60	F: 5′-GCTCCAAGCATCACCAAAGACG-3′R: 5′-GCGGTTTGCCCTCTTTCATGG-3′	603	Ryberg et al. 2008
vacA	F: 5′-CAATCGTGTGGGTTCTGGAGC-3′R: 5′-GCCGATATGCAAATGAGCCGC-3′	678	Ryberg et al. 2008
vacAs1/s2	F: 5′-ATGGAAATACAACAAACACAC-3′R: 5′-CTGCTTGAATGCGCCAAAC-3′	259	Harrison et al. 2017
vacAm1	F: 5′-GGTCAAAATGCGGTCATGG-3′R: 5′-CCATTGGTACCTGTAGAAAC-3′	290	Harrison et al. 2017
vacAm2	F: 5′-CATAACTAGCGCCTTGCAC-3′R: 5′-GGAGCCCCAGGAAACATTG-3′	352	Harrison et al. 2017
TLR9-1486T/C, rs187084	5′-TTCATTCATTCAGCCTTCACTCA-3′5′-GAGTCAAAGCCACAGTCCACA-3′	490	Roszak et al. 2012

Genotype frequency	Healthy study participants N = 106		Gastric cancer patients N = 106		OR	95% CI	X²	p-value
Genotype frequency	N	%	N	%	OR	95% CI	X²	p-value
TT	40	37.7	22	20.8	1.18	0.45–3.1	0.12	0.73
TC	42	39.6	28	26.4	1.2	0.49–2.88	0.16	0.68
CC	24	22.6	56	52.8	2.68	1.0–7.14	4.03	0.045^*

Variable	Gastric cancer patients	Healthy study participants	p-value
Age
Years (mean ± SD)	56.55 ± 8.63	53.27 ± 8.55	0.93
Sex
Male	63 (59.4)	59 (55.7%)	0.76
Female	43 (40.6%)	47 (44.3%)
Differentiation of the tumor
Well differentiated tumor	16 (15.1%)	NA	–
Moderately differentiated tumor	35 (33.0%)
Poorly differentiated tumor	55 (51.9%)

Virulence gene	N = 106	%	p-value
cagA
Positive	96	90.6	0.000^*
Negative	10	9.4	0.000^*
sodB
Positive	75	70.8	0.000^*
Negative	31	29.2
hsp60
Positive	88	83.0	0.000^*
Negative	18	17.0	0.000^*
vacA
Positive	101	95.3	0.000^*
Negative	5	4.7	0.000^*
vacAs1/s2
Positive	101	95.3	0.000^*
Negative	5	4.7	0.000^*
vacAm1
Positive	56	52.8	0.56
Negative	50	47.2	0.56
vacAm2
Positive	45	42.5	0.12
Negative	61	57.5	0.12

TLR9-1486T/C, rs187084 genotype	CC N = 56		TT + TC N = 50		p-value
Virulence gene	N	%	N	%	p-value
cagA	55	98.2	41	82.0	0.075
sodB	53	94.6	22	44.0	0.021^*
hsp60	53	94.6	35	70.0	0.049^*
vacA	54	96.4	47	94.0	0.88
vacAs1/s2	54	96.4	47	94.0	0.88
vacAm1	35	62.5	21	42.0	0.048^*
vacAm2	19	33.9	26	52.0	0.81

The Relation Between Host TLR9 -1486T/C, rs187084 Gene Polymorphisms and Helicobacter pylori cagA, sodB, hsp60, and vacA Virulence Genes among Gastric Cancer Patients