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Effects of morphometric and biochemical parameters on collagen and pepsin-solubilized collagen yields of Holothuria tubulosa (Gmelin, 1790) and Holothuria (Roweothuria) poli (Delle Chiaje, 1823)

İbrahim Ender Künili
İbrahim Ender Künili
   | 31 mar 2022
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Article Category: Original research paper
Data publikacji: 31 mar 2022
Zakres stron: 100 - 114
Otrzymano: 01 kwi 2021
Przyjęty: 24 sie 2021
DOI: https://doi.org/10.26881/oahs.2022.1.09
Słowa kluczowe
© 2022 İbrahim Ender Künili, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Sampling locations: 1 – Kemikli (40°16’39”N; 26°14’10”E); 2 – Dardanos (40°4’26”N; 26°21’16”E); 3 – Yapıldak (40°12’26”N; 26°28’36”E); 4 – Ocaklar (40°12’26”N; 27°44’46”E).
Sampling locations: 1 – Kemikli (40°16’39”N; 26°14’10”E); 2 – Dardanos (40°4’26”N; 26°21’16”E); 3 – Yapıldak (40°12’26”N; 26°28’36”E); 4 – Ocaklar (40°12’26”N; 27°44’46”E).

Figure 2

Relationship between H. tubulosa collagen yield and length and biochemical composition (mean ± SE).
Relationship between H. tubulosa collagen yield and length and biochemical composition (mean ± SE).

Figure 3

Relationship between H. poli collagen yield and length and biochemical composition (mean ± SE).
Relationship between H. poli collagen yield and length and biochemical composition (mean ± SE).

Figure 4

Correlation between collagen yield and major determinants in both sea cucumber species. GW – gutted weight, GSI – gonadosomatic index.
Correlation between collagen yield and major determinants in both sea cucumber species. GW – gutted weight, GSI – gonadosomatic index.

Figure 5

Changes in collagen yield in mature (M) and immature (IM) H. tubulosa individuals compared with morphometric parameters (mean weight ± SE).
Changes in collagen yield in mature (M) and immature (IM) H. tubulosa individuals compared with morphometric parameters (mean weight ± SE).

Figure 6

Changes in collagen yield in mature (M) and immature (IM) H. poli individuals compared with morphometric parameters (mean weight ± SE).
Changes in collagen yield in mature (M) and immature (IM) H. poli individuals compared with morphometric parameters (mean weight ± SE).

Figure 7

a) Hydrolyzation degrees (HD) of collagens in relation to different enzyme concentrations in H. tubulosa and H. poli (mean ± SE); b) Pearson correlations between pepsin-solubilized collagen (PSC), morphometric and biochemical parameters as major determinants. GW – gutted weight; GSI – gonadosomatic index.
a) Hydrolyzation degrees (HD) of collagens in relation to different enzyme concentrations in H. tubulosa and H. poli (mean ± SE); b) Pearson correlations between pepsin-solubilized collagen (PSC), morphometric and biochemical parameters as major determinants. GW – gutted weight; GSI – gonadosomatic index.

Figure 8

Pepsin-solubilized collagen (PSC) yields in H. tubulosa (a) and H. poli (b) in relation to different enzyme concentrations (EC) and length frequencies (mean percentage in wet weight of body wall; % ± SD).
Pepsin-solubilized collagen (PSC) yields in H. tubulosa (a) and H. poli (b) in relation to different enzyme concentrations (EC) and length frequencies (mean percentage in wet weight of body wall; % ± SD).

Figure 9

SDS-PAGE pattern of pepsin-solubilized collagen in H. tubulosa (HT) and H. poli (HP). M – protein marker.
SDS-PAGE pattern of pepsin-solubilized collagen in H. tubulosa (HT) and H. poli (HP). M – protein marker.

Morphometric data of H. polii according to length frequencies (N: 200).

Length Groups (cm)Length Fq. (%)Av. Length (cm)Av. Weight (g)Av. Gutted Weight (g)Sex & Ratio (%)Mean GSI (%)
FMFM
8.9–12.417.0010.95 ± 0.83a32.59 ± 5.87a23.14 ± 0.17a4-5.92 ± 0.05a-
12.5–14.125.0013.52 ± 0.97b44.73 ± 4.30ab29.63 ± 0.25b226.91 ± 0.04b4.89 ± 0.11a
14.2–15.930.0015.10 ± 1.06bc52.32 ± 6.55b38.74 ± 0.88c2-6.22 ± 0.03ab-
16.0–17.118.0016.43 ± 1.22cd76.44 ± 8.49c49.52 ± 0.31d-2-3.47 ± 0.04b
17.2–22.410.0019.12 ± 2.37d79.65 ± 7.94c52.78 ± 3.34d824.64 ± 0.15c-

Morphometric data of H. tubulosa according to length frequencies (N: 200).

Length Groups (cm)Length Fq. (%)Av. Length (cm)Av. Weight (g)Av. Gutted Weight (g)Sex & Ratio (%)Mean GSI (%)
FMFM
10.60 - 13.2920.0012.04 ± 1.01a50.91±5.28a33.65 ± 0.91a4-3.95 ± 0.09a-
13.30 - 14.2920.0013.83 ± 0.89b54.73±4.47ab36.63 ± 0.47ab6-5.52 ± 0.08b-
14.30 - 16.6922.0015.46 ± 1.26b64.72±5.60b39.34 ± 0.46b625.26 ± 0.01b2.67 ± 0.02a
16.70 - 18.3918.0017.08 ± 1.57bc80.23±9.28c45.12 ± 0.67c4-2.77 ± 0.05c-
18.40 - 25.0020.0021.17 ± 2.59c75.81±7.46cb45.64 ± 0.01c1026.55 ± 0.34d12.84 ± 0.12b
eISSN:
1897-3191
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Chemistry, other, Geosciences, Life Sciences
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