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Molecular identification and distribution of insect larvae in the Lower Danube River

Selma Menabit
Selma Menabit
, 
Lavinia Iancu
Lavinia Iancu
, 
Ana B. Pavel
Ana B. Pavel
, 
Adrian Popa
Adrian Popa
, 
Naliana Lupascu
Naliana Lupascu
 oraz 
Cristina Purcarea
Cristina Purcarea
   | 31 mar 2022
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Article Category: Original research paper
Data publikacji: 31 mar 2022
Zakres stron: 74 - 89
Otrzymano: 15 lip 2021
Przyjęty: 18 lis 2021
DOI: https://doi.org/10.26881/oahs.2022.1.07
Słowa kluczowe
© 2022 Selma Menabit et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Map of the sampling areas in the Lower Danube River (A) and the Danube Delta (B)
Map of the sampling areas in the Lower Danube River (A) and the Danube Delta (B)

Figure 2

Densities and distribution of insect larvae, depth (m) and oxygen records in the Lower Danube River
Densities and distribution of insect larvae, depth (m) and oxygen records in the Lower Danube River

Figure 2

Densities and distribution of insect larvae, depth (m) and oxygen records in the Lower Danube River
Densities and distribution of insect larvae, depth (m) and oxygen records in the Lower Danube River

Year of sampling, depth, substrate type, dissolved oxygen and presence of macrophytes

Crt. No.SitesYear of samplingDepth (m)Type of substrateDissolved oxygen mg/LMacrophytes presence (+/-)
Mean values/areaValue/site
Corabia area8.58
1.km 62620193.0sand8.90-
2.km 626.520207.3gravelly sand8.10-
3.km 627.520205.0sand8.81-
4.km 62920199.2gravelly sand8.82-
5.km 63020198.2gravelly sand8.04+
20208.67
6.km 63120195.3sand8.77-
Bechet area8.77
7.km 67420193.3sand8.75-
8.km 674.520193.2sand8.75+
9.km 67520194.0sand8.67-
10.km 675.520196.3sand8.67+
11.km 67620193.7gravelly sand9.31+
20208.10
12.km 676.520203.0sand8.15-
13.km 67820195.9sand9.78+
Pisculeţ area8.85
14.km 76020193.9sand9.36-
15.km 76120194.2sand9.61+
16.km 76220195.0sand8.95+
20208.99
17.km 762.520194.8gravelly sand8.96-
18.km 76420193.5sand8.55+
19.km 764.520194.9sand8.55+
20.km 76520192.8gravelly sand8.38+
20208.31
Cetate area7.80
21.km 80620193.8gravelly sand8.13-
22.km 80820195.0sand7.25-
23.km 81120197.8gravelly sand8.02-
Sulina area8.25
24.Old Danube meander20195.0sandy mud7.04-
25.NM 24(a) Sulina branch201916.5sand7.93-
26.NM 16 Sulina branch20195.2muddy sandy gravel7.90-
27.NM 8(a) Sulina branch201914.4sandy mud7.95-
28.NM 2 Sulina branch201917.5gravelly mud7.96-
29.NM 33 Sulina branch201912.5gravelly mud9.38-
30.NM 24(b) Sulina branch201912.5sand9.01-
31.NM 14(b) Sulina branch201913.5sandy mud9.55-
32.NM 8(b) Sulina branch (Meander)20194.3sandy mud9.07-
33.NM 14(c) Sulina branch (Crisan)201911.5sand7.95-
34.NM 14(a) Sulina branch (Crisan)201910.7gravelly mud7.95-
35.NM 21 Sulina channel20196.5sandy mud7.97-
36.Km 108(a) Ceatal Sf. Gheorghe20196.4sandy mud7.97-
37.Km 108(b) Ceatal Sf. Gheorghe20196.4gravelly mud7.97-

Sampling locations in the Lower Danube and density of larvae

Crt. No.SitesCoordinatesNumber of individuals/m2
Lat. (α)Long. (λ)G.f.H.f.E.v.A.e.H.b.T.b.S.m.
1.* km 81144°4’53.2”23°1’48.8”007.400
2.** km 80844°3’49.7”23°3’7.4”000050.400
3.** km 80644°2’55.3”23°2’14.5”0000226.800
4.** km 76543°48’18.2”22°57’34.3”0000403.200
5.0000025.20
6.** km 764.543°48’36.8”22°58’12.0”0000010080
7.** km 76443°48’5.7”22°58’35.5”000025.225.20
8.* km 762.543°48’37.7”22°59’52.7”7.4000162.80
9.* km 76243°47’54.0”23°0’2.4”029.600000
10.00004388.27.40
11.* km 76143°48’14.3”23°0’54.4”000007.40
12.** km 76043°48’9.8”23°1’51.1”0000201.600
13.* km 67843°44’40,6”23°57’52.9”007.407.400
14.* km 676.543°44’18.0”23°58’42.9”000096.200
15.* km 67643°44’13.0”23°59’4.3”000181300
16.00029.6000
17.* km 675.543°44’38,7”23°59’42,9”07.4007.47.40
18.* km 67543°44’5.5”23°59’45.1”007.40000
19.* km 674.543°44’3.0”24°0’8.1”066.607.4488.400
20.** km 67443°43’52.9”24°0’27.3”000025.200
21.* km 63143°46’8.3”24°29’58.8”096.200000
22.* km 63043°45’32.0”24°30’49.5”0429.214.80000
23.00001894.400
24.* km 62943°45’25.4”24°31’18.5”00007.400
25.** km 627.543°45’17.2”24°32’34.2”025.20025200
26.** km 626.543°45’46.6”24°33’30.4”0000100.800
27.* km 62643°45’37.5”24°33’48.9”29.67.400310.800
28.* km 108(a) Ceatal Sf. Gheorghe45°11’04.9”28°53’26.7”0000214.600
29.* km 108(b) Ceatal Sf. Ghoerghe45°11’04.39”28°53’26.7”00007.400
30.* NM 33 Sulina branch45°11’22.8”28°55’22.3”00007400
31.* NM 24 (a) Sulina branch45°10’28.5”29°03’19.4”0000384.800
32.* NM 24 (b) Sulina branch45°10’29.9”29°03’19.3”000062900
33.* NM 21 Sulina channel45010’54.2”29°10’19.1”00001420.800
34.* NM 16 Sulina branch45°10’33.3”29°19’05.5”0000651.200
35.* NM 14 (a) Sulina branch45°10’33.9”29°21’34.8”000037000
36.* NM14 (b) Sulina branch45°10’32.4”29°21’17.0”00001206.200
37.* NM 14 (c) Sulina branch (Crisan)45°10’30.9”29°21’39.6”000051800
38.* Old Danube (Meander)45°10’41.4”29°21’05.1”000007.40
39.* NM 8 (a) Sulina branch45°10’20.3”29°28’47.6”000014800
40.* NM 8 (b) Sulina branch45°10’39.4”29°28’33.2”00000014.8
41.* NM 2 Sulina branch45°09’39.6”29°36’31.6”000029600

Best match COI gene sequence of insect larvae species from the Danube River

Insect speciesIdentity (%)Cover (%)GenBank reference
E. viridulum99.84100.00MT298455.1
G. flavipes99.3698.00MT298633.1
T. bicolor99.8399.00KX143573.1
H. bulgaromanorum100.0098.00KX104210.1
H. flava99.8498.00KY262534.1
A. ephemerella98.6697.00LR135742.1
S. morio98.4197.00JX438311.1

Association of insect larvae with different types of substrate

Insect speciesOrderType of substrate
SandGravelly sandMuddy sandy gravelGravelly mudSandy mud
Erythromma viridulumOdonata++---
Gomphus flavipes++---
Triaenodes bicolor++--+
Hydropsyche bulgaromanorumTrichoptera+++++
Heptagenia flavaEphemeroptera++---
Acentria ephemerellaLepidoptera++---
Sialis morioMegaloptera----+
eISSN:
1897-3191
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Chemistry, other, Geosciences, Life Sciences
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