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Revista Romana de Medicina de Laborator's Cover Image
Revista Romana de Medicina de Laborator
Informacje o czasopiśmie
License
Format
Czasopismo
eISSN
2284-5623
Pierwsze wydanie
08 Aug 2013
Częstotliwość wydawania
4 razy w roku
Języki
Angielski
access type Otwarty dostęp

Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) for subgingival bacteriome identification in a group of treated periodontitis patients: a case series

Irina Lupșe,
Irina Lupșe,
Mirela Flonta,
Mirela Flonta,
Andreea Ciurea,
Andreea Ciurea,
Iulia Cristina Micu,
Iulia Cristina Micu,
Alexandra Roman,
Alexandra Roman,
Emoke Pall,
Emoke Pall,
Dora Maria Popescu oraz
Dora Maria Popescu oraz
Andrada Soancă
Andrada Soancă
Data publikacji: 09 May 2022
Tom & Zeszyt: Tom 30 (2022) - Zeszyt 2 (April 2022)
Zakres stron: 227 - 234
Otrzymano: 30 Oct 2021
Przyjęty: 10 Feb 2022
Revista Romana de Medicina de Laborator's Cover Image
Revista Romana de Medicina de Laborator
Informacje o czasopiśmie
License
Format
Czasopismo
eISSN
2284-5623
Pierwsze wydanie
08 Aug 2013
Częstotliwość wydawania
4 razy w roku
Języki
Angielski
Abstract

Periodontitis is a chronic multifactorial polymicrobial infection, characterized by profound modifications of the composition and proportion of the subgingival microbiota. Microbiological laboratory tests are sometimes used in periodontal diagnosis and monitoring of treatment, but both conventional cultivation methods and molecular techniques have some major drawbacks. Therefore, other performant bacterial identification methods must be considered. The aim of the current study was to use Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALTI-TOF MS) analysis in association with bacterial culture method to evaluate the modifications of the subgingival bacterial composition in periodontitis patients, before and after cause-related subgingival therapy. Subgingival plaque samples were collected from periodontal pockets before and after subgingival mechanical instrumentation and adjunctive local antimicrobial applications and were cultured in aerobic and anaerobic conditions. Microbial colonies were further assessed using MALDI-TOF-MS. A total of 36 bacterial strains were isolated from a group of 16 patients. All species from the orange complex were identified by MALDI-TOF MS. A marked reduction of detection frequency was observed in most bacterial strains, including the orange complex after cause-related periodontal treatment. The results of this study indicate that MALDI-TOF MS could be considered an accurate method for oral microbial identification and the cause-related periodontal treatment is useful for reducing the microbial burden.

Keywords

  • periodontitis
  • MALDI-TOF MS
  • bacteriome

1. Gully N, Bright R, Marino V, Marchant C, Cantley M, Haynes D, et al. Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis. PLoS One. 2014;9(6):e100838. DOI: 10.1371/journal.pone.010083810.1371/journal.pone.0100838406918024959715 Search in Google Scholar

2. Eke PI, Page RC, Wei L, Thornton-Evans G, Genco RJ. Update of the case definitions for population-based surveillance of periodontitis. J Periodontol. 2012;83(12):1449-54. DOI: 10.1902/jop.2012.11066410.1902/jop.2012.110664600537322420873 Search in Google Scholar

3. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol. 2005;43(11):5721-32. DOI: 10.1128/JCM.43.11.5721-5732.200510.1128/JCM.43.11.5721-5732.2005128782416272510 Search in Google Scholar

4. Jensen A, Ladegaard Grønkjær L, Holmstrup P, Vilstrup H, Kilian M. Unique subgingival microbiota associated with periodontitis in cirrhosis patients. Sci Rep. 2018;8(1):10718. DOI: 10.1038/s41598-018-28905-w10.1038/s41598-018-28905-w604806230013030 Search in Google Scholar

5. Van der Weijden GA, Timmerman MF, Reijerse E, Wolffe GN, Van Winkelhoff AJ, Van der Velden U. The prevalence of A. actinomycetemcomitans, P. gingivalis and P. intermedia in selected subjects with periodontitis. J Clin Periodontol. 1994;21(9):583-8. DOI: 10.1111/j.1600-051X.1994.tb00747.x10.1111/j.1600-051X.1994.tb00747.x7806673 Search in Google Scholar

6. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998;25(2):134-44. DOI: 10.1111/j.1600-051X.1998.tb02419.x10.1111/j.1600-051X.1998.tb02419.x Search in Google Scholar

7. Pérez-Chaparro PJ, Gonçalves C, Figueiredo LC, Faveri M, Lobão E, Tamashiro N, et al. Newly identified pathogens associated with periodontitis: a systematic review. J Dent Res. 2014;93(9):846-58. DOI: 10.1177/002203451454246810.1177/0022034514542468454110325074492 Search in Google Scholar

8. Sanz M, Herrera D, Kebschull M, Chapple I, Jepsen S, Beglundh T, et al. Treatment of stage I-III periodontitis-The EFP S3 level clinical practice guideline. J Clin Periodontol. 2020;47 Suppl 22:4-60. Search in Google Scholar

9. Eick S, Pfister W. Comparison of microbial cultivation and a commercial PCR based method for detection of periodontopathogenic species in subgingival plaque samples. J Clin Periodontol. 2002;29(7):638-44. DOI: 10.1034/j.1600-051X.2002.290708.x10.1034/j.1600-051X.2002.290708.x Search in Google Scholar

10. Clark AE, Kaleta EJ, Arora A, Wolk DM. Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology. Clin Microbiol Rev. 2013;26(3):547-603. DOI: 10.1128/CMR.00072-1210.1128/CMR.00072-12371949823824373 Search in Google Scholar

11. Saha S, Meenawat A, Sahu C, Srivastava V, Yadav S, Kumar V. Bacterial Identification and Monitoring Around Two-Piece Dental Implants by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS). J Lab Physicians. 2020;12(1):49-55. DOI: 10.1055/s-0040-171493410.1055/s-0040-1714934741917532792794 Search in Google Scholar

12. O’Leary TJ, Drake RB, Naylor JE. The plaque control record. J Periodontol. 1972;43(1):38. DOI: 10.1902/jop.1972.43.1.3810.1902/jop.1972.43.1.384500182 Search in Google Scholar

13. Yang NY, Zhang Q, Li JL, Yang SH, Shi Q. Progression of periodontal inflammation in adolescents is associated with increased number of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum. Int J Paediatr Dent. 2014;24(3):226-33. DOI: 10.1111/ipd.1206510.1111/ipd.1206524025042 Search in Google Scholar

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