<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Chicken bones, a by-product of the poultry industry, can directly or indirectly enter the food chain. Bone meal and bone products could be sources of many contaminants. Considering the wide range of uses made of bones in the culinary and food industries, this material needs to be safe and antibiotic residue–free. To determine if such is the case, the concentration of doxycycline in chicken bones was investigated, this antimicrobial being one of the most commonly used in poultry production.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>Ross 308 broilers were grouped into three experimental and one control group. Doxycycline was administered in drinking water at therapeutic and sub-therapeutic doses, as well as <italic>via</italic> spray treatment. The concentration of doxycycline in bones was determined post slaughter by ultra-high performance liquid chromatography–tandem mass spectrometry.</p>
</sec>
<sec>
<h3>Results</h3>
<p>Doxycycline was quantified at 135 μg/kg 22 days after the last day of antibiotic administration at therapeutic doses; 2,285 μg/kg after sub-therapeutic treatment for 27 days and 9.62 μg/kg 22 days after the end of spray application.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>High concentrations and long persistence of doxycycline in bones were found in this study. Doxycycline can contaminate all bone-derived products in the food and fertiliser industries.</p>
</sec>
</abstract>

Introduction
Chicken bones, a by-product of the poultry industry, can directly or indirectly enter the food chain. Bone meal and bone products could be sources of many contaminants. Considering the wide range of uses made of bones in the culinary and food industries, this material needs to be safe and antibiotic residue–free. To determine if such is the case, the concentration of doxycycline in chicken bones was investigated, this antimicrobial being one of the most commonly used in poultry production.


Material and Methods
Ross 308 broilers were grouped into three experimental and one control group. Doxycycline was administered in drinking water at therapeutic and sub-therapeutic doses, as well as via spray treatment. The concentration of doxycycline in bones was determined post slaughter by ultra-high performance liquid chromatography–tandem mass spectrometry.


Results
Doxycycline was quantified at 135 μg/kg 22 days after the last day of antibiotic administration at therapeutic doses; 2,285 μg/kg after sub-therapeutic treatment for 27 days and 9.62 μg/kg 22 days after the end of spray application.


Conclusion
High concentrations and long persistence of doxycycline in bones were found in this study. Doxycycline can contaminate all bone-derived products in the food and fertiliser industries.

Investigation of doxycycline residues in bones after oral administration to broiler chickens

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Chicken bones, a by-product of the poultry industry, can directly or indirectly enter the food chain. Bone meal and bone products could be sources...

Post-treatment day	Age of birds (days)	Group 1 DC mean concentration (± SD, μg/kg)	Group 2 DC mean concentration (± SD, μg/kg)	Group 3 DC mean concentration (± SD, μg/kg)
1	20	5,840 ± 843	132 ± 38	-
4	24	2,860 ± 410	83.1 ± 21	-
8	28	1,760 ± 322	45.5 ± 15	-
15	35	610 ± 150	41.6 ± 13	-
18	38	168 ± 84	16.4 ± 5.4	-
22	42	135 ± 51	9.62 ± 2.1	2285 ± 362

Investigation of doxycycline residues in bones after oral administration to broiler chickens

Data publikacji: 29 maj 2024

Zakres stron: -

Otrzymano: 28 gru 2023

Przyjęty: 22 maj 2024

DOI: https://doi.org/10.2478/jvetres-2024-0030

Słowa kluczowe
doxycycline, bones, broilers, UHPLC-MS/MS

© 2024 Anna Gajda et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Concentration of doxycycline in broiler bones following different routes of drug administration

Investigation of doxycycline residues in bones after oral administration to broiler chickens

Data publikacji: 29 maj 2024

Zakres stron: -

Otrzymano: 28 gru 2023

Przyjęty: 22 maj 2024

DOI: https://doi.org/10.2478/jvetres-2024-0030

Słowa kluczowedoxycycline, bones, broilers, UHPLC-MS/MS

© 2024 Anna Gajda et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Concentration of doxycycline in broiler bones following different routes of drug administration

Słowa kluczowe
doxycycline, bones, broilers, UHPLC-MS/MS