Primer name | Primer sequence | Reference |
---|---|---|
CEV qFor1 | 5′-AGTTTTGTAKATTGTAGCATTTCC-3′ | |
CEV qRev1 | 5′-GATTCCTCAAGGAGTTDCAGTAAA-3′ | 12 |
CEV qProbe1 | 5′-AGAGT TTGTTTCTTGCC ATACAAACT-3′ |
Sample collection site | Study material | Species | Symbol |
---|---|---|---|
Farm 2 | kidney, spleen | Carp ( |
DC1 |
Farm 2 | gills, skin | Carp ( |
DC1 |
Farm 2 | kidney, spleen | Carp ( |
DC2 |
Farm 2 | gills, skin | Carp ( |
DC2 |
Farm 2 | kidney, spleen | Carp ( |
DC4 |
Farm 2 | gills, skin | Carp ( |
DC4 |
Farm 2 | kidney, spleen | Carp ( |
DC7 |
Farm 2 | gills, skin | Carp ( |
DC7 |
Farm 2 | kidney, spleen | Carp ( |
DC8 |
Farm 2 | gills, skin | Carp ( |
DC8 |
Farm 2 | kidney, spleen | Carp ( |
DC9 |
Farm 2 | gills, skin | Carp ( |
DC9 |
Farm 9 | kidney, spleen | Carp ( |
9SK |
Farm 9 | gills, skin | Carp ( |
9SK |
Sample collection site | Code | Kidney | Spleen | Gills | Skin |
---|---|---|---|---|---|
GR 2 | DC1 | − | − | + | + |
GR 2 | DC2 | + | − | + | + |
GR 2 | DC4 | − | − | + | − |
GR 2 | DC7 | + | − | + | − |
GR 2 | DC8 | + | − | + | − |
GR 2 | DC9 | + | − | + | − |
GR 9 | 9SK | + | − | + | + |
No. | Sample code | Sample collection site | Threshold cycle |
---|---|---|---|
1 | DC 4 | Farm 2 | 36.67 |
2 | DC 7 | Farm 2 | 28.26 |
3 | DC 8 | Farm 2 | 24.44 |
4 | DC 9 | Farm 2 | 23.47 |
5 | DC 10 | Farm 2 | 25.08 |
6 | DC 11 | Farm 2 | 24.55 |
7 | DC12 | Farm 2 | 24.82 |
8 | DC 5 | Farm 5 | 28.2 |
Primer name | Primer sequence | Product size (base pairs) | Reference |
---|---|---|---|
CEV qFor1 | 5′-ATGGAGTATCCAAAGTACTTAG-3′ | 528 | |
CEV for B | |||
CEV rev J | 5′-CTCTTCACTATTGTGACTTTG-3′ | 528 | 12 |
CEV for B - int | 5′-GTTATCAATGAAATTTGTGTATTG-3′ | 478 | |
CEV rev J - int | 5′-TAGCAAAGTACTACCTCATCC-3′ | 478 |
Deionised water (PCR grade) | GoTaq G2 Green Master Mix | Forward primer | Reverse primer | Template DNA |
---|---|---|---|---|
6.5 mL | 12.5 mL | 0.5 mL | 0.5 mL | 5 mL |
Distilled water (PCR grade) | GoTaq G2 Green Master Mix | Forward primer | Reverse primer | TaqMan probe | Template DNA |
---|---|---|---|---|---|
6.25 mL | 12.5 mL | 0.5 mL | 0.5 mL | 0.25 mL | 5 mL |