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Study on the expression of testin in the testes of dogs

Rafał Ciaputa
Rafał Ciaputa
, 
Marcin Nowak
Marcin Nowak
, 
Stanisław Dzimira
Stanisław Dzimira
, 
Eleonora Brambilla
Eleonora Brambilla
, 
Małgorzata Kandefer-Gola
Małgorzata Kandefer-Gola
, 
Alicja Tomaszek
Alicja Tomaszek
, 
Aneta Popiel-Kopaczyk
Aneta Popiel-Kopaczyk
, 
Piotr Dzięgiel
Piotr Dzięgiel
 oraz 
Valeria Grieco
Valeria Grieco
   | 19 gru 2023
Journal of Veterinary Research's Cover Image
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Data publikacji: 19 gru 2023
Zakres stron: 627 - 633
Otrzymano: 17 maj 2023
Przyjęty: 03 paź 2023
DOI: https://doi.org/10.2478/jvetres-2023-0055
Słowa kluczowe
© 2023 Rafał Ciaputa et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Immunohistochemical examination of the expression of testin in the testes of dogs at various life stages. (A) Intense cytoplasmic testin expression in the Sertoli cells (SCs) and spermatids of an adult dog aged between 2 and 6 and weaker expression in the interstitium and interstitial endocrine Leydig cells. The lower left corner shows the negative control. (B) The same cells at higher magnification. (C) Very weak cytoplasmic reaction exclusively in the SCs of an older dog aged 9 or 10. The left corner shows the negative control. (D) Weaker expression of testin compared to normal adult canine testes in the SCs and interstitial endocrine Leydig cells of a puppy aged 2 months. The lower left corner shows the negative control. (E) Positive but weak cytoplasmic expression in the SCs of a miscarried canine foetus. The lower left corner shows the negative control. (A) and (C–E) scale bar 100 μm; (B) scale bar 50 μm
Immunohistochemical examination of the expression of testin in the testes of dogs at various life stages. (A) Intense cytoplasmic testin expression in the Sertoli cells (SCs) and spermatids of an adult dog aged between 2 and 6 and weaker expression in the interstitium and interstitial endocrine Leydig cells. The lower left corner shows the negative control. (B) The same cells at higher magnification. (C) Very weak cytoplasmic reaction exclusively in the SCs of an older dog aged 9 or 10. The left corner shows the negative control. (D) Weaker expression of testin compared to normal adult canine testes in the SCs and interstitial endocrine Leydig cells of a puppy aged 2 months. The lower left corner shows the negative control. (E) Positive but weak cytoplasmic expression in the SCs of a miscarried canine foetus. The lower left corner shows the negative control. (A) and (C–E) scale bar 100 μm; (B) scale bar 50 μm

Fig. 2.

Representative Western blot results of TES protein expression in testes. (A) A marked dark band between 55 kDa and 40 kDa corresponds to a testin protein of ~47 kDa. (B.1) Negative control without primary antibody. (B.2) Negative control with rabbit serum
Representative Western blot results of TES protein expression in testes. (A) A marked dark band between 55 kDa and 40 kDa corresponds to a testin protein of ~47 kDa. (B.1) Negative control without primary antibody. (B.2) Negative control with rabbit serum

Immunohistochemical results

  Germ cells/Spermatocytes Sertoli cells Leydig cells
% of positive cells Reaction intensity % of positive cells Reaction intensity % of positive cells Reaction intensity
Normal mature testes 4 3 4 3 3 2
Atrophic areas of aged canine testes 0 0 4 1 0 0
Normal immature testes 0 0 4 1 3 1
Testes of miscarried foetuses 0 0 2 1 0 0

Immunoreactive scores on the semiquantitative scale established by Remmele and Stegner (28)

Percentage of positive cells Points Immunohistochemical reaction Points
0 0 Absent 0
1–10% 1 Weak 1
11–50% 2 Moderate 2
5–80% 3 Intense 3
>80% 4    
eISSN:
2450-8608
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
Kanał RSS czasopisma