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Study on the expression of testin in the testes of dogs

Rafał Ciaputa

Rafał Ciaputa

Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Ciaputa, Rafał
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Marcin Nowak

Marcin Nowak

Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Nowak, Marcin
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Stanisław Dzimira

Stanisław Dzimira

Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Dzimira, Stanisław
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Eleonora Brambilla

Eleonora Brambilla

Department of Veterinary Medicine and Animal Science, University of MilanLodi, Italy
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Brambilla, Eleonora
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Małgorzata Kandefer-Gola

Małgorzata Kandefer-Gola

Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Kandefer-Gola, Małgorzata
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Alicja Tomaszek

Alicja Tomaszek

Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Tomaszek, Alicja
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Aneta Popiel-Kopaczyk

Aneta Popiel-Kopaczyk

Division of Histology and Embryology, Department of Human Morphology and Embryology, Wrocław Medical UniversityWrocław, Poland
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Popiel-Kopaczyk, Aneta
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Piotr Dzięgiel

Piotr Dzięgiel

Division of Histology and Embryology, Department of Human Morphology and Embryology, Wrocław Medical UniversityWrocław, Poland
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Dzięgiel, Piotr
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Valeria Grieco

Valeria Grieco

Department of Veterinary Medicine and Animal Science, University of MilanLodi, Italy
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Grieco, Valeria
  
19 dic 2023
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Pubblicato online: 19 dic 2023
Pagine: 627 - 633
Ricevuto: 17 mag 2023
Accettato: 03 ott 2023
DOI: https://doi.org/10.2478/jvetres-2023-0055
Parole chiave
© 2023 Rafał Ciaputa et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Immunohistochemical examination of the expression of testin in the testes of dogs at various life stages. (A) Intense cytoplasmic testin expression in the Sertoli cells (SCs) and spermatids of an adult dog aged between 2 and 6 and weaker expression in the interstitium and interstitial endocrine Leydig cells. The lower left corner shows the negative control. (B) The same cells at higher magnification. (C) Very weak cytoplasmic reaction exclusively in the SCs of an older dog aged 9 or 10. The left corner shows the negative control. (D) Weaker expression of testin compared to normal adult canine testes in the SCs and interstitial endocrine Leydig cells of a puppy aged 2 months. The lower left corner shows the negative control. (E) Positive but weak cytoplasmic expression in the SCs of a miscarried canine foetus. The lower left corner shows the negative control. (A) and (C–E) scale bar 100 μm; (B) scale bar 50 μm
Immunohistochemical examination of the expression of testin in the testes of dogs at various life stages. (A) Intense cytoplasmic testin expression in the Sertoli cells (SCs) and spermatids of an adult dog aged between 2 and 6 and weaker expression in the interstitium and interstitial endocrine Leydig cells. The lower left corner shows the negative control. (B) The same cells at higher magnification. (C) Very weak cytoplasmic reaction exclusively in the SCs of an older dog aged 9 or 10. The left corner shows the negative control. (D) Weaker expression of testin compared to normal adult canine testes in the SCs and interstitial endocrine Leydig cells of a puppy aged 2 months. The lower left corner shows the negative control. (E) Positive but weak cytoplasmic expression in the SCs of a miscarried canine foetus. The lower left corner shows the negative control. (A) and (C–E) scale bar 100 μm; (B) scale bar 50 μm

Fig. 2.

Representative Western blot results of TES protein expression in testes. (A) A marked dark band between 55 kDa and 40 kDa corresponds to a testin protein of ~47 kDa. (B.1) Negative control without primary antibody. (B.2) Negative control with rabbit serum
Representative Western blot results of TES protein expression in testes. (A) A marked dark band between 55 kDa and 40 kDa corresponds to a testin protein of ~47 kDa. (B.1) Negative control without primary antibody. (B.2) Negative control with rabbit serum

Immunohistochemical results

  Germ cells/Spermatocytes Sertoli cells Leydig cells
% of positive cells Reaction intensity % of positive cells Reaction intensity % of positive cells Reaction intensity
Normal mature testes 4 3 4 3 3 2
Atrophic areas of aged canine testes 0 0 4 1 0 0
Normal immature testes 0 0 4 1 3 1
Testes of miscarried foetuses 0 0 2 1 0 0

Immunoreactive scores on the semiquantitative scale established by Remmele and Stegner (28)

Percentage of positive cells Points Immunohistochemical reaction Points
0 0 Absent 0
1–10% 1 Weak 1
11–50% 2 Moderate 2
5–80% 3 Intense 3
>80% 4    
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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