<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Prior to the 2000s, swine dysentery was considered to be caused only by <italic>Brachyspira hyodysenteriae</italic> with contributing commensal intestinal anaerobes. Nowadays, it is known that the disease is caused by three strongly beta-haemolytic species of the anaerobic spirochaetal genus <italic>Brachyspira, i.e. B. hyodysenteriae</italic> and newly emerged <italic>B. hampsonii</italic> and <italic>B. suanatina.</italic></p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>The present investigation was carried out in November 2022 on nine Polish high-performing finisher pig farms. At every location one fresh pooled faecal sample was collected from 40 randomly selected pigs of between 60 and 110 kg live weight. Nucleic acid extracted from each pooled faecal sample was analysed by an in-house multiplex PCR for <italic>Brachyspira</italic> spp<italic>.</italic>, which is capable of confirming the <italic>Brachyspira</italic> genus and detecting and differentiating <italic>Brachyspira</italic> species.</p>
</sec>
<sec>
<h3>Results</h3>
<p>From a total of nine samples examined, the genetic material of <italic>B. suanatina</italic> was detected in seven. Non-pathogenic/questionably pathogenic <italic>Brachyspira</italic> spp. were found in six samples.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>To the best of our knowledge, this is the first report on the identification of <italic>B. suanatina</italic> in pigs outside Scandinavia, Germany and the United Kingdom. Our research not only provides valuable epidemiological data on <italic>B. suanatina</italic> infection in Europe but also highlights both the importance of modern laboratory diagnostics and the need for thorough investigation across regions, including retrospective studies.</p>
</sec>
</abstract>

Introduction
Prior to the 2000s, swine dysentery was considered to be caused only by Brachyspira hyodysenteriae with contributing commensal intestinal anaerobes. Nowadays, it is known that the disease is caused by three strongly beta-haemolytic species of the anaerobic spirochaetal genus Brachyspira, i.e. B. hyodysenteriae and newly emerged B. hampsonii and B. suanatina.


Material and Methods
The present investigation was carried out in November 2022 on nine Polish high-performing finisher pig farms. At every location one fresh pooled faecal sample was collected from 40 randomly selected pigs of between 60 and 110 kg live weight. Nucleic acid extracted from each pooled faecal sample was analysed by an in-house multiplex PCR for Brachyspira spp., which is capable of confirming the Brachyspira genus and detecting and differentiating Brachyspira species.


Results
From a total of nine samples examined, the genetic material of B. suanatina was detected in seven. Non-pathogenic/questionably pathogenic Brachyspira spp. were found in six samples.


Conclusion
To the best of our knowledge, this is the first report on the identification of B. suanatina in pigs outside Scandinavia, Germany and the United Kingdom. Our research not only provides valuable epidemiological data on B. suanatina infection in Europe but also highlights both the importance of modern laboratory diagnostics and the need for thorough investigation across regions, including retrospective studies.

First molecular detection of <italic>Brachyspira suanatina</italic> on pig farms in Poland

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

First molecular detection of  on pig farms in Poland

Introduction
Prior to the 2000s, swine dysentery was considered to be caused only by Brachyspira hyodysenteriae with contributing commensal intestinal...

First molecular detection of Brachyspira suanatina on pig farms in Poland

Data publikacji: 20 wrz 2023

Zakres stron: 353 - 359

Otrzymano: 12 mar 2023

Przyjęty: 19 cze 2023

DOI: https://doi.org/10.2478/jvetres-2023-0038

Słowa kluczowe
spp., PCR, pigs, Poland, swine dysentery

© 2023 Piotr Cybulski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

First molecular detection of Brachyspira suanatina on pig farms in Poland

Data publikacji: 20 wrz 2023

Zakres stron: 353 - 359

Otrzymano: 12 mar 2023

Przyjęty: 19 cze 2023

DOI: https://doi.org/10.2478/jvetres-2023-0038

Słowa kluczowespp., PCR, pigs, Poland, swine dysentery

© 2023 Piotr Cybulski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
spp., PCR, pigs, Poland, swine dysentery