First molecular detection of Brachyspira suanatina on pig farms in Poland
, , , oraz | 20 wrz 2023
O artykule
Data publikacji: 20 wrz 2023
Zakres stron: 353 - 359
Otrzymano: 12 mar 2023
Przyjęty: 19 cze 2023
DOI: https://doi.org/10.2478/jvetres-2023-0038
Słowa kluczowe
© 2023 Piotr Cybulski et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Farm descriptions
| Finisher farm | Farm capacity (thousand) | Source of animals (weaner farm) | Type of feed | Finisher farm location (province) |
|---|---|---|---|---|
| F1 | 19 | W1 | pelleted | West Pomeranian |
| F2 | 9 | W2 | pelleted | Pomeranian |
| F3 | 13 | W3 | pelleted | West Pomeranian |
| F4 | 12 | W3 | pelleted | West Pomeranian |
| F5 | 14 | W4 | liquid | West Pomeranian |
| F6 | 11 | W2 | pelleted | West Pomeranian |
| F7 | 7 | W5 | pelleted | West Pomeranian |
| F8 | 21 | W6 | pelleted | Greater Poland |
| F9 | 10 | W2 | pelleted | West Pomeranian |
Detection of different enteric pathogens in the study
| Farm | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Pathogen | Analysis | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | |
| PCR | + | + | + | + | + | + | + | + | + | ||
| PCR | − | − | − | − | − | − | − | − | − | ||
| PCR | + | + | + | − | + | − | + | + | + | ||
| PCR | − | − | − | − | − | − | − | − | − | ||
| PCR | − | + | + | + | − | − | − | − | + | ||
| PCR | − | + | − | − | + | + | − | + | + | ||
| PCR | + | − | − | − | − | − | − | − | − | ||
| PCR | − | − | − | − | − | − | − | − | − | ||
| Culture | − | − | SE | − | − | SE | SE | SE | − | ||
| PCR | n/a | n/a | + | n/a | n/a | + | + | + | n/a | ||
| Serovar Choleraesuis | PCR | n/a | n/a | − | n/a | n/a | − | + | − | n/a | |
| Serovar Typhimurium | PCR | n/a | n/a | − | n/a | n/a | − | − | − | n/a | |
| Viruses | TGEV | PCR | - | - | - | - | - | - | - | - | - |
| PEDV | PCR | − | − | − | − | − | − | − | − | − | |
| Parasites | Helminth eggs, | Sedimentation | − | − | − | − | − | − | − | − | − |
| encysted protozoa | and flotation | − | − | − | − | − | − | − | − | − | |
| Heine staining | − | − | − | − | − | − | − | − | − | ||
Targets for detection and differentiation of Brachyspira species by PCR
| Detection of | Target gene | Reference |
|---|---|---|
| 50S ribosomal protein L4 |
CP0196 |
|
| Bh100 |
( |
|
| hypothetic protein |
CVLB01000001* | |
| oxidoreductase |
CP019914 |
|
| methyl-accepting chemotaxis protein B |
CP002025 |
|
| hypothetic protein |
CP001959 |
|
| hypothetic protein |
CP002874 |
|
| ribulokinase |
ARQI01000130 |