<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease management may be more challenging if <italic>Escherichia coli</italic> produce extended-spectrum beta-lactamase (ESBL), which could cause resistance to aztreonam and third-generation cephalosporins. This study was aimed at determining the prevalence of the <italic>bla</italic><sub>CTX-M</sub> and <italic>bla</italic><sub>TEM</sub> genes among ESBL-producing <italic>E. coli</italic> isolated from broiler chickens in Indonesia.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>A total of 115 broiler cloacal swab samples were obtained from 22 farms and studied for the presence of <italic>E. coli</italic>. The isolates were identified using approved standard methods and were purified on eosin methylene blue agar media. The <italic>E. coli</italic> isolates were subjected to sensitivity testing using beta-lactam antibiotics, and ESBL production was confirmed by a double-disc synergy test. The presence of the <italic>bla</italic><sub>CTX-M</sub> and <italic>bla</italic><sub>TEM</sub> genes was identified using a PCR.</p>
</sec>
<sec>
<h3>Results</h3>
<p>It was found that 99/115 (86.1%) of the isolated <italic>E. coli</italic> were resistant to beta-lactam antibiotics and 34/115 (29.6%) of them were phenotypically detected to be ESBL producers. Of the 34 isolates that were confirmed ESBL producers, 32/34 (94.1%) of them harboured the <italic>bla</italic><sub>CTX-M</sub> and 13/34 (38.2%) the <italic>bla</italic><sub>TEM</sub> genes. The <italic>bla</italic><sub>CTX-M</sub> and <italic>bla</italic><sub>TEM</sub> genes were detected together in 12/34 (35.3%) isolates.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>This study discovered that broiler chickens are possible reservoirs of ESBL-producing <italic>E. coli</italic> that may infect humans. Thus, a committed public health education campaign is recommended in order to mitigate the potential threat to human health.</p>
</sec>
</abstract>

Introduction
Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease management may be more challenging if Escherichia coli produce extended-spectrum beta-lactamase (ESBL), which could cause resistance to aztreonam and third-generation cephalosporins. This study was aimed at determining the prevalence of the blaCTX-M and blaTEM genes among ESBL-producing E. coli isolated from broiler chickens in Indonesia.


Material and Methods
A total of 115 broiler cloacal swab samples were obtained from 22 farms and studied for the presence of E. coli. The isolates were identified using approved standard methods and were purified on eosin methylene blue agar media. The E. coli isolates were subjected to sensitivity testing using beta-lactam antibiotics, and ESBL production was confirmed by a double-disc synergy test. The presence of the blaCTX-M and blaTEM genes was identified using a PCR.


Results
It was found that 99/115 (86.1%) of the isolated E. coli were resistant to beta-lactam antibiotics and 34/115 (29.6%) of them were phenotypically detected to be ESBL producers. Of the 34 isolates that were confirmed ESBL producers, 32/34 (94.1%) of them harboured the blaCTX-M and 13/34 (38.2%) the blaTEM genes. The blaCTX-M and blaTEM genes were detected together in 12/34 (35.3%) isolates.


Conclusion
This study discovered that broiler chickens are possible reservoirs of ESBL-producing E. coli that may infect humans. Thus, a committed public health education campaign is recommended in order to mitigate the potential threat to human health.

Prevalence of the <italic>bla</italic><sub>CTX-M</sub> and <italic>bla</italic><sub>TEM</sub> genes among extended-spectrum beta lactamase–producing <italic>Escherichia coli</italic> isolated from broiler chickens in Indonesia

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence of the  and  genes among extended-spectrum beta lactamase–producing  isolated from broiler chickens in Indonesia

Introduction
Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease...

Prevalence of the bla_CTX-M and bla_TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Data publikacji: 16 cze 2023

Zakres stron: 179 - 186

Otrzymano: 11 sty 2023

Przyjęty: 19 kwi 2023

DOI: https://doi.org/10.2478/jvetres-2023-0025

Słowa kluczowe
ESBL-producing, broiler chickens, human health

© 2023 Hayyun Durrotul Faridah et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence of the blaCTX-M and blaTEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Data publikacji: 16 cze 2023

Zakres stron: 179 - 186

Otrzymano: 11 sty 2023

Przyjęty: 19 kwi 2023

DOI: https://doi.org/10.2478/jvetres-2023-0025

Słowa kluczoweESBL-producing, broiler chickens, human health

© 2023 Hayyun Durrotul Faridah et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence of the bla_CTX-M and bla_TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia

Słowa kluczowe
ESBL-producing, broiler chickens, human health