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Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism


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Fig. 1.

Morphological analysis of corpus luteum tissues of full-sized Yorkshire pigs and mini-pigs using haematoxlin and eosin staining, showing the distribution of large corpus luteum cells and small corpus luteum cells in both pig species
Morphological analysis of corpus luteum tissues of full-sized Yorkshire pigs and mini-pigs using haematoxlin and eosin staining, showing the distribution of large corpus luteum cells and small corpus luteum cells in both pig species

Fig. 2.

Genes related to apoptosis, cell reorganisation and hormones in the proteins and mRNAs of the corpus luteum tissues of Yorkshire pigs (Y-pig) and mini-pigs (M-pig). A – immunohistogram of caspase 3 (Casp-3) protein in the corpus luteum tissue; B – Western blot of cell death factors and zymography of the activity of matrix metalloproteinases (MMPs) involved in cell reorganisation; C – real-time PCR analysis of messenger RNA involved in apoptosis; D – detection of hormone receptors and proteins involved in cell reorganisation. BCL-2 – B cell lymphoma 2 protein; BAX – Bcl-2-associated X protein; 20α-HSD – 20alpha-hydroxysteroid dehydrogenase; TIMP – tissue inhibitor of metalloproteinases; OD – optical density; IGF – insulin-like growth factor; mTOR – mammalian target of rapamycin; P4 – progesterone; LH – luteinising hormone; TNF – tumour necrosis factor
Genes related to apoptosis, cell reorganisation and hormones in the proteins and mRNAs of the corpus luteum tissues of Yorkshire pigs (Y-pig) and mini-pigs (M-pig). A – immunohistogram of caspase 3 (Casp-3) protein in the corpus luteum tissue; B – Western blot of cell death factors and zymography of the activity of matrix metalloproteinases (MMPs) involved in cell reorganisation; C – real-time PCR analysis of messenger RNA involved in apoptosis; D – detection of hormone receptors and proteins involved in cell reorganisation. BCL-2 – B cell lymphoma 2 protein; BAX – Bcl-2-associated X protein; 20α-HSD – 20alpha-hydroxysteroid dehydrogenase; TIMP – tissue inhibitor of metalloproteinases; OD – optical density; IGF – insulin-like growth factor; mTOR – mammalian target of rapamycin; P4 – progesterone; LH – luteinising hormone; TNF – tumour necrosis factor

Fig. 3.

Comparative analysis of expression patterns of major marker proteins and mRNAs related to the occurrence of autophagy in Yorkshire pigs (Y-pig) and mini-pigs (M-pig)). A – expression pattern of MAP1LC3A, shown byimmunohistochemistry; B – expression of autophagy-related proteins compared by Western blotting; C – analysis of autophagy-related gene expression by real-time PCR
Comparative analysis of expression patterns of major marker proteins and mRNAs related to the occurrence of autophagy in Yorkshire pigs (Y-pig) and mini-pigs (M-pig)). A – expression pattern of MAP1LC3A, shown byimmunohistochemistry; B – expression of autophagy-related proteins compared by Western blotting; C – analysis of autophagy-related gene expression by real-time PCR

Primers used for real-time PCR analysis of apoptosis-associated genes

Primer name Sequence GenBank gene ID
Porcine GAPDH F 5′ CCCGTTCGACAGACAGCCGTG 3′ NM_001206359.1
Porcine GAPDH R 5′ CCGCCTTGACTGTGCCGTGG 3′
Porcine BCL-2 F 5′ AGCATGCGGCCTCTATTTGA 3′ XM_021099593.1
Porcine BCL-2 R 5′ GGCCCGTGGACTTCACTTAT 3′
Porcine Casp-3 F 5′ CATGGTCAGGCCTTGTGAAGCTGAC 3′ NM_214131.1
Porcine Casp-3 R 5′ TCTTCTTCATGACCTCACCGTCGGG 3′
Porcine BAX F 5′ TGCTTCAGGGTTTCATCCAG 3′ AM233489.1
Porcine BAX R 5′ GTGTCCCAAAGTAGGAGAGG 3′
Porcine 20α-HSD F 5′ GCCATTGCCAAAAAGCACAAG 3′ NM_001044618.1
Porcine 20α-HSD R 5′ GGAAAGCGGATAGTCAGGGTGATC 3′
Porcine ATG1 F 5′ CAGCGCATTGAGCAGAACCTCAGT 3′ NM_140344.3
Porcine ATG1 R 5′ GATGGTTCCGACTTGGGGAGATGGT 3′
Porcine ATG13 F 5′ ATGGAAGCTGATCTCAATTCCCAGG 3′ XM_005660951.3
Porcine ATG13 R 5′ TTACTGCAGGGTTTCCACAAAGGCA 3′
Porcine ATG5 F 5′ AGAGAAGTCTGTCCTTCCGCAGTCG 3′ NM_001037152.2
Porcine ATG5 R 5′ AAGCAGAAGGGTGACATGCTCTGGT 3′
Porcine MAP1LC3A F 5′ AGAAGCAGCTGCCAGTCCTGGACA 3′ GU272221.1
Porcine MAP1LC3A R 5′ CAGGCAGGCCTGAGCAATCTTTATT 3′
Porcine Beclin-1 F 5′ TGGCGGAAAATCTCGAGAAGGTCCA 3′ CX061365.1
Porcine Beclin-1 R 5′ TGTGCCAAATTGTCCACTGTGCCAA 3′
eISSN:
2450-8608
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine