Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism
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16 jun 2023
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Publicado en línea: 16 jun 2023
Páginas: 307 - 314
Recibido: 19 oct 2022
Aceptado: 14 abr 2023
DOI: https://doi.org/10.2478/jvetres-2023-0023
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© 2023 Ji-Hye Lee et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
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Fig. 3.
Primers used for real-time PCR analysis of apoptosis-associated genes
| Primer name | Sequence | GenBank gene ID |
|---|---|---|
| Porcine GAPDH F | 5′ CCCGTTCGACAGACAGCCGTG 3′ | NM_001206359.1 |
| Porcine GAPDH R | 5′ CCGCCTTGACTGTGCCGTGG 3′ | |
| Porcine BCL-2 F | 5′ AGCATGCGGCCTCTATTTGA 3′ | XM_021099593.1 |
| Porcine BCL-2 R | 5′ GGCCCGTGGACTTCACTTAT 3′ | |
| Porcine Casp-3 F | 5′ CATGGTCAGGCCTTGTGAAGCTGAC 3′ | NM_214131.1 |
| Porcine Casp-3 R | 5′ TCTTCTTCATGACCTCACCGTCGGG 3′ | |
| Porcine BAX F | 5′ TGCTTCAGGGTTTCATCCAG 3′ | AM233489.1 |
| Porcine BAX R | 5′ GTGTCCCAAAGTAGGAGAGG 3′ | |
| Porcine 20α-HSD F | 5′ GCCATTGCCAAAAAGCACAAG 3′ | NM_001044618.1 |
| Porcine 20α-HSD R | 5′ GGAAAGCGGATAGTCAGGGTGATC 3′ | |
| Porcine ATG1 F | 5′ CAGCGCATTGAGCAGAACCTCAGT 3′ | NM_140344.3 |
| Porcine ATG1 R | 5′ GATGGTTCCGACTTGGGGAGATGGT 3′ | |
| Porcine ATG13 F | 5′ ATGGAAGCTGATCTCAATTCCCAGG 3′ | XM_005660951.3 |
| Porcine ATG13 R | 5′ TTACTGCAGGGTTTCCACAAAGGCA 3′ | |
| Porcine ATG5 F | 5′ AGAGAAGTCTGTCCTTCCGCAGTCG 3′ | NM_001037152.2 |
| Porcine ATG5 R | 5′ AAGCAGAAGGGTGACATGCTCTGGT 3′ | |
| Porcine MAP1LC3A F | 5′ AGAAGCAGCTGCCAGTCCTGGACA 3′ | GU272221.1 |
| Porcine MAP1LC3A R | 5′ CAGGCAGGCCTGAGCAATCTTTATT 3′ | |
| Porcine Beclin-1 F | 5′ TGGCGGAAAATCTCGAGAAGGTCCA 3′ | CX061365.1 |
| Porcine Beclin-1 R | 5′ TGTGCCAAATTGTCCACTGTGCCAA 3′ |