Variable | Seroprevalence | RT-PCR positive | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
% | 95% CI | P | % | 95% CI | χ2 | P | ||||
Age group* | 23.20 | <0.001 | 5.06 | 0.080 | ||||||
≤ 3 months | 16/89 | 82.0 | 73.9–90.1 | 14/89 | 15.7 | 8.0–23.4 | ||||
3–6 months | 102/126 | 81.0 | 74.0–87.9 | 12/126 | 9.5 | 4.3–14.7 | ||||
≥ 6 months | 25/51 | 49.0 | 34.8–63.2 | 2/51 | 3.9 | −1.6–9.4 | ||||
All | 201/261 | 77.0 | 71.4–82.0 | 29/261 | 11.1 | 7.6–15.6 | ||||
Sex | 0.006 | 0.940 | 2.90 | 0.088 | ||||||
Female | 89/120 | 74.2 | 65.6–87.1 | 7/120 | 5.8 | 2.4–11.6 | ||||
Male | 25/34 | 73.5 | 55.6–87.1 | 5/34 | 14.7 | 5.0–31.1 | ||||
All | 114/154 | 74.0 | 66.4–80.8 | 12/154 | 7.8 | 4.1–13.2 | ||||
Respiratory signs | 4.39 | 0.0361 | 3.23 | 0.072 | ||||||
Yes | 62/86 | 72.1 | 61.4–81.2 | 15/86 | 17.4 | 10.1–27.1 | ||||
No | 57/85 | 67.1 | 56.0–76.9 | 7/85 | 8.2 | 3.4–16.2 | ||||
All | 119/171 | 69.6 | 62.1–76.4 | 22/171 | 12.9 | 8.2–18.8 | ||||
BoHV–1 status | 13.97 | <0.001 | 0.084 | 9.772 | ||||||
Seropositive | 99/117 | 84.6 | 76.8–90.6 | 13/117 | 11.1 | 6.1–18.3 | ||||
Seronegative | 116/179 | 64.8 | 57.3–71.8 | 18/179 | 10.1 | 6.1–15.4 | ||||
All | 215/296 | 72.6 | 67.2–77.6 | 31/296 | 10.5 | 7.2–14.5 | ||||
BVDV status | 5.98 | 0.014 | 1.249 | 0.264 | ||||||
Seropositive | 113/144 | 78.5 | 70.9–84.9 | 18/144 | 12.5 | 7.6–19.0 | ||||
Seronegative | 93/142 | 65.5 | 57.1–73.3 | 12/142 | 8.4 | 4.4–14.3 | ||||
All | 206/286 | 72.0 | 66.4–77.2 | 30/286 | 10.5 | 7.2–14.6 | ||||
BoHV–1 PCR | 1.142 | 0.285 | 0.35 | 0.554 | ||||||
Positive | 3/3 | 100.0 | 29.2–100.0 | 0/3 | 0.0 | 0.0–70.8 | ||||
Negative | 212/293 | 72.3 | 66.9–77.4 | 31/293 | 10.6 | 7.3–14.7 | ||||
All | 215/296 | 72.6 | 76.2–77.6 | 31/296 | 10.5 | 7.2–14.5 | ||||
BVDV RT–PCR | 5.107 | 0.024 | 0.04 | 0.841 | ||||||
Positive | 3/8 | 37.5 | 8.5–75.5 | 1/8 | 12.5 | 0.3–52.7 | ||||
Negative | 212/288 | 73.6 | 68.1–78.6 | 30/288 | 10.4 | 7.1–14.5 | ||||
All | 215/296 | 72.6 | 67.2–77.6 | 31/296 | 10.5 | 7.2–14/5 | ||||
BCoV RT–PCR | 0.042 | 0.837 | ||||||||
Positive | 23/31 | 74.2 | 55.4–88.1 | |||||||
Negative | 192/265 | 72.4 | 66.7–77.7 | |||||||
All | 215/296 | 72.6 | 67.2–77.6 | |||||||
Herd size* | 29.34 | <0.001 | 10.35 | 0.006 | ||||||
Smaller (≤77) | 31/62 | 50.0 | 6.0–62.8 | 1/62 | 1.6 | −1.6–4.8 | ||||
Medium (80–590) | 128/158 | 81.0 | 74.8–87.2 | 19/158 | 12.0 | 6.9–17.1 | ||||
Larger (≥750) | 24/25 | 96.0 | 87.7–104.2 | 6/25 | 24.0 | 6.0–42.0 | ||||
All | 183/245 | 74.7 | 69.1–80.3 | 25/245 | 10.2 | 6.7–14.8 | ||||
Province | 12.65 | 0.013 | 13.04 | 0.011 | ||||||
Mazowieckie | 44/66 | 66.7 | 54.0–77.8 | 9/66 | 13.6 | 6.4–24.3 | ||||
Pomorskie | 42/60 | 70.0 | 56.8–81.1 | 2/60 | 3.3 | 0.4–11.5 | ||||
Opolskie | 58/70 | 82.8 | 71.9–90.8 | 5/70 | 7.1 | 2.4–15.9 | ||||
Podlaskie | 38/45 | 84.4 | 70.5–93.5 | 3/45 | 6.7 | 1.4–18.3 | ||||
Wielkopolskie | 33/55 | 60.0 | 46.0–73.0 | 12/55 | 21.8 | 11.8–35.0 | ||||
All | 215/296 | 72.6 | 67.2–77.6 | 31/296 | 10.5 | 7.2–14.5 |
Variable | Category | Odds ratio (OR) | β (SE) | P |
95% CI | |
---|---|---|---|---|---|---|
Herd size* | ||||||
Smaller (≤77) | reference | |||||
Medium (80–590) | 4.22 | 1.92 | 3.16 | 0.004 | 1.73–10.30 | |
Larger (≥750) | 0.02 | 0.02 | -4.24 | <0.001 | 0.002–0.11 | |
Fixed effect | Variance | β (SE)c | 95% CI | |||
Age group | 1.06 | 1.66 | 0.05–23.16 |
Target | Primer/probe | Sequence (5′–3′) | Amplicon size (bp) | Gene/ protein | Reference |
---|---|---|---|---|---|
BCoV-F | CTGGAAGTTGGTGGAGTT | ||||
BCoV-R | ATTATCGGCCTAACATACATC | 85 | M/matrix | ||
BCoV | BCoV-Pb | FAM-CCTTCATATCTATACACATCAAGTTGTT-BHQ1 | ( |
||
Sp1 | CTTATAAGTGCCCCCAAACTAAAT | ||||
Sp2 | CCTACTGTGAGATCACATGTTTG | 622 | S/spike | ||
Pesti-F | CTAGCCATGCCCTTAGTAG | ||||
Pesti-R | CGTCGAACCAGTGACGACT | ||||
BVDV | BVDV1 | FAM-TAGCAACAGTGGTGAGTTCGTTGGATGGCT-BHQ1 | 106 | 5′-UTR | ( |
BVDV2 | TxR-TAGCGGTAGCAGTGAGTTCGTTGGATGGCC-BHQ1 | ||||
gD5595-F | CCGCCGTATTTTGAGGAGTCG | ||||
BoHV-1 | gD5704-R | TCGGTCTCCCCTTCRTCCTC | 46 | gD | ( |
BHV1-gD-FAM* | FAM-TCGGTCTCCCCTTCRTCCTC-BHQ1 | ||||
ACT-1005-F | CAGCACAATGAAGATCAAGATCATC | ||||
β Actin | ACT-1135-R | CGGACTCATCGTACTCCTGCTT | 130 | bACT | ( |
ACT-1081-HEX | HEX-TCGCTGTCCACCTTCCAGCAGATGT- BHQ1 |
Variable | Category | Odds ratio | β (SE) | P |
95% CI | |
---|---|---|---|---|---|---|
Age group* | ||||||
≤3 months | reference | |||||
3–6 months | 0.58 | 0.32 | −0.99 | 0.323 | 0.19–1.72 | |
≥6 months | 0.27 | 0.14 | −2.44 | 0.015 | 0.10–0.78 | |
Herd size* | ||||||
Smaller (≤77) | reference | |||||
Medium (80–590) | 5.20 | 2.93 | 2.91 | 0.004 | 1.71–15.72 | |
Larger (≥750) | 39.90 | 45.78 | 3.21 | 0.001 | 4.21–378.14 | |
Fixed effect | Variance | β (SE) | 95% CI | |||
Province | 0.95 | 0.87 | 0.16–5.76 |