<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2021-0016_s_005">
<h3>Introduction</h3>
<p>Inflammation is one of the main causes of impaired health in livestock and some of its processes weaken animal productivity and impact human health. The present study was conducted to evaluate the effect of echinacea extract (cichoric acid – CA) on yak peripheral blood mononuclear cells (PBMCs), inflammatory-related factors, and the toll-like receptor (TLR)4 signalling pathway induced by lipopolysaccharide (LPS) in these PBMCs.</p></sec>
<sec id="j_jvetres-2021-0016_s_006">
<h3>Material and Methods</h3>
<p>Yak PBMCs were co-cultured with LPS and CA <italic>in vitro</italic>. The proliferative activity of cells was detected using the cell-counting kit-8 method, the optimal stimulation concentration of LPS was selected, the effect of CA on the content of inflammation-related factors was evaluated using an ELISA kit, and the mRNA expression of these factors was detected by RT-PCR.</p></sec>
<sec id="j_jvetres-2021-0016_s_007">
<h3>Results</h3>
<p>CA inhibited the inflammatory response of yak PBMCs induced by LPS. CA inhibited gene and protein expression of key nodes of the TLR4 signalling pathway in yak PBMCs.</p></sec>
<sec id="j_jvetres-2021-0016_s_008">
<h3>Conclusion</h3>
<p>It is suggested that CA has anti-inflammatory and immunomodulatory effects on yak PBMCs <italic>via</italic> the TLR4 pathway.</p></sec>
</abstract>

Introduction
Inflammation is one of the main causes of impaired health in livestock and some of its processes weaken animal productivity and impact human health. The present study was conducted to evaluate the effect of echinacea extract (cichoric acid – CA) on yak peripheral blood mononuclear cells (PBMCs), inflammatory-related factors, and the toll-like receptor (TLR)4 signalling pathway induced by lipopolysaccharide (LPS) in these PBMCs.

Material and Methods
Yak PBMCs were co-cultured with LPS and CA in vitro. The proliferative activity of cells was detected using the cell-counting kit-8 method, the optimal stimulation concentration of LPS was selected, the effect of CA on the content of inflammation-related factors was evaluated using an ELISA kit, and the mRNA expression of these factors was detected by RT-PCR.

Results
CA inhibited the inflammatory response of yak PBMCs induced by LPS. CA inhibited gene and protein expression of key nodes of the TLR4 signalling pathway in yak PBMCs.

Conclusion
It is suggested that CA has anti-inflammatory and immunomodulatory effects on yak PBMCs via the TLR4 pathway.

<italic>Echinacea purpurea</italic> extract (cichoric acid) exerts an anti-inflammatory effect on yak PBMCs and regulates the TLR4 signalling pathway

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 extract (cichoric acid) exerts an anti-inflammatory effect on yak PBMCs and regulates the TLR4 signalling pathway

Introduction
Inflammation is one of the main causes of impaired health in livestock and some of its processes weaken animal productivity and impact human...

Echinacea purpurea extract (cichoric acid) exerts an anti-inflammatory effect on yak PBMCs and regulates the TLR4 signalling pathway

Data publikacji: 09 mar 2021

Zakres stron: 109 - 115

Otrzymano: 06 lip 2020

Przyjęty: 23 lut 2021

DOI: https://doi.org/10.2478/jvetres-2021-0016

Słowa kluczowe
CA, yak PBMCs, inflammation, TLR4, LPS

© 2021 C.H. Xue et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Echinacea purpurea extract (cichoric acid) exerts an anti-inflammatory effect on yak PBMCs and regulates the TLR4 signalling pathway

Data publikacji: 09 mar 2021

Zakres stron: 109 - 115

Otrzymano: 06 lip 2020

Przyjęty: 23 lut 2021

DOI: https://doi.org/10.2478/jvetres-2021-0016

Słowa kluczoweCA, yak PBMCs, inflammation, TLR4, LPS

© 2021 C.H. Xue et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
CA, yak PBMCs, inflammation, TLR4, LPS