Otwarty dostęp

Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing

Dulce Anahy Verdugo Escárcega
Dulce Anahy Verdugo Escárcega
, 
Claudia Angélica Perea Razo
Claudia Angélica Perea Razo
, 
Sara González Ruíz
Sara González Ruíz
, 
Susana Lucia Sosa Gallegos
Susana Lucia Sosa Gallegos
, 
Feliciano Milián Suazo
Feliciano Milián Suazo
 oraz 
Germinal Jorge Cantó Alarcón
Germinal Jorge Cantó Alarcón
   | 14 lut 2020
Journal of Veterinary Research's Cover Image
O artykule
Poprzedni artykuł
Następny artykuł
Zacytuj
Data publikacji: 14 lut 2020
Zakres stron: 51 - 61
Otrzymano: 10 maj 2019
Przyjęty: 03 lut 2020
DOI: https://doi.org/10.2478/jvetres-2020-0010
Słowa kluczowe
© 2020 D.A.V. Escárcega et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Map of the state of Jalisco, Mexico, indicating the three municipalities (Arandas, San Miguel el Alto, and Tepatitlán de Morelos) included in this study
Map of the state of Jalisco, Mexico, indicating the three municipalities (Arandas, San Miguel el Alto, and Tepatitlán de Morelos) included in this study

Fig. 2

Spoligoforest including all the M. bovis spoligotypes obtained from Holstein cattle in the region of Altos Sur of Jalisco, Mexico. The size of each node is an increasing function of the number of isolates (i.e., the cluster size); edges between nodes reflect evolutionary relationships between spoligotypes with arrowheads pointing to descendants
Spoligoforest including all the M. bovis spoligotypes obtained from Holstein cattle in the region of Altos Sur of Jalisco, Mexico. The size of each node is an increasing function of the number of isolates (i.e., the cluster size); edges between nodes reflect evolutionary relationships between spoligotypes with arrowheads pointing to descendants

Fig. 3

Distribution of M. bovis spoligotypes by municipality in the region of Altos Sur of Jalisco. The total number of isolates per spoligotype is in parentheses
Distribution of M. bovis spoligotypes by municipality in the region of Altos Sur of Jalisco. The total number of isolates per spoligotype is in parentheses

Fig. 4

Evolutionary analysis by maximum likelihood using as input the concatenated SNP sequences from WGS of 34 M. bovis isolates from three municipalities of the region Altos Sur of Jalisco, Mexico: (T) Tepatitlán de Morelos, (A) Arandas, and (M) San Miguel el Alto. The tree is rooted to the reference M. bovis AF2122/97 (accession number NC_002945.4)
Evolutionary analysis by maximum likelihood using as input the concatenated SNP sequences from WGS of 34 M. bovis isolates from three municipalities of the region Altos Sur of Jalisco, Mexico: (T) Tepatitlán de Morelos, (A) Arandas, and (M) San Miguel el Alto. The tree is rooted to the reference M. bovis AF2122/97 (accession number NC_002945.4)

Fig. 5

Maximum likelihood phylogenetic reconstruction of the whole-genome SNP sequences of 196 M. bovis isolates from Mexico (Altos Sur isolates are in red)
Maximum likelihood phylogenetic reconstruction of the whole-genome SNP sequences of 196 M. bovis isolates from Mexico (Altos Sur isolates are in red)

Prevalence of BTb by municipality as confirmed by positive isolation of M. bovis in culture

Municipality Positive cases Negative cases Total animals sampled Regional prevalence Total prevalence
Tepatitlán de Morelos 33 197 230 14.3 9.9
San Miguel el Alto 9 38 47 19.1 2.7
Arandas 8 47 55 14.5 2.4
TOTAL 50 282 332

Spoligotype patterns obtained for isolates of M. bovis from the region of Altos Sur in Jalisco, Mexico. The total number of isolates per spoligotype is in parentheses

Type of tissue sampled which showed typical BTb lesions

Type of tissue Number of samples with lesions %
Lymph nodes
      Maxillary 2 2.9
      Retropharyngeal 18 26.4
      Mediastinal 22 32.4
Lung 3 4.4
Liver 1 1.5
Multiple tissue lesions
      retropharyngeal and maxillary 1 1.5
      retropharyngeal and mediastinal 9 13.2
      retropharyngeal and lung 3 4.4
      retropharyngeal, mediastinal and lung 4 5.9
      maxillary and mediastinal 1 1.5
      mediastinal and lung 4 5.9
TOTAL 68 100

Genetic diversity within the three major clades obtained by WGS of M. bovis isolates from the region of Altos Sur in Jalisco, Mexico

Clade Isolates Spoligotypes Municipalities
I 16 SB0673, SB0971, NF Tepatitlán de Morelos, Arandas, San Miguel el Alto
II 8 SB0130, SB0145, SB1758, SB1216, SB0872 Tepatitlán de Morelos, Arandas, San Miguel el Alto
III 10 SB0121, SB1177 Tepatitlán de Morelos, Arandas, San Miguel el Alto

Contribution of the total number of samples by municipality

Municipality Number of samples Percentage of the total sample size
Tepatitlán de Morelos 230 69
Arandas 55 17
San Miguel el Alto 47 14
TOTAL 332 100
eISSN:
2450-8608
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
Kanał RSS czasopisma