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Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing

Dulce Anahy Verdugo Escárcega

Dulce Anahy Verdugo Escárcega

Master’s Programme in Sustainable Animal Health and Production, Autonomous University of QueretaroQueretaro, Mexico
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Escárcega, Dulce Anahy Verdugo
, 
Claudia Angélica Perea Razo

Claudia Angélica Perea Razo

Doctorate Programme in Biological Sciences, Autonomous University of QueretaroQueretaro, Mexico
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Razo, Claudia Angélica Perea
, 
Sara González Ruíz

Sara González Ruíz

Doctorate Programme in Biological Sciences, Autonomous University of QueretaroQueretaro, Mexico
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Ruíz, Sara González
, 
Susana Lucia Sosa Gallegos

Susana Lucia Sosa Gallegos

Natural Sciences Department, Autonomous University of QueretaroQueretaro, Mexico
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Gallegos, Susana Lucia Sosa
, 
Feliciano Milián Suazo

Feliciano Milián Suazo

Natural Sciences Department, Autonomous University of QueretaroQueretaro, Mexico
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Suazo, Feliciano Milián
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Germinal Jorge Cantó Alarcón

Germinal Jorge Cantó Alarcón

Natural Sciences Department, Autonomous University of QueretaroQueretaro, Mexico
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Alarcón, Germinal Jorge Cantó
  
14 feb 2020
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Pubblicato online: 14 feb 2020
Pagine: 51 - 61
Ricevuto: 10 mag 2019
Accettato: 03 feb 2020
DOI: https://doi.org/10.2478/jvetres-2020-0010
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© 2020 D.A.V. Escárcega et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Map of the state of Jalisco, Mexico, indicating the three municipalities (Arandas, San Miguel el Alto, and Tepatitlán de Morelos) included in this study
Map of the state of Jalisco, Mexico, indicating the three municipalities (Arandas, San Miguel el Alto, and Tepatitlán de Morelos) included in this study

Fig. 2

Spoligoforest including all the M. bovis spoligotypes obtained from Holstein cattle in the region of Altos Sur of Jalisco, Mexico. The size of each node is an increasing function of the number of isolates (i.e., the cluster size); edges between nodes reflect evolutionary relationships between spoligotypes with arrowheads pointing to descendants
Spoligoforest including all the M. bovis spoligotypes obtained from Holstein cattle in the region of Altos Sur of Jalisco, Mexico. The size of each node is an increasing function of the number of isolates (i.e., the cluster size); edges between nodes reflect evolutionary relationships between spoligotypes with arrowheads pointing to descendants

Fig. 3

Distribution of M. bovis spoligotypes by municipality in the region of Altos Sur of Jalisco. The total number of isolates per spoligotype is in parentheses
Distribution of M. bovis spoligotypes by municipality in the region of Altos Sur of Jalisco. The total number of isolates per spoligotype is in parentheses

Fig. 4

Evolutionary analysis by maximum likelihood using as input the concatenated SNP sequences from WGS of 34 M. bovis isolates from three municipalities of the region Altos Sur of Jalisco, Mexico: (T) Tepatitlán de Morelos, (A) Arandas, and (M) San Miguel el Alto. The tree is rooted to the reference M. bovis AF2122/97 (accession number NC_002945.4)
Evolutionary analysis by maximum likelihood using as input the concatenated SNP sequences from WGS of 34 M. bovis isolates from three municipalities of the region Altos Sur of Jalisco, Mexico: (T) Tepatitlán de Morelos, (A) Arandas, and (M) San Miguel el Alto. The tree is rooted to the reference M. bovis AF2122/97 (accession number NC_002945.4)

Fig. 5

Maximum likelihood phylogenetic reconstruction of the whole-genome SNP sequences of 196 M. bovis isolates from Mexico (Altos Sur isolates are in red)
Maximum likelihood phylogenetic reconstruction of the whole-genome SNP sequences of 196 M. bovis isolates from Mexico (Altos Sur isolates are in red)

Prevalence of BTb by municipality as confirmed by positive isolation of M_ bovis in culture

Municipality Positive cases Negative cases Total animals sampled Regional prevalence Total prevalence
Tepatitlán de Morelos 33 197 230 14.3 9.9
San Miguel el Alto 9 38 47 19.1 2.7
Arandas 8 47 55 14.5 2.4
TOTAL 50 282 332

Spoligotype patterns obtained for isolates of M_ bovis from the region of Altos Sur in Jalisco, Mexico_ The total number of isolates per spoligotype is in parentheses

Type of tissue sampled which showed typical BTb lesions

Type of tissue Number of samples with lesions %
Lymph nodes
      Maxillary 2 2.9
      Retropharyngeal 18 26.4
      Mediastinal 22 32.4
Lung 3 4.4
Liver 1 1.5
Multiple tissue lesions
      retropharyngeal and maxillary 1 1.5
      retropharyngeal and mediastinal 9 13.2
      retropharyngeal and lung 3 4.4
      retropharyngeal, mediastinal and lung 4 5.9
      maxillary and mediastinal 1 1.5
      mediastinal and lung 4 5.9
TOTAL 68 100

Genetic diversity within the three major clades obtained by WGS of M_ bovis isolates from the region of Altos Sur in Jalisco, Mexico

Clade Isolates Spoligotypes Municipalities
I 16 SB0673, SB0971, NF Tepatitlán de Morelos, Arandas, San Miguel el Alto
II 8 SB0130, SB0145, SB1758, SB1216, SB0872 Tepatitlán de Morelos, Arandas, San Miguel el Alto
III 10 SB0121, SB1177 Tepatitlán de Morelos, Arandas, San Miguel el Alto

Contribution of the total number of samples by municipality

Municipality Number of samples Percentage of the total sample size
Tepatitlán de Morelos 230 69
Arandas 55 17
San Miguel el Alto 47 14
TOTAL 332 100
Lingua:
Inglese
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Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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