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Effects of Environmental Stresses on the Growth of Arabidopsis thaliana Rosette Leaves

Xiang Jiao
Xiang Jiao
, 
Chenchen Peng
Chenchen Peng
, 
Huichun Zhang
Huichun Zhang
, 
Zhihui Huang
Zhihui Huang
 oraz 
Yan Zhao
Yan Zhao
   | 16 lip 2022
Journal of Horticultural Research's Cover Image
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Data publikacji: 16 lip 2022
Zakres stron: 87 - 96
Otrzymano: 01 lut 2022
Przyjęty: 01 cze 2022
DOI: https://doi.org/10.2478/johr-2022-0008
Słowa kluczowe
© 2022 Xiang Jiao et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Example images of A. thaliana leaves at 20th day after emergence under various growth environments (temperature; humidity; light intensity). Note: the blue squares in each picture represent calibration plate
Example images of A. thaliana leaves at 20th day after emergence under various growth environments (temperature; humidity; light intensity). Note: the blue squares in each picture represent calibration plate

Figure 2

Scatter plots of leaf 6 areas for each experiment under various growth environments (temperature; humidity; light intensity)
Scatter plots of leaf 6 areas for each experiment under various growth environments (temperature; humidity; light intensity)

Figure 3

The variation trends of the number of leaves with time under various growth environments (temperature; humidity; light intensity)
The variation trends of the number of leaves with time under various growth environments (temperature; humidity; light intensity)

Figure 4

Trend charts of environmental factors level
Trend charts of environmental factors level

Range analysis results of the final size and the number of the rosette leaves of A. thaliana

Trial number Trial factors Estimated results
T H I Final leaf size per mm2 Leaf number
temperature (°C) humidity (%) light intensity (μmol·m−2·s−1)
Trial design 1 22 50 278 129.03 21
2 22 65 92 102.06 16
3 22 80 184 104.26 18
4 25 50 184 174.31 15
5 25 65 278 95.99 15
6 25 80 92 85.90 14
7 28 50 92 84.75 12
8 28 65 184 125.30 11
9 28 80 278 139.18 12
Final leaf size K1 335.35 388.09 272.71
K2 356.2 323.35 403.87
K3 349.23 329.34 364.21
K¯1 {{\bar K}_1} 111.78 129.36 90.90
K¯2 {{\bar K}_2} 118.73 107.78 134.62
K¯3 {{\bar K}_3} 116.41 109.78 121.40
Range R 6.95 21.58 43.72
Leaf number analysis K1 55 48 42
K2 44 42 44
K3 35 44 48
K¯1 {{\bar K}_1} 18 16 14
K¯2 {{\bar K}_2} 15 14 15
K¯3 {{\bar K}_3} 12 15 16
Range R 6 2 2

Analysis of variance for the final rosette leaf size of A. thaliana

Source SS DF MS F-value Fα Sig.
T 75.11 2 37.56 0.026 F0.05(2,2) = 19.0F0.1(2,2) = 9.0
H 853.19 2 426.60 0.30
I 3018.77 2 1509.39 1.06
Error 2860.54 2 1430.27
Total 6807.61 8

Analysis of variance for the number of rosette leaves of A. thaliana

Source SS DF MS F-value Fα Sig.
T 66.89 2 33.45 42.88 F0.05(2,2) = 19.0F0.1(2,2) = 9.0 **
H 6.22 2 3.11 3.99
I 6.22 2 3.11 3.99
Error 1.56 2 0.78
Total 80.89 8

Trial environment parameters of each group

Trial number Trial factors Trial factors
T H I
temperature (°C) humidity (%) light intensity (μmol·m−2·s−1)
1 22 50 278 22 °C; 50%; 278 μmol·m−2·s−1
2 22 65 92 22 °C; 65%; 92 μmol·m−2·s−1
3 22 80 184 22 °C; 80%; 184 μmol·m−2·s−1
4 25 50 184 25 °C; 50%; 184 μmol·m−2·s−1
5 25 65 278 25 °C; 65%; 278 μmol·m−2·s−1
6 25 80 92 25 °C; 80%; 92 μmol·m−2·s−1
7 28 50 92 28 °C; 50%; 92 μmol·m−2·s−1
8 28 65 184 28 °C; 65%; 184 μmol·m−2·s−1
9 28 80 278 28 °C; 80%; 278 μmol·m−2·s−1

Regression equations of leaf number under various growth environments (temperature; humidity; light intensity)

Trial factors Growth regression equation Trial factors Growth regression equation
22 °C; 50%; 278 μmol·m−2·s−1 N = 0.68*t + 0.17 22 °C; 80%; 184 μmol·m−2·s−1 N = 0.65*t + 0.40
22 °C; 65%; 92 μmol·m−2·s−1 N = 0.47*t + 0.22 25 °C; 65%; 278 μmol·m−2·s−1 N = 0.68*t + 0.80
25 °C; 50%; 184 μmol·m−2·s−1 N = 0.67*t + 0.99 25 °C; 80%; 92 μmol·m−2·s−1 N = 0.45*t + 0.61
28 °C; 80%; 278 μmol·m−2·s−1 N = 0.71*t + 0.74 28 °C; 65%; 184 μmol·m−2·s−1 N = 0.57*t + 0.63
28 °C; 50%; 92 μmol·m−2·s−1 N = 0.38*t + 1.53

Optimization table for the environmental factors

Characteristic Combination of optimal growth conditions
Final leaf size 25 °C, 50% humidity, 184 μmol·m−2·s−1
Leaf number 22 °C, 50% humidity, 278 μmol·m−2·s−1
eISSN:
2353-3978
Język:
Angielski
Częstotliwość wydawania:
2 razy w roku
Dziedziny czasopisma:
Life Sciences, Biotechnology, Plant Science, Ecology, other
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