Effects of Environmental Stresses on the Growth of Arabidopsis thaliana Rosette Leaves

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Range analysis results of the final size and the number of the rosette leaves of A_ thaliana

Trial number		Trial factors			Estimated results
		T	H	I	Final leaf size per mm2	Leaf number
		temperature (°C)	humidity (%)	light intensity (μmol·m−2·s−1)
Trial design	1	22	50	278	129.03	21
	2	22	65	92	102.06	16
	3	22	80	184	104.26	18
	4	25	50	184	174.31	15
	5	25	65	278	95.99	15
	6	25	80	92	85.90	14
	7	28	50	92	84.75	12
	8	28	65	184	125.30	11
	9	28	80	278	139.18	12
Final leaf size	K1	335.35	388.09	272.71
	K2	356.2	323.35	403.87
	K3	349.23	329.34	364.21
	K¯1 {{\bar K}_1}	111.78	129.36	90.90
	K¯2 {{\bar K}_2}	118.73	107.78	134.62
	K¯3 {{\bar K}_3}	116.41	109.78	121.40
	Range R	6.95	21.58	43.72
Leaf number analysis	K1	55	48	42
	K2	44	42	44
	K3	35	44	48
	K¯1 {{\bar K}_1}	18	16	14
	K¯2 {{\bar K}_2}	15	14	15
	K¯3 {{\bar K}_3}	12	15	16
	Range R	6	2	2

Analysis of variance for the final rosette leaf size of A_ thaliana

Source	SS	DF	MS	F-value	Fα	Sig.
T	75.11	2	37.56	0.026	F0.05(2,2) = 19.0F0.1(2,2) = 9.0
H	853.19	2	426.60	0.30
I	3018.77	2	1509.39	1.06
Error	2860.54	2	1430.27
Total	6807.61	8

Analysis of variance for the number of rosette leaves of A_ thaliana

Source	SS	DF	MS	F-value	Fα	Sig.
T	66.89	2	33.45	42.88	F0.05(2,2) = 19.0F0.1(2,2) = 9.0	**
H	6.22	2	3.11	3.99
I	6.22	2	3.11	3.99
Error	1.56	2	0.78
Total	80.89	8

Trial environment parameters of each group

Trial number	Trial factors			Trial factors
	T	H	I
	temperature (°C)	humidity (%)	light intensity (μmol·m−2·s−1)
1	22	50	278	22 °C; 50%; 278 μmol·m−2·s−1
2	22	65	92	22 °C; 65%; 92 μmol·m−2·s−1
3	22	80	184	22 °C; 80%; 184 μmol·m−2·s−1
4	25	50	184	25 °C; 50%; 184 μmol·m−2·s−1
5	25	65	278	25 °C; 65%; 278 μmol·m−2·s−1
6	25	80	92	25 °C; 80%; 92 μmol·m−2·s−1
7	28	50	92	28 °C; 50%; 92 μmol·m−2·s−1
8	28	65	184	28 °C; 65%; 184 μmol·m−2·s−1
9	28	80	278	28 °C; 80%; 278 μmol·m−2·s−1

Regression equations of leaf number under various growth environments (temperature; humidity; light intensity)

Trial factors	Growth regression equation	Trial factors	Growth regression equation
22 °C; 50%; 278 μmol·m−2·s−1	N = 0.68*t + 0.17	22 °C; 80%; 184 μmol·m−2·s−1	N = 0.65*t + 0.40
22 °C; 65%; 92 μmol·m−2·s−1	N = 0.47*t + 0.22	25 °C; 65%; 278 μmol·m−2·s−1	N = 0.68*t + 0.80
25 °C; 50%; 184 μmol·m−2·s−1	N = 0.67*t + 0.99	25 °C; 80%; 92 μmol·m−2·s−1	N = 0.45*t + 0.61
28 °C; 80%; 278 μmol·m−2·s−1	N = 0.71*t + 0.74	28 °C; 65%; 184 μmol·m−2·s−1	N = 0.57*t + 0.63
28 °C; 50%; 92 μmol·m−2·s−1	N = 0.38*t + 1.53

Optimization table for the environmental factors

Characteristic	Combination of optimal growth conditions
Final leaf size	25 °C, 50% humidity, 184 μmol·m−2·s−1
Leaf number	22 °C, 50% humidity, 278 μmol·m−2·s−1