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Description of Nothotylenchus savadkoohensis n. sp. (Rhabditida, Anguinidae) from Iran based on morphological and molecular data

Soheila Rezaei
Soheila Rezaei
, 
Ebrahim Pourjam
Ebrahim Pourjam
, 
Mohammad Reza Atighi
Mohammad Reza Atighi
 oraz 
Majid Pedram
Majid Pedram
   | 22 kwi 2024
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Article Category: Research Paper
Data publikacji: 22 kwi 2024
Zakres stron: -
Otrzymano: 22 sty 2024
DOI: https://doi.org/10.2478/jofnem-2024-0015
Słowa kluczowe
© 2024 Soheila Rezaei et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Line drawings of Nothotylenchus savadkoohensis n. sp. A, B: Male and female entire body; C: Anterior body region; D: Female pharyngeal region; E: Male posterior body region; F: Female posterior body region.
Line drawings of Nothotylenchus savadkoohensis n. sp. A, B: Male and female entire body; C: Anterior body region; D: Female pharyngeal region; E: Male posterior body region; F: Female posterior body region.

Figure 2:

Light micrographs of Nothotylenchus savadkoohensis n. sp. A: Anterior body region; B & C: Non-muscular metacorpus; D: Vulva region and postvulval uterine sac; E: Female posterior body region (tail); F: Female tail tip; G: Male posterior body region and bursa; H & I: Short overlapping of pharyngeal bulb; J: Lateral field; K: Spicules (Scale bars: A-I, K=10 μm, J=5 μm).
Light micrographs of Nothotylenchus savadkoohensis n. sp. A: Anterior body region; B & C: Non-muscular metacorpus; D: Vulva region and postvulval uterine sac; E: Female posterior body region (tail); F: Female tail tip; G: Male posterior body region and bursa; H & I: Short overlapping of pharyngeal bulb; J: Lateral field; K: Spicules (Scale bars: A-I, K=10 μm, J=5 μm).

Figure 3:

Bayesian 50% majority rule consensus tree inferred from D2–D3 expansion region of LSU rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the GTR + G + I model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from D2–D3 expansion region of LSU rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the GTR + G + I model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Figure 4:

Bayesian 50% majority rule consensus tree inferred from ITS rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the HKY + G model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.
Bayesian 50% majority rule consensus tree inferred from ITS rDNA sequence of Nothotylenchus savadkoohensis n. sp. from Mazandaran province under the HKY + G model. Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The newly generated sequence of the new species is in bold font.

Morphometrics of Nothotylenchus savadkoohensis n. sp. All measurements are in μm and in the form: mean ± standard deviation (range).

Character Females Males
Holotype Paratypes Paratypes
n - 12 4
L 485 526±80 (379–662) 496.3±8.5 (485–505)
a 37.3 38.4±5.2 (27.1–47.3) 37.9±2.4 (34.6–40.4)
b 4.8 5.4±0.7 (4.2–6.3) 5.2±0.2 (5.0–5.4)
c 9.7 11.1±1.4 (8.4–13.9) 10.1±0.9 (9.2–11.2)
c′ 6.7 6.1±0.6 (4.9–7.3) 6.3±0.9 (5.0–6.9)
V 79.4 81.8±2.3 (76.5–84.0) -
Anterior end to vulva 385 426±74 (290–550) -
Lip region height 3 2.5±0.5 (2.0–3.2) 2.3±0.2 (2.0–2.5)
Lip region width 5 5.0±0.3 (4.8–5.7) 5.0±0.1 (4.8–5.1)
Stylet conus 2 2.1±0.2 (2.0–2.5) 2.2±0.1 (2.0–2.3)
Stylet 6 6.2±0.4 (5.8–6.9) 6.21±0.4 (5.6–6.5)
m 33.3 33.8±2.2 (30.8–37.1) 34.9±1.9 (32.3–36.5)
Anal/cloacal diameter 7.5 8.5±0.5 (8–9) 8.0±0.8 (7.2–9.0)
Maximum body with 13 13.6±0.9 (12–15) 13.1±0.6 (12.5–14.0)
Vulval body width 11 11.9±1.2 (10–14) -
Anal body width 7.5 7.9±3.4 (7.5–9.0 7.7±0.6 (7–8)
Excretory pore 77 76.3±3.4 (68–80) 72.7±1.8 (71.0–74.5)
Hemizonid 71 70.0±1.5 (67–72) 70.7±2.1 (68.0–72.6)
Pharynx 102 97.6±5.6 (90–105) 96.5±5.2 (90–102)
Nerve ring 55 56.6±2.5 (53–62) 60±5 (53–65)
Overlap length 4.9 4.8±0.2 (4.5–5.2) 3.0±0.7 (4.0–4.8)
Postvulval uterine sac (PUS) 15 18.7±3.1 (15–26) -
Vulva to anus 45 45.4±5.7 (40.0–55.5) -
Tail 50 47.8±4.4 (42–55) 49.5±3.3 (45–53)
Spicules - - 13.5±0.4 (13.0–14.5)
Gubernaculum - - 4.8±0.2 (4.5–5.0)
eISSN:
2640-396X
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
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